Sequential Expression of Bone Matrix Proteins During Rat Calvaria Osteoblast Differentiation and Bone Nodule Formation In Vitro

Nefussi, Jean-Raphaël; Brami, G; Modrowski, Dominique; Oboeuf, M.; Forest, Nadine

doi:10.1177/002215549704500402

Cited by 89 publications

(90 citation statements)

References 61 publications

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“…ALP gene expression was significantly depressed, whereas OC was increased from stage 2 to 3 (Owen et al, 1990). This formulation is supported by several osteogenesis studies (Harris et al, 1994;Nefussi et al, 1997;Yamamoto et al, 2002;Yamamoto et al, 2003;Siggelkow et al, 2004). The ALP and OC mRNA expression patterns in DFAT cells in the present study were very similar to their reports.…”

Section: Discussionsupporting

confidence: 91%

Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Trans-Differentiate into Osteoblasts In Vitro and In Vivo only by All-Trans Retinoic Acid

Oki

Watanabe

Endo

et al. 2008

Cell Struct. Funct.

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ABSTRACT. We investigated whether de-differentiated fat (DFAT) cells, a mature adipocyte-derived preadipocyte cell line, can be induced to trans-differentiate into osteoblasts in vitro and in vivo. All-trans retinoic acid (RA) induced expression of osteoblast-specific mRNAs encoding Cbfa1/Runx2, osterix, alkaline phosphatase, osteopontin, parathyroid hormone receptor, and osteocalcin in the DFAT cells, but did not induce the expression of adipocytespecific mRNAs encoding PPARγ2, C/EBPα, and GLUT4. Moreover, alkaline phosphatase activity was expressed in DFAT cells and the cells underwent mineralization of the bone matrix in vitro. Furthermore, when DFAT cells were transplanted subcutaneously into C57BL/6N mice in diffusion chambers, these cells formed ectopic osteoid tissue without any host cell-invasion of the chambers. These results indicate that DFAT cells derived from mature adipocytes can be converted into fully differentiated osteoblasts in vitro and in vivo using RA. DFAT cells provide a unique model for studying the lineage commitment of the adipocytes and osteoblasts derived from mesenchymal stem cells. Identification of the pathways that regulate these processes could lead to the development of new therapeutic strategies for control of unwarranted growth of bone and adipose tissue.

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Section: Discussionsupporting

confidence: 91%

Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Trans-Differentiate into Osteoblasts In Vitro and In Vivo only by All-Trans Retinoic Acid

Oki

Watanabe

Endo

et al. 2008

Cell Struct. Funct.

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“…In vitro osteogenic differentiation of primary osteoblasts [8][9][10][11][12][13]44] and ESCs [17][18][19][20][21] have both been well described, but there have been few comparative studies. In this study, both cell types showed expression of markers indicative of osteogenic differentiation and formed nodules comprising Values corrected to proportion of osteogenic control.…”

Section: Discussionmentioning

confidence: 99%

“…The ECM deposited by osteoblasts in vitro has been shown to include collagen-I (col-I), fibronectin, osteocalcin (OCN), and osteopontin (OPN), and staining for these proteins is often most predominant around the mineralized nodules [10][11][12][13]. The process of osteogenesis is coordinated by various transcription factors, with Runx2 and osterix being regarded as key regulators [14][15][16].…”

mentioning

confidence: 99%

Comparison of Osteogenic Differentiation of Embryonic Stem Cells and Primary Osteoblasts Revealed by Responses to IL-1β, TNF-α, and IFN-γ

Sidney

Kirkham

Buttery

2014

Stem Cells and Development

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There are well-established approaches for osteogenic differentiation of embryonic stem cells (ESCs), but few show direct comparison with primary osteoblasts or demonstrate differences in response to external factors. Here, we show comparative analysis of in vitro osteogenic differentiation of mouse ESC (osteo-mESC) and mouse primary osteoblasts. Both cell types formed mineralized bone nodules and produced osteogenic extracellular matrix, based on immunostaining for osteopontin and osteocalcin. However, there were marked differences in the morphology of osteo-mESCs and levels of mRNA expression for osteogenic genes. In response to the addition of proinflammatory cytokines interleukin-1b, tumor necrosis factor-a, and interferon-g to the culture medium, primary osteoblasts showed increased production of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) at early time points and decreases in cell viability. In contrast, osteo-mESCs maintained viability and did not produce NO and PGE 2 until day 21. The formation of bone nodules by primary osteoblasts was reduced markedly after cytokine stimulation but was unaffected in osteo-mESCs. Cell sorting of osteo-mESCs by cadherin-11 (cad-11) showed clear osteogenesis of cad-11+ cells compared to unsorted osteo-mESCs and cad-11 -cells. Moreover, the cad-11 + cells showed a significant response to cytokines, similar to primary osteoblasts. Overall, these results show that while osteo-mESC cultures, without specific cell sorting, show characteristics of osteoblasts, there are also marked differences, notably in their responses to cytokine stimuli. These findings are relevant to understanding the differentiation of stem cells and especially developing in vitro models of disease, testing new drugs, and developing cell therapies.

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“…For the osteoblasts, osteoid matrix, mineralized matrix and chondroblasts there was an increase in the protein with strongly positive labeling. The increase in BSP expression, after disjunction, may be justified by the fact that this protein participates in the process of cell differentiation and activities, as well as maturation and mineralization of the bone matrix [13,38].…”

Section: International Journal Of Dental Sciences and Researchmentioning

confidence: 99%

“…The main noncollagenous bone matrix proteins are osteonectin (ONC), osteopontin (OPN), bone sialoprotein (BSP) and osteocalcin (OCC). The functions of these proteins are related to the regulation of mineralization of collagen fibers and modulation of the division, migration, differentiation and maturation of bone cells and are synthesized in different stages of osteogenesis [13].…”

Section: Introductionmentioning

confidence: 99%

Study of the Tissue Reaction of the Region of the Midpalatine Suture of Rats after Disjunction with Spring Anchored in Mini-Implants

Osório¹,

Reis²,

Osorio³

et al. 2017

IJDSR

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Midpalatine suture disjunction is a routine procedure in the orthodontic clinic; however, the cellular events involved in the procedure have not yet been completely explained. The aim of this study was to verify the tissue events, particularly those related to osteogenic differentiation, which occur in the region of the palatal suture after disjunction, by means of histologic and immunohistochemical analyses of the following noncollagenous proteins: BSP, OCC, OPN and ONC. In this study, 10 male Wistar rats aged two months and weighing approximately 250 g were used. The animals were divided into two groups of five animals: Group I -control (mini-implant placement) and group II -experimental (placement of mini-implant and disjunction spring made of 0.60mm steel wire, with an initial load of 2N (200 grams)). After 21 days, the animals were sacrificed and histological and immunohistochemical analyses were performed for the noncollagenous proteins. The use of the spring anchored to mini-implants promoted the disjunction of the median palatal suture, with a V-shaped opening, in which the base faced the superior region (at the nasal cavity) and the vertex was in the inferior region of the oral cavity (at the palatal bone). It could be concluded that after disjunction, there was presence of osteoblasts in an osteoid matrix in the region of the median palatal suture, and the immunohistochemical expression of the noncollagenous proteins increased in all the analyzed parameters, expression being most significant for ONC.

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Sequential Expression of Bone Matrix Proteins During Rat Calvaria Osteoblast Differentiation and Bone Nodule Formation In Vitro

Cited by 89 publications

References 61 publications

Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Trans-Differentiate into Osteoblasts <i>In Vitro</i> and <i>In Vivo</i> only by All-Trans Retinoic Acid

Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Trans-Differentiate into Osteoblasts <i>In Vitro</i> and <i>In Vivo</i> only by All-Trans Retinoic Acid

Comparison of Osteogenic Differentiation of Embryonic Stem Cells and Primary Osteoblasts Revealed by Responses to IL-1β, TNF-α, and IFN-γ

Study of the Tissue Reaction of the Region of the Midpalatine Suture of Rats after Disjunction with Spring Anchored in Mini-Implants

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