Sequential-injection spectrophotometric determination of amino acids using 2,4,6-trinitrobenzenesulphonic acid

Ultrasonic-assisted derivatization reaction of amino acids prior to their determination in urine by using single-drop microextraction in conjunction with gas chromatography

“…Flow injection and sequential injection-based analyzers have been proved capable of monitoring amino acids but these approaches were not intended for complete profiling. Only total amino acid analysis [2,3] or the assay of a limited number of individual amino acids is possible [4][5][6].…”

Sequential injection analysis

“…Elevated temperature (50 °C) and stopped flow (180 s) were employed by van Staden and McCormack 88 to overcome slow reaction kinetics in the determination of total amino acid content in soya-beans; however, only the analysis of aqueous standards was reported. Fang et al 89,90 employed SIA for the introduction of sample and buffer into a microfluidic capillary electrophoresis system and demonstrated the separation of arginine, phenylalanine and glycine labelled with fluorescein isothiocyanate.…”