Sequential Injection Analysis of Anionic Surfactants Using On-line Preconcentration Technique and a Microfluidic Polymer Chip with an Embedded Ion-Selective Electrode as a Detector

Abstract

A sequential injection analysis (SIA) system with on-line preconcentration technique using a microfluidic chip with an embedded anionic surfactant ion-selective electrode (AS-ISE) as a detector, was developed for the determination of anionic surfactants. Under the SIA system with no on-line preconcentration technique, the AS-ISE in the SIA system showed a linear relationship between peak heights and logarithmic concentrations of AS ion such as dodecylbenzene sulfonate (DBS) ion with a Nernstian slope of 60.4 mV decade −1 in a concentration range from 3.0 × 10 −6 to 1.0 × 10 −3 mol dm. For the determination of trace levels of ASs, on-line preconcentration technique with solid-phase extraction process was incorporated into the SIA system. By using the on-line preconcentration technique in the SIA system, DBS ion in a concentration range from 1.0 × 10 −7 to 3.0 × 10 −6 mol dm −3 was successfully determined. The recovery for DBS ion added to river water samples was ca. 91-97% using on-line preconcentration technique in the SIA system. The result shows that the present SIA system with the on-line preconcentration technique can be applicable to the determination of the level of AS ion in river and tap water samples.

Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector

“…On the other hand, sequential injection analysis (SIA) method is an analytical method in which a sample solution and a reagent solution are injected sequentially into a holding coil (HC) using a syringe pump (SP) and a multi-port selection valve (SV) controlled by a computer (PC) to induce mixing and chemical reactions through bidirectional flow, and the reaction products are led to a detector for measurement. [11][12][13][14][15][16][17][18][19][20] Unlike the flow injection analysis (FIA) method, the SIA method does not require continuous pumping of reagent solutions, and thus has the advantage of extremely low reagent consumption, making it an analysis method that conforms to the zero-emission concept.…”

Determination of Lactate by Sequential Injection Analysis Using a Fluoride Ion-selective Electrode Detector

“…[2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] Among the electrochemical sensors, potentiometry using ion-selective electrodes (ISEs) has very simple analytical procedures and low-cost apparatus and high selectivity to a primary ion, and applicable method to turbid and colored samples. [17][18][19][20] Siddiqi reported a potentiometric determination method of glucose using F ¹ ISE. In the method, F ¹ ion generated by the reaction of glucose, glucose oxidase and horseradish peroxidase (HRP) in the presence of the fluoroaromatic compounds is detected by the F ¹ ISE.…”

“…On the other hand, sequential injection analysis (SIA) method has the advantages of automating complicated analysis operations and reducing the amounts of reagents and waste, compared with conventional FIA methods. [20][21][22][23][24] In the SIA method, a sample and a reagent of strictly determined small volume (microliter order) are sequentially injected into a holding coil using a personal computercontrolled syringe pump and a multi-port valve, and mixing and chemical reaction are performed by bidirectional flow and the reaction product are detected. Contrastingly, FIA is an instrumental analysis method in which a sample solution is introduced into the flow of a reagent solution to induce a chemical reaction and the reaction product is detected.…”

Development of an Optode Detector for Determination of Anionic Surfactants by Flow Injection Analysis

“…In addition, many toxic organic solvents such as chloroform are often used in the methods. Considering the above, there is a need for a simpler method based on chemical sensors such as ion selective electrodes (ISEs), [9][10][11][12][13][14][15][16] optical sensors (optodes) [17][18][19][20][21][22][23][24][25][26][27][28] and fiber optic sensors 29,30 to measure ASs without the use of toxic organic solvents.…”