2015
DOI: 10.1016/j.joca.2014.11.013
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Sequential exposure to fibroblast growth factors (FGF) 2, 9 and 18 enhances hMSC chondrogenic differentiation

Abstract: Objective To test the effects of sequential exposure to FGF2, 9 and 18 on human Mesenchymal Stem Cells (hMSC) differentiation during in vitro chondrogenesis. Design Control and FGF2-expanded hMSC were cultured in aggregates in the presence of rhFGF9, rhFGF18 or rhFGFR3-specific signaling FGF variants, starting at different times during the chondroinductive program. qRT-PCR and immunocytochemistry were performed at different stages. The aggregate cultures were switched to a hypertrophy-inducing medium along w… Show more

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Cited by 112 publications
(96 citation statements)
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References 41 publications
(68 reference statements)
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“…Meanwhile, we verified that upregulation of miR-195 suppressed FGF-18 expression and the activity level of its downstream FGFR3/ FGFR3 p-Tyr724 , ERK1/2/ERK1/2 p-Thr202/Tyr204 , and SOX9. SOX9, which was well accepted as the upstream regulator of Col2a1 and aggrecan [62,63,64,65,66], was reported as being regulated by FGF-18 according to former studies [67,68]. Furthermore, in the present study, we also showed that ectopic miR-195 could correspondingly regulate the expression of FGF-18 and its downstream SOX9 and further effect the expression of Col2a1 and aggrecan.…”
Section: Discussionsupporting
confidence: 79%
“…Meanwhile, we verified that upregulation of miR-195 suppressed FGF-18 expression and the activity level of its downstream FGFR3/ FGFR3 p-Tyr724 , ERK1/2/ERK1/2 p-Thr202/Tyr204 , and SOX9. SOX9, which was well accepted as the upstream regulator of Col2a1 and aggrecan [62,63,64,65,66], was reported as being regulated by FGF-18 according to former studies [67,68]. Furthermore, in the present study, we also showed that ectopic miR-195 could correspondingly regulate the expression of FGF-18 and its downstream SOX9 and further effect the expression of Col2a1 and aggrecan.…”
Section: Discussionsupporting
confidence: 79%
“…In the present study we utilized MSCs isolated from adult ovine bone marrow and stimulated these with FGF-2 and -18 to examine if we could direct the chondrogenic and osteogenic differentiation of the MSCs in vitro . FGF-2 upregulates Sox9 during cellular expansion of chondroblasts and early activation of chondrogenesis, and augments extracellular matrix (ECM) synthesis [15]. FGF-18 signaling through FGFR3 modulates the expression profiles of established chondrocytes during chondrogenesis, delaying hypertrophy but enhancing anabolic ECM gene expression during early chondrogenesis [16,17].…”
mentioning
confidence: 99%
“…Diffusion dependent depots often demonstrate an initial uncontrolled burst release of drug followed by first order release dictated by the size of drug relative to the pore or mesh size of the carrier. Thus, cells are exposed to drug as soon as the carrier is implanted, which may be undesirable because MSC differentiation is sensitive to the timing of growth factor presentation [45 • ]. …”
Section: Controlled Drug Delivery To Bonementioning
confidence: 99%