Sequential delivery of BMP-2 and BMP-7 for bone regeneration using a heparinized collagen membrane

Jo, Jae-Young; Jeong, Sunho; Shin, Young Min; Kang, Seong-Soo; Kim, S.-E.; Jeong, Chang-Mo; Huh, Jung-Bo

doi:10.1016/j.ijom.2015.02.015

Cited by 48 publications

(36 citation statements)

References 29 publications

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“…The clinical significance of a material that is biocompatible, such as the sealants evaluated in this work (MTA and BD), is that when a suitable material is placed in contact with the extracellular space, it is activated within a short time in an accession process generated for transmembrane proteins, which ultimately affects the repair of an injury [27, 28]. …”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials

Escobar‐García

Aguirre-López

Méndez-González

et al. 2016

BioMed Research International

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Objective. The aim of this study was to evaluate the cytotoxicity and cellular adhesion of Mineral Trioxide Aggregate (MTA) and Biodentine (BD) on periodontal ligament fibroblasts (PDL). Methods. PDL cells were obtained from nonerupted third molars and cultured; MTS cellular profusion test was carried out in two groups: MTA and BD, with respective controls at different time periods. Also, the LIVE/DEAD assay was performed at 24 h. For evaluation of cellular adhesion, immunocytochemistry was conducted to discern the expression of Integrin β1 and Vinculin at 12 h and 24 h. Statistical analysis was performed by the Kruskal-Wallis and Mann-Whitney U tests. Results. MTA and BD exhibited living cells up to 7 days. More expressions of Integrin β1 and Vinculin were demonstrated in the control group, followed by BD and MTA, which also showed cellular loss and morphological changes. There was a significant difference in the experimental groups cultured for 5 and 7 days compared with the control, but there was no significant statistical difference between both cements. Conclusions. Neither material was cytotoxic during the time evaluated. There was an increase of cell adhesion through the expression of focal contacts observed in the case of BD, followed by MTA, but not significantly.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials

Escobar‐García

Aguirre-López

Méndez-González

et al. 2016

BioMed Research International

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“…This could explain why the combination of BMP‐2 and BMP‐6 can accelerate the bone formation process (by simultaneously acting on different populations of cells) but does not increase the total amount of bone formed after a longer period. Nevertheless, some groups are already trying to enhance the process by developing systems able to deliver two distinct BMPs in a sequential manner …”

Section: Discussionmentioning

confidence: 99%

Combining bone morphogenetic proteins‐2 and ‐6 has additive effects on osteoblastic differentiation in vitro and accelerates bone formation in vivo

Visser

Bodnarova

Arrabal

et al. 2015

J Biomedical Materials Res

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While only two members of the bone morphogenetic protein subfamily (BMP-2 and -7) are approved to be used in combination with collagen type I in orthopaedic surgery, other BMPs are known to also be highly osteoinductive. Although all the osteogenic BMPs signal through Smad-1/-5/-8 phosphorylation, they show different preferences for the available BMP receptors. In this work we studied the effect of combining two osteogenic BMPs (-2 and -6), which belong to different groups within the subfamily and have different affinities to the existing BMP receptors. Both the growth and in vitro differentiation of MC3T3-E1 mouse preosteoblasts and rat bone marrow-derived mesenchymal stem cells (MSCs) were studied, as well as in vivo ectopic bone formation when the BMPs were intramuscularly implanted in rats with collagen type I sponges as carriers. The results show that these two growth factors have additive effects on the osteoblastic differentiation of cells in vitro and that their combination might be helpful to accelerate in vivo osteogenesis while reducing the amount of each individual BMP used.

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“…[162–164] BMP-2 and BMP-7 (bone morphogenetic protein) are two main osteoinductive factors which have the potential to induce differentiation of osteocytes, followed by mineralization and bone regeneration. [165, 166] Encapsulation of these growth factors on to polymeric scaffolds using various solvents and electric field for fabrication may result in the loss of biological activity. [167] To overcome such challenges, streptavidin was employed for binding biotin-BMP2 and biotin-SAP (self-assembling peptides).…”

Section: Applications Of Nanoscale Avidin Systems In Diagnosis Andmentioning

confidence: 99%

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

Jain

Cheng

2017

Journal of Controlled Release

215

286

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Avidin-biotin interaction is one of the strongest non-covalent interactions in the nature. Avidin and its analogues have therefore been extensively utilized as probes and affinity matrices for a wide variety of applications in biochemical assays, diagnosis, affinity purification, and drug delivery. Recently, there has been a growing interest in exploring this non-covalent interaction in nanoscale drug delivery systems for pharmaceutical agents, including small molecules, proteins, vaccines, monoclonal antibodies, and nucleic acids. Particularly, the ease of fabrication without losing the chemical and biological properties of the coupled moieties makes the avidin-biotin system a versatile platform for nanotechnology. In addition, avidin-based nanoparticles have been investigated as diagnosis systems for various tumors and surface antigens. In this review, we will highlight the various fabrication principles and biomedical applications of avidin-based nanoparticles in drug delivery and diagnosis. The structures and biochemical properties of avidin, biotin and their respective analogues will also be discussed.

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Sequential delivery of BMP-2 and BMP-7 for bone regeneration using a heparinized collagen membrane

Cited by 48 publications

References 29 publications

Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials

Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials

Combining bone morphogenetic proteins‐2 and ‐6 has additive effects on osteoblastic differentiation in vitro and accelerates bone formation in vivo

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

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