Sequential production of ligninolytic, xylanolytic, and cellulolytic enzymes by Trametes hirsuta AA-017 under different biomass of Indonesian sorghum accessions-induced cultures

Andriani, Ade; Alika, Maharani,; Yanto, Dede Heri Yuli; Pratiwi, Hartinah; Astuti, Dwi; Nuryana, Isa; Anita, Sita Heris; Juanssilfero, A.B.; Perwitasari, Urip; Pantouw, Carla Frieda; Nurhasanah, Ade Nena; Windiastri, Vincentia Esti; Nugroho, Satya; Widyajayantie, Dwi; Sutiawan, Jajang; Sulistyowati, Yuli; Rahmani, Nanik; Ningrum, Ratih Asmana; Yopi, -

doi:10.1016/j.biteb.2020.100562

Cited by 10 publications

(4 citation statements)

References 57 publications

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“…Brown-rot fungi (BRF) depolymerize preferentially cellulose and hemicellulose, while the lignin fraction is modified only to some extent [ 19 , 22 ]. White-rot fungi (WRF) have the ability to breakdown all wood constituents simultaneously or selectively, with some species showing both degradation patterns [ 19 , 23 ]. WRF are considered the most effective lignocellulosic degraders due to their ability to depolymerize a large amount of lignin to CO 2 and H 2 O, facilitating the access of cellulases and hemicellulases to the carbohydrates of the plant cell wall [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa

Ribeiro Tomé,

Dornelles Parise,

Parise

et al. 2024

Heliyon

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Section: Introductionmentioning

confidence: 99%

Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa

Ribeiro Tomé,

Dornelles Parise,

Parise

et al. 2024

Heliyon

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“…White-rot fungi (WRF) act efficiently in the biodegradation of plant biomass, possessing the metabolic machinery for the breakdown of all plant cell wall polymers (PCW) [6][7][8][9][10][11][12]. The depolymerization of the lignocellulose by WRF is carried out through the production and secretion of hydrolytic and oxidative enzymes belonging to glycoside hydrolases (GH), carbohydrate esterases (CE), pectate lyases (PL), and auxiliary activity oxidoreductases (AA) families, according to the classification in the Carbohydrate Active Enzymes database (CAZymes database) [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Fungi of the genus Trametes (Polyporales, Basidiomycota) are classified as whiterot fungi and have the ability to simultaneously degrade all polymers of the lignocellulose [6][7][8]18,19]. In nature, they act as one of the major decomposers of wood and plant leaf litter, and play a central role in the carbon cycle, soil formation, and, consequently, in the maintenance of forest ecosystems [13,20,21].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Assembly Improves Genome Quality and Completeness of Trametes villosa CCMB561 and Reveals a Huge Potential for Lignocellulose Breakdown

Tomé

Silva

Fonseca

et al. 2022

JoF

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Trametes villosa is a wood-decaying fungus with great potential to be used in the bioconversion of agro-industrial residues and to obtain high-value-added products, such as biofuels. Nonetheless, the lack of high-quality genomic data hampers studies investigating genetic mechanisms and metabolic pathways in T. villosa, hindering its application in industry. Herein, applying a hybrid assembly pipeline using short reads (Illumina HiSeq) and long reads (Oxford Nanopore MinION), we obtained a high-quality genome for the T. villosa CCMB561 and investigated its genetic potential for lignocellulose breakdown. The new genome possesses 143 contigs, N50 of 1,009,271 bp, a total length of 46,748,415 bp, 14,540 protein-coding genes, 22 secondary metabolite gene clusters, and 426 genes encoding Carbohydrate-Active enzymes. Our CAZome annotation and comparative genomic analyses of nine Trametes spp. genomes revealed T. villosa CCMB561 as the species with the highest number of genes encoding lignin-modifying enzymes and a wide array of genes encoding proteins for the breakdown of cellulose, hemicellulose, and pectin. These results bring to light the potential of this isolate to be applied in the bioconversion of lignocellulose and will support future studies on the expression, regulation, and evolution of genes, proteins, and metabolic pathways regarding the bioconversion of lignocellulosic residues.

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“…Lignin mempunyai manfaat antara lain sebagai bahan perekat dan dapat dikembangkan menjadi produk bioenergi bernilai tinggi (Rahayu et al, 2020;Susilowati et al, 2013). Selain itu potensi kandungan lignoselulosa sorgum dapat digunakan sebagai sumber karbon dan penginduksi mikroorganisme untuk menghasilkan berbagai produk seperti enzim-enzim yang terkait dengan biorefinery (Andriani et al, 2020). Pada penelitian sebelumnya telah dilakukan analisis kandungan lignin terhadap 30 aksesi sorgum, dan diperoleh aksesi KS (Konawe Selatan) memiliki lignin tertinggi di antara 30 aksesi ( (Wahyuni et al, 2019).…”

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Seleksi dan Evaluasi Sorgum Mutan Generasi M2 Hasil Radiasi Sinar Gamma untuk Peningkatan Karakter Biomassa

Sulistyowati

Nurhasanah

Rachmat

et al. 2022

JPPT

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Sorgum (Sorghum bicolor L.) merupakan tanaman serealia yang dapat beradaptasi di lahan kering sehingga cukup potensial untuk dikembangkan di Indonesia. Sorgum memiliki banyak manfaat yaitu sebagai bahan pangan, pakan dan industri. Salah satu kandungan dalam sorgum yang dapat dimanfaatkan untuk bahan industri adalah lignin yang dapat digunakan dalam pembuatan particle board dan briket. Pada penelitian sebelumnya telah dilakukan seleksi kandungan lignin terhadap 30 genotipe sorgum dan diperoleh genotipe dengan kandungan lignin tertinggi yaitu KS (Konawe Selatan). Namun genotipe KS memiliki biomassa yang masih rendah, sehingga perlu dilakukan perbaikan untuk meningkatkan biomassa. Mutasi merupakan salah satu cara untuk meningkatkan keragaman genetik. Tujuan penelitian ini adalah untuk mendapatkan galur mutan sorgum yang memiliki karakter yang berhubungan dengan biomassa seperti tinggi tanaman, diameter batang dan bobot batang lebih baik dibanding kontrol. Penelitian dilaksanakan pada bulan Juli – November 2019 di Kebun Percobaan Citayam. Materi genetik yang digunakan adalah galur mutan M2 dari radiasi sinar gamma 300, 400 dan 500 Gy dan tanaman kontrol 0 Gy. Sebanyak 50 malai setiap gray ditanam satu baris per malai dan diamati karakter agronominya. Pengamatan agronomi pada galur mutan M2 menunjukkan bahwa populasi galur mutan 300 Gy memiliki nilai kisaran yang lebih luas dibanding populasi 400 dan 500 Gy serta kontrol. Populasi mutan 300 Gy juga memiliki nilai rataan yang lebih tinggi dibanding populasi mutan 400 dan 500 Gy serta kontrol pada karakter tinggi tanaman 309.18 cm (kontrol 305.33 cm), diameter batang 24.09 mm (kontrol 19.37 mm) , bobot batang 860.73 g (kontrol 507.10 g) , panjang malai 42.55 cm (kontrol 39.95 cm) dan bobot malai 95.52 g (kontrol 64.50 g). Mutasi dengan radiasi sinar gamma dapat meningkatkan keragaman genetik dan seleksi pada galur mutan M2 diperoleh galur-galur yang memiliki tinggi, diameter batang dan bobot batang lebih baik dari kontrol yang menunjukkan adanya peningkatan biomassa.

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Sequential production of ligninolytic, xylanolytic, and cellulolytic enzymes by Trametes hirsuta AA-017 under different biomass of Indonesian sorghum accessions-induced cultures

Cited by 10 publications

References 57 publications

Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa

Pure lignin induces overexpression of cytochrome P450 (CYP) encoding genes and brings insights into the lignocellulose depolymerization by Trametes villosa

Hybrid Assembly Improves Genome Quality and Completeness of Trametes villosa CCMB561 and Reveals a Huge Potential for Lignocellulose Breakdown

Seleksi dan Evaluasi Sorgum Mutan Generasi M2 Hasil Radiasi Sinar Gamma untuk Peningkatan Karakter Biomassa

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