Sequential detection of Salmonella typhimurium and Bacillus anthracis spores using magnetoelastic biosensors

Huang, Shichu; Yang, Hong; Lakshmanan, Ramji S.; Johnson, Michael L.; Wan, Jiehui; Chen, I.-H.; Wikle, Howard C.; Petrenko, Valery A.; Barbaree, James M.; Chin, Bryan A.

doi:10.1016/j.bios.2008.09.006

Cited by 115 publications

(81 citation statements)

References 16 publications

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“…[1][2][3][4][5] Similarly, freestanding beams made of ferromagnetic material as a sensor platform have been widely studied for detection of physical, chemical, and biochemical substances. [6][7][8][9][10][11][12][13] These beams operate in the longitudinal mode offering a higher resonant frequencies compared to the transverse mode. Figs.…”

Section: Introductionmentioning

confidence: 99%

Development of FeNiMoB thin film materials for microfabricated magnetoelastic sensors

Cai

Gooneratne

Cha

et al. 2012

Journal of Applied Physics

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MetglasTM 2826MB foils of 25–30 μm thickness with the composition of Fe40Ni38Mo4B18 have been used for magnetoelastic sensors in various applications over many years. This work is directed at the investigation of ∼3 μm thick iron-nickel-molybdenum-boron (FeNiMoB) thin films that are intended for integrated microsystems. The films are deposited on Si substrate by co-sputtering of iron-nickel (FeNi), molybdenum (Mo), and boron (B) targets. The results show that dopants of Mo and B can significantly change the microstructure and magnetic properties of FeNi materials. When FeNi is doped with only Mo its crystal structure changes from polycrystalline to amorphous with the increase of dopant concentration; the transition point is found at about 10 at. % of Mo content. A significant change in anisotropic magnetic properties of FeNi is also observed as the Mo dopant level increases. The coercivity of FeNi films doped with Mo decreases to a value less than one third of the value without dopant. Doping the FeNi with B together with Mo considerably decreases the value of coercivity and the out-of-plane magnetic anisotropy properties, and it also greatly changes the microstructure of the material. In addition, doping B to FeNiMo remarkably reduces the remanence of the material. The film material that is fabricated using an optimized process is magnetically as soft as amorphous MetglasTM 2826MB with a coercivity of less than 40 Am−1. The findings of this study provide us a better understanding of the effects of the compositions and microstructure of FeNiMoB thin film materials on their magnetic properties.

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Section: Introductionmentioning

confidence: 99%

Development of FeNiMoB thin film materials for microfabricated magnetoelastic sensors

Cai

Gooneratne

Cha

et al. 2012

Journal of Applied Physics

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“…To overcome such issues, modified QCM biosensors have been developed based on the immobilization of bacteriophages onto the surface of QCM. For instance, Huang et al 37 expressed a peptide with an affinity for Salmonella typhimurium on the surface of E2 bacteriophages, and immobilize the modified bacteriophage onto a QCM sensor ( Figure 4C). The authors demonstrated a detection limit of 102 cells mL -1 within an assay time of 3 minutes.…”

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confidence: 99%

“…Upon capture of the target pathogen, the ME biosensor is placed under increasing load, and the resulting decrease in resonance frequency is measured as a means to analyze the number of pathogens. 37,42 For example, Li et al 42 immobilized E2 phage, genetically modified to express a peptide against S. typhimurium, onto the surface of ME resonators, and they used the modified ME biosensor to demonstrate the real-time ability to track in situ S. typhimurium levels on the surface of a tomato. The binding of the modified bacteriophage onto S. typhimurium is confirmed via scanning electron microscopy and a detection sensitivity of 50 colony-forming units/mL -1 is obtained.…”

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confidence: 99%

Nanoscale bacteriophage biosensors beyond phage display

Lee¹,

Song²,

Hwang³

et al. 2013

IJN

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“…The level of training or expertise required to perform an analysis using the ME biosensor method is much less than Q-PCR method. Greater sensitivity (lower detection limit) can be obtained by decreasing the size of the ME biosensors [13]. By minimizing the sensor size and directly applying a large number of sensors to the fresh produce, broad coverage of fruits and vegetables can be obtained.…”

Section: Comparison Of Practicability and Applicabilitymentioning

confidence: 99%

“…A phage-based magnetoelastic (ME) biosensor has been developed as a novel, wireless, direct detection method for in-field use [10,13,17,18,27]. ME biosensors have been successfully demonstrated to detect pathogens and spores such as S. typhimurium and Bacillus anthracis.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Phage-Based Magnetoelastic Biosensors with Taqman- Based Quantitative Real-Time PCR for the Detection of Salmonella typhimurium Directly Grown on Tomato Surfaces

Park

Park²,

Wikle³

et al. 2012

J Biosens Bioelectron

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A phage-based magnetoelastic (ME) biosensor method was compared with a TaqMan-based quantitative realtime PCR (Q-PCR) method for the detection of Salmonella typhimurium on tomato surfaces. This ME biosensor method utilizes magnetoelastic resonators coated with E2 filamentous phage to bind with and measure the concentration of S. typhimurium. In this study, standard curves, correlations, and limits of detection (LOD) for the ME biosensor and Q-PCR methods were determined by inoculating tomato surfaces with S. typhimurium suspensions in concentrations ranging from 1 to 8 log CFU/tomato. The LOD for the ME biosensor method and Q-PCR were 3 and 2 log CFU/tomato, respectively. In a direct comparison of the detection methods, S. typhimurium suspensions (3 log CFU/tomato) were inoculated on 65 tomato surfaces, then incubated at 37°C and 100% RH for 24 h. After 24 h, S. typhimurium was positively detected by both methods and the quantified concentrations were nearly the same, (6.35 ± 2.03) and (6.34 ± 0.17) log CFU/tomato respectively for the ME biosensor method and the Q-PCR method, which were significantly greater than the concentration determined by the BGS-plate count method (5.33 ± 0.21). Scanning electron microscopy (SEM) was used to confirm the growth of S. typhimurium on the tomato surfaces and the binding of S. typhimurium on the measurement sensors. This study demonstrated that the ME biosensor method was robust and competitive with Q-PCR for S. typhimurium detection on fresh produce.

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Sequential detection of Salmonella typhimurium and Bacillus anthracis spores using magnetoelastic biosensors

Cited by 115 publications

References 16 publications

Development of FeNiMoB thin film materials for microfabricated magnetoelastic sensors

Development of FeNiMoB thin film materials for microfabricated magnetoelastic sensors

Nanoscale bacteriophage biosensors beyond phage display

Comparison of Phage-Based Magnetoelastic Biosensors with Taqman- Based Quantitative Real-Time PCR for the Detection of Salmonella typhimurium Directly Grown on Tomato Surfaces

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