Sequential imaging and analysis of erythrocyte aggregation

Jayavanth, Sanjay; Singh, Megha; Jagadeesan, K

doi:10.1109/mmb.2002.1002372

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…4E, the blood volume delivered of the malaria-infected blood sample was smaller than that of the normal blood sample. Based on a previous study, 66 the low deformability of malaria-infected RBCs is influenced by their morphological changes.…”

Section: Deformability Measurement Of Malaria-infected Blood Samplesmentioning

confidence: 99%

Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations

Kang

Lee

2016

Analyst

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Red blood cell (RBC) deformability has been considered a potential biomarker for monitoring pathological disorders. High throughput and detection of subpopulations in RBCs are essential in the measurement of RBC deformability. In this paper, we propose a new method to measure RBC deformability by evaluating temporal variations in the average velocity of blood flow and image intensity of successively clogged RBCs in the microfluidic channel array for specific time durations. In addition, to effectively detect differences in subpopulations of RBCs, an air compliance effect is employed by adding an air cavity into a disposable syringe. The syringe was equally filled with a blood sample (V(blood) = 0.3 mL, hematocrit = 50%) and air (V(air) = 0.3 mL). Owing to the air compliance effect, blood flow in the microfluidic device behaved transiently depending on the fluidic resistance in the microfluidic device. Based on the transient behaviors of blood flows, the deformability of RBCs is quantified by evaluating three representative parameters, namely, minimum value of the average velocity of blood flow, clogging index, and delivered blood volume. The proposed method was applied to measure the deformability of blood samples consisting of homogeneous RBCs fixed with four different concentrations of glutaraldehyde solution (0%-0.23%). The proposed method was also employed to evaluate the deformability of blood samples partially mixed with normal RBCs and hardened RBCs. Thereafter, the deformability of RBCs infected by human malaria parasite Plasmodium falciparum was measured. As a result, the three parameters significantly varied, depending on the degree of deformability. In addition, the deformability measurement of blood samples was successfully completed in a short time (∼10 min). Therefore, the proposed method has significant potential in deformability measurement of blood samples containing hematological diseases with high throughput and precise detection of subpopulations in RBCs.

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Section: Deformability Measurement Of Malaria-infected Blood Samplesmentioning

confidence: 99%

Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations

Kang

Lee

2016

Analyst

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“…The objective of the present work is to determine the aggregation parameters of erythrocytes in blood samples of diabetic patients, with normal-cholesterol and hyper-cholesterol concentration, and to compare with healthy subjects in order to study the influence of cholesterol in diabetes mellitus on aggregation of erythrocytes. The technique has been used to measure the aggregation in various conditions like malaria [21], jaundice [22] and blood storage [23].…”

Section: Introductionmentioning

confidence: 99%

Alterations in Aggregation Parameters of Erythrocytes Due to Hyper Cholesterol in Type-2 Diabetes Mellitus

Babu¹

2009

TOCVJ

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The aggregation parameters of erythrocytes in hyperglycemic subjects with normal cholesterol and with hyper cholesterol were analyzed, and compared with control, by optical scattering under dynamic conditions. Aggregation of erythrocytes is determined by online erythrocyte aggregometer, based on sequential analysis of transmitted laser light intensity after passing through the erythrocyte suspension. The aggregation mechanism in terms of various parameters like process initiation time (PIT), process completion time (PCT), aggregation size index (ASI), effective number of cells (ENC), and effective cellular sedimentation duration (ECSD) is determined. The aggregation parameters thus obtained are used to describe quantitatively the dynamic nature of aggregation process under gravitational sedimentation. The present data show that, in diabetes mellitus with normal cholesterol, the aggregation of erythrocytes is increased and in diabetes with hyper cholesterol, the aggregation is further increased significantly. These changes may significantly affect the flow properties of erythrocytes in microcirculation in diabetes mellitus with hyper cholesterol.

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Sequential imaging and analysis of erythrocyte aggregation

Cited by 2 publications

References 9 publications

Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations

Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations

Alterations in Aggregation Parameters of Erythrocytes Due to Hyper Cholesterol in Type-2 Diabetes Mellitus

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