Sequential extractions: A new way for protein quantification—data from peanut allergens

Zhou, Ningling; Li, Wenying; Wu, Zhihua; Li, Xin; Yang, Anshu; Tong, Ping

doi:10.1016/j.ab.2015.05.013

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…Samples were extracted as modified from Zhou et al. [ 14 ] In brief, 50 mg of each defatted sample was extracted in 1 mL of 2D buffer (50 m m Tris‐HCl, pH 8.8 containing 50 m m DTT, 5 m urea, 2 m thiourea, 10 m m EDTA, 2% w/v 3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate, and 4% w/v PVP cross‐linked). Suspensions were vortexed, incubated in a heated sonicating water bath (60 °C, 20 min), and the extracts were clarified by centrifugation (14 000 x g , 20 min).…”

Section: Methodsmentioning

confidence: 99%

Shellfish Tropomyosin IgE Cross‐Reactivity Differs Among Edible Insect Species

Palmer

Marsh

Lü

et al. 2020

Molecular Nutrition Food Res

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Scope: Insects are a potentially environmentally friendly alternative dietary protein source to supplement mammalian and fish sources, but potential allergenic risks are a concern. Consumption of insects may result in anaphylaxis and has been implicated in cross-reactivity with shellfish. Many allergenic proteins may be involved in cross-reactivity, including tropomyosin (TM). The uniformity of TM cross-reactivity among edible insects is unknown. Candidate edible insects for variability in shellfish IgE cross-reactivity are investigated. Methods and results: Selected insects and known related sources of allergens are extracted and probed by immunoblot with sera/plasma from patients sensitized to insects or shellfish. Quantification of TM in these extracts is performed using mass spectrometry. A comparison of the quantity of TM and the IgE reactivity of TM from these insects is performed. Distinct patterns of IgE cross-reactivity are observed with three insect species showing diminished reactivity. This pattern is not consistent with the amount of TM present in these insects, or with overall sequence homology. Conclusion: Insects display a diversity of TM-associated IgE reactivity. It is likely that minor sequence features and/or structural effects are primarily responsible. Additionally, it is demonstrated that some insect species may present significantly less IgE cross-reactivity to shrimp than do others.

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Section: Methodsmentioning

confidence: 99%