Sequential Optimization Approach for Enhanced Production of Antimicrobial Compound from <I>Streptomyces rochei</I> BKM-4

Abstract

The main objective of the present work is to demonstrate the efficiency of multi-factorial experimental designs to elucidate factors affecting the antimicrobial compound production by Streptomyces rochei BKM-4 isolated from an Eastern Ghats soil. Combinations of Plackett -Burman design (PBD) and Box -Behnken design (BBD) have been carried out for the optimization of various factors for antimicrobial production. Among 9 factors, yeast extract, D-glucose and incubation periods were selected due to significant positive effect on antimicrobial compound production. Box -Behnken design, a response surface methodology, was used for further optimization of these selected factors for better output. Data were analyzed, a second order polynomial model was established to identify the relationship between the compound yield and the selected factors. The media formulations were optimized having the factors such as yeast extract 1.10%, D-glucose 0.98% and incubation periods 9.05 days. The maximum experimental response for antimicrobial compound production was 262.42 µg/mL whereas the predicted value was 261.35 µg/ mL. The high correlation among the predicted and observed values indicated the validity of the statistical design. The combined statistical method enabled rapid identification and integration of key medium parameters for optimizing secondary metabolite production and might be very useful in pharmaceutical screening programs.

Insecticidal activity of a novel fatty acid amide derivative from Streptomyces species against Helicoverpa armigera

“…Ōmura (2011) reported a wide range of secondary metabolites identified from bacteria, fungi and actinomycetes. Reports on bioactive metabolites of Streptomyces from forest soil (Balachandran et al 2015;Velayudam & Murugan 2015), marine (Sánchez López et al 2003;Rashad et al 2015) and terrestrial environments (Raju et al 2013;Duraipandiyan et al 2014) Since, the identified compound is a novel fatty acid amide derivative, comparison of its biological activity could be more reasonable with related fatty acids and its amide derivatives. Fatty acid amides and their derivatives exhibited a broad-spectrum antimicrobial activity against human pathogenic bacteria, yeasts and moulds (Novak et al 1969;Kabara et al 1972;omura et al 1974).…”

Growth optimization of mangrove Streptomyces for enhancement of actinorhodin production

“…Velayudham ans Murugan [20] mentioned that different glucose concentration will affect the production of antibiotics. El-sayed et al [21] also added that altering glucose concentration in fed batch culture can yield maximum production of natamycin, produced from Streptomyces natalensis.…”