Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization

Dou, Delong; Zhou, Gang; Liu, H.W.; Zhang, J.; Liu, M.L.; Xiao, Xiongfu; Fei, J.J.; Guan, Xiali; Fan, Yubo

doi:10.1016/j.ijbiomac.2018.11.099

Cited by 60 publications

(29 citation statements)

References 24 publications

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“…It should be noted that PCL/PLGA-PEI scaffolds with sustaining BMP2 release had the best promoting effect on the in vitro differentiation of stem cells. Continuous BMP2 supply, whether free or from polyester loading, can upregulate the expression levels of molecular markers related to the bone differentiation, such as Runx2 [ 54 , 55 ], OPN [ 56 , 57 ], COL-1 [ 58 ] and OCN [ 59 ], as well as simultaneously downregulate the expression of BSP [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

Rahman

Peng

Zhao

et al. 2021

Bioactive Materials

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Hydrophilic bone morphogenetic protein 2 (BMP2) is easily degraded and difficult to load onto hydrophobic carrier materials, which limits the application of polyester materials in bone tissue engineering. Based on soybean-lecithin as an adjuvant biosurfactant, we designed a novel cell-free-scaffold of polymer of poly(ε-caprolactone) and poly(lactide-co-glycolide)-co-polyetherimide with abundant entrapped and continuously released BMP2 for in vivo stem cell-capture and in situ osteogenic induction, avoiding the use of exogenous cells. The optimized bioactive osteo-polyester scaffold (BOPSC), i.e. SBMP-10SC, had a high BMP2 entrapment efficiency of 95.35%. Due to its higher porosity of 83.42%, higher water uptake ratio of 850%, and sustained BMP2 release with polymer degradation, BOPSCs were demonstrated to support excellent in vitro capture, proliferation, migration and osteogenic differentiation of mouse adipose derived mesenchymal stem cells (mADSCs), and performed much better than traditional BMP-10SCs with unmodified BMP2 and single polyester scaffolds (10SCs). Furthermore, in vivo capture and migration of stem cells and differentiation into osteoblasts was observed in mice implanted with BOPSCs without exogenous cells, which enabled allogeneic bone formation with a high bone mineral density and ratios of new bone volume to existing tissue volume after 6 months. The BOPSC is an advanced 3D cell-free platform with sustained BMP2 supply for in situ stem cell capture and osteoinduction in bone tissue engineering with potential for clinical translation.

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Section: Resultsmentioning

confidence: 99%

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

Rahman

Peng

Zhao

et al. 2021

Bioactive Materials

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“…Second, it is necessary to design release sequences. Researchers can modify the encapsulation relationship between the BTE scaffold and cytokine to release the two cytokines in an orderly manner that complies with the natural process and achieves enhanced regeneration effects [ [310] , [311] , [312] , [313] ]. For example, Barati et al developed a dual-factor sequential releasing scaffold, which possessed a hierarchical structure with two delivery systems that could release VEGF in 10 days and BMP-2 in 21 days.…”

Section: Bioactive Molecular Delivery Based On Bone-healing Mechanismmentioning

confidence: 99%

Bone physiological microenvironment and healing mechanism: Basis for future bone-tissue engineering scaffolds

Zhu

Zhang

Miao

et al. 2021

Bioactive Materials

257

214

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Bone-tissue defects affect millions of people worldwide. Despite being common treatment approaches, autologous and allogeneic bone grafting have not achieved the ideal therapeutic effect. This has prompted researchers to explore novel bone-regeneration methods. In recent decades, the development of bone tissue engineering (BTE) scaffolds has been leading the forefront of this field. As researchers have provided deep insights into bone physiology and the bone-healing mechanism, various biomimicking and bioinspired BTE scaffolds have been reported. Now it is necessary to review the progress of natural bone physiology and bone healing mechanism, which will provide more valuable enlightenments for researchers in this field. This work details the physiological microenvironment of the natural bone tissue, bone-healing process, and various biomolecules involved therein. Next, according to the bone physiological microenvironment and the delivery of bioactive factors based on the bone-healing mechanism, it elaborates the biomimetic design of a scaffold, highlighting the designing of BTE scaffolds according to bone biology and providing the rationale for designing next-generation BTE scaffolds that conform to natural bone healing and regeneration.

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“…A direct approach to realize the sequential release is loading different drugs into distinct spatial positions of the carrier. In the absence of external environmental stimuli, the concentration gradient as the only driving force will bring a temporal-staggered release, and the release rates of the two guest molecules are different, although the overlap releasing of drugs is inevitable sometimes [161][162][163][164]. Dou et al obtained an O-carboxymethyl chitosan microspheres (O-CMCS) hydroxyapatite collagen scaffold [164].…”

Section: Sequential Releasementioning

confidence: 99%

Hollow structures as drug carriers: Recognition, response, and release

Zhao

Yang

et al. 2021

Nano Res.

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Hollow structures have demonstrated great potential in drug delivery owing to their privileged structure, such as high surface-to-volume ratio, low density, large cavities, and hierarchical pores. In this review, we provide a comprehensive overview of hollow structured materials applied in targeting recognition, smart response, and drug release, and we have addressed the possible chemical factors and reactions in these three processes. The advantages of hollow nanostructures are summarized as follows: hollow cavity contributes to large loading capacity; a tailored structure helps controllable drug release; variable compounds adapt to flexible application; surface modification facilitates smart responsive release. Especially, because the multiple physical barriers and chemical interactions can be induced by multishells, hollow multishelled structure is considered as a promising material with unique loading and releasing properties. Finally, we conclude this review with some perspectives on the future research and development of the hollow structures as drug carriers.

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Sequential releasing of VEGF and BMP-2 in hydroxyapatite collagen scaffolds for bone tissue engineering: Design and characterization

Cited by 60 publications

References 24 publications

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration

Bone physiological microenvironment and healing mechanism: Basis for future bone-tissue engineering scaffolds

Hollow structures as drug carriers: Recognition, response, and release

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