Sequential development of meristems in the embryo of Triticum durum. A DNA autoradiographic and cytophotometric analysis

Avanzi, S.; Brunori, A.; D’Amato, Francesco

doi:10.1016/0012-1606(69)90020-7

Cited by 37 publications

(21 citation statements)

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“…In squashes of root meristems of Feulgen stained embryos the mitotic index and the DNA nuclear content were determined. The DNA nuclear content of 100 individual nuclei in each squash was measured with the Barr and Stroud (Glasgow, Scotland) integrating microdensitometer, following the procedure previously described (AVANZI et al 1963 ).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous paper (AvANZI et al 1963) DNA cytophotometry demonstrated the occurrence in the radicle meristem of dry seeds of durum wheat of nuclei with either 2C or 4C DNA contents and nuclei with 2C-4C intermediate values. As labelling experiments, after irradiation of dry seeds, showed that several nuclei enter the first mitotic cycle without DNA synthesis, the 4C nuclei were assumed to be nuclei in the G2 phase in the dry seed state.…”

Section: Introductionmentioning

confidence: 99%

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Relationship Between DNA Synthesis and Water Content During Ripening ofVicia FabaSeed

Brunori¹

1967

Caryologia

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationship Between DNA Synthesis and Water Content During Ripening ofVicia FabaSeed

Brunori¹

1967

Caryologia

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“…In the root apices of some plants mitosis always depletes the Gj population both at the margin of the meristem and at the onset of dormancy. In other plants DNA synthesis continues at the end of the mitotic cycle to produce cells that rest at G2 or undergo endoredupHcation (Avanzi et al, 1963;Avanzi, Brunori and D'Amato, 1969;Brunori, 1967Brunori, , 1971Brunori and D'Amato, 1967;D'Amato, 1972). The problem discussed in this article is that of the control of cycling in cells approaching the end of cycling as they are displaced away from the initials.…”

Section: Introductionmentioning

confidence: 99%

The Cessation of Mitosis at the Margins of a Root Meristem

Clowes

1975

New Phytologist

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SUMMARYThe mean rates of mitosis for all cycling cells and the cell-doubling times have been measured independently by pulse-labelling and by blocking the cell cycle and then used to measure the fraction of cycling cells along the meristem of primary roots of Zea mays. The attenuation of mitotic activity that occurs tow^ards the proximal and distal margins of the meristem is due solely to a reduction in growth fraction and not at all to a change in the rate of cycling. This is contrasted with the situation in the quiescent centre of the meristem where the low rate of cell formation results from both a reduction in growth fraction and prolongation of cell cycles. DNA synthesis outside a normal mitotic cycle begins to occur in regions containing cycling cells on both sides of the quiescent centre.

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“…The surgery performed was similar to that of Avanzi et al (1969). The surgery performed was similar to that of Avanzi et al (1969).…”

mentioning

confidence: 97%

“…Each plant used for autoradiography of thymidine and uridine incorporation was grown for 120 hr and dissected to obtain the apex with young leaves (Avanzi, Brunori, and D'Amato, 1969). The dissected pieces were incubated in 2 ftc/ml methyPH thymidine (S.A. 2.0 c/ftmole, Schwartz Bioresearch) or 2 ftc/ml of 5-3H uridine (S.A. 2.0 c/ftmole, Schwartz Bioresearch) continuously aerated for 1 hr.…”

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confidence: 99%

Early Histogenesis and Semiquantitative Histochemistry of Leaf Initiation in Triticum Aestivum

Evans

Berg

1972

American J of Botany

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The shoot apex of Triticum aestivum cv. Ramona 50 was investigated histologically to describe cell lineages and events during leaf initiation. During histogenesis three periclinal divisions occurred in the first apical layer, with one or two divisions in the second apical layer. This sequence of cell divisions initially occurred in one region and spread laterally in both directions to encircle the meristem. Cells of the third apical layer were not involved in leaf histogenesis. Initially, young leaf primordia were produced from daughter cells of periclinal divisions in the two outer apical layers. Nuclear contents of protein, histone, and RNA in the shoot apex were evaluated as ratios to DNA by means of semiquantitative histochemistry. Daughter cells of periclinal divisions in the outer apical layer which produced the leaf primordia had higher histone/DNA ratios than cells of the remaining meristem. However, protein/DNA and RNA/DNA ratios were similar in both regions. Leaf initial cells had a higher 3H‐thymidine labeling index, a higher RNA synthesis rate, and smaller nuclear volumes than cells of the residual apical meristem.

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Sequential development of meristems in the embryo of Triticum durum. A DNA autoradiographic and cytophotometric analysis

Cited by 37 publications

References 6 publications

Relationship Between DNA Synthesis and Water Content During Ripening ofVicia FabaSeed

Relationship Between DNA Synthesis and Water Content During Ripening ofVicia FabaSeed

The Cessation of Mitosis at the Margins of a Root Meristem

Early Histogenesis and Semiquantitative Histochemistry of Leaf Initiation in Triticum Aestivum

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