Sequential expression and redundancy of Pitx2 and Pitx3 genes during muscle development

L’honoré, Aurore; Coulon, Vincent; Marcil, Alexandre; Lebel, Mélanie; Lafrance‐Vanasse, Julien; Gage, Philip J.; Camper, Sally A.; Drouin, Jacques

doi:10.1016/j.ydbio.2007.04.034

Cited by 77 publications

(71 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6D). Collectively, these data are in agreement with the expression of Pitx3 in differentiating muscle cells (25) and with a scheme in which Pitx3 expression is subject to positive feedback up-regulation by MyoD during muscle cell differentiation.…”

Section: Myogenic Bhlh Regulatory Factors Activate the Pitx3 Musclesupporting

confidence: 86%

“…It thus appears that Pitx3 is very widely expressed in most developing skeletal muscles. We provided a detailed investigation of this expression profile elsewhere (25).…”

Section: Pitx3 Is Expressed In Developing Muscles Of Aphakia Mice-inmentioning

confidence: 99%

“…A Muscle-specific Pitx3 Exon 1-Since the ak deletion removes the brain and eye (lens) exon 1 and the upstream region of the previously described Pitx3 gene (31), we reasoned (25).…”

Section: Pitx3 Is Expressed In Developing Muscles Of Aphakia Mice-inmentioning

confidence: 99%

“…In addition, the maintenance of Pitx2 expression in Pitx3 Ϫ/Ϫ muscles suggests that at least one Pitx factor must be present for maintenance of the myogenic programs (25) and that Pitx2 may contribute in part to activation of Pitx3 expression. The earliest Pitx gene expressed during muscle cell differentiation is Pitx2 with expression in proliferating muscle pro- genitors (24,25,39). This early expression is progressively replaced by Pitx3 in differentiated and postmitotic muscle cells (25).…”

Section: Role Of Pitx Transcription Factors In the Program Of Musclementioning

confidence: 99%

“…5 Muscle expression of these genes was not studied systematically until recently, as we conducted a detailed analysis of their expression patterns in early precursors and in developing muscle (25). It thus appears that Pitx3 is expressed in all skeletal muscles.…”

mentioning

confidence: 99%

See 4 more Smart Citations

A Muscle-specific Promoter Directs Pitx3 Gene Expression in Skeletal Muscle Cells

Coulon

L’honoré

Ouimette

et al. 2007

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

The Pitx homeobox transcription factor genes have been implicated in different developmental processes, including determination of hind limb identity for Pitx1, left-right asymmetry for Pitx2, and eye development and survival of midbrain dopaminergic neurons for Pitx3. Pitx1 and Pitx2 have partly redundant activities in craniofacial development, including in pituitary organogenesis, as indicated by their names. These genes also exhibit redundant activities in the control of hind limb bud growth. Recent studies have shown expression of the three Pitx genes in muscle, with Pitx3 being the most widely expressed in all skeletal muscles. We now report the identification of a muscle-specific promoter within the Pitx3 gene that is situated between the first exon for eye and brain expression and exon 2 that contains the initiator ATG codon. Sequences proximal to this muscle-specific exon 1 are essential and sufficient to confer muscle-specific expression in transgenic mice, they are responsive to myogenic basic helix-loop-helix regulatory factors, and they recruit these factors in vivo. In agreement with exclusive use of the muscle-specific promoter in aphakia mice that are deleted of the brain promoter, the trimethyl-lysine 4 histone H3 promoter signature shifts to this promoter in embryonic day 13 ak limb bud muscle cells. Myogenic basic helix-loophelix regulatory factor activation of Pitx3 transcription may be part of a positive feedback loop contributing to establishment of the myogenic program.

show abstract

Section: Myogenic Bhlh Regulatory Factors Activate the Pitx3 Musclesupporting

confidence: 86%

“…It thus appears that Pitx3 is very widely expressed in most developing skeletal muscles. We provided a detailed investigation of this expression profile elsewhere (25).…”

Section: Pitx3 Is Expressed In Developing Muscles Of Aphakia Mice-inmentioning

confidence: 99%

“…A Muscle-specific Pitx3 Exon 1-Since the ak deletion removes the brain and eye (lens) exon 1 and the upstream region of the previously described Pitx3 gene (31), we reasoned (25).…”

Section: Pitx3 Is Expressed In Developing Muscles Of Aphakia Mice-inmentioning

confidence: 99%

Section: Role Of Pitx Transcription Factors In the Program Of Musclementioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

A Muscle-specific Promoter Directs Pitx3 Gene Expression in Skeletal Muscle Cells

Coulon

L’honoré

Ouimette

et al. 2007

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Identification of novel transcription‐regulating genes expressed during murine molar development

Uchibe

Shimizu

Yokoyama

et al. 2012

Developmental Dynamics

View full text Add to dashboard Cite

Background: The mechanism of tooth development is a complex process regulated by numerous genes including transcription factors, growth factors, and other intra-and extracellular molecules. Especially, transcription factors play a central role in gene expression, regulating a wide spectrum of biological processes including organogenesis. Substantial evidence has been demonstrated by a number of studies using genetically engineered animal models. However, detailed molecular mechanisms of tooth development have not been completely elucidated, partially because numerous genes that play essential roles in tooth development remain unidentified. Results: In this study, we conducted an expression-based screening using gene expression database and in situ hybridization assays. Based on the gene expression database ''EMBRYS,'' 207 out of 1,520 genes were expressed in the maxillary and/or mandibular processes and thus were selected for further analysis by section in situ hybridization. Among these candidates, 28 genes were newly identified as potential factors associated with tooth development by in situ hybridization assays with frontal sections of embryonic day 13.5 and 14.5 mouse embryos. The expression patterns were also examined at embryonic day 16.5 and 18.5. Conclusions: These results will contribute to elucidating the mechanisms of tooth development and to improving the technology for regeneration of tooth. Developmental Dynamics 241:1217-1226, 2012. V C 2012 Wiley Periodicals, Inc.Key words: tooth development; in situ hybridization; epithelium; mesenchyme; transcription factor; expression pattern Key findings:1520 transcription-regulating genes were screened to identify new genes in tooth development. 28 genes were expressed in the developing molar. The expression patterns in the tooth germ were examined through the embryonic tooth development.

show abstract

Xenopus pitx3 target genes lhx1 and xnr5 are identified using a novel three‐fluor flow cytometry–based analysis of promoter activation and repression

Hooker¹,

Smoczer

Abbott

et al. 2017

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

Sequential expression and redundancy of Pitx2 and Pitx3 genes during muscle development

Cited by 77 publications

References 56 publications

A Muscle-specific Promoter Directs Pitx3 Gene Expression in Skeletal Muscle Cells

A Muscle-specific Promoter Directs Pitx3 Gene Expression in Skeletal Muscle Cells

Identification of novel transcription‐regulating genes expressed during murine molar development

Xenopus pitx3 target genes lhx1 and xnr5 are identified using a novel three‐fluor flow cytometry–based analysis of promoter activation and repression

Contact Info

Product

Resources

About