Sequential degradation of raw vinasse by a laccase enzyme producing fungus Pleurotus sajor-caju and its ATPS purification

Aqueous two‐phase systems in Latin America: perspective and future trends

“…33,34 Since then, ATPS have become a familiar laboratory-scale approach for a wide range of separation/purification purposes, mainly due to their advantages over conventional extraction techniques (for example, biocompatibility, environmentally-friendly, low cost, volume capacity, ability to handle viscous biomass rich solutions, easy operability and scalability, as well as their usefulness for several biomolecules' classes, especially if the purpose is related to their concentration and purification). [35][36][37] Brazil is probably the country that has most exploited ATPS for isolation, extraction, and/or separation of a large amount of solutes from microbial and plant biomasses, ranging from classical uses in isolating enzymes 38 and proteins, [39][40][41] to the recovery of pigments, 36 monoclonal antibodies, 42 nucleic acids, 43,44 small drugs, 45 and metals. 46 Although some examples of successful use of ATPS on an industrial scale can be found, 35 in Brazil, ATPS have not been used on a commercial scale nor have pilot scale studies been reported.…”

Native fungal strains from Yucatan, an option for treatment of biomethanated vinasse

“…Regarding to raw vinasse, Junior et al (2020) evaluated the color removal on sugarcane vinasse using Pleurotus sajor-caju, these authors reaching values of decolorization between 82 and 92% and the vinasse decolorization was better in the media containing inducers such as ethanol and CuSO4. T. hirsuta (Bm-2) registered a range of decolorization of 68.8 -87.9%, these values are lower than those reported by P. sajor-caju, nevertheless, no inducers for laccase were added to the BV medium and the chemical composition of BV is different to raw vinasse; for example, it is possible that raw vinasse contains small amounts of ethanol that could not be distilled, while BV, as it comes from an anaerobic digestion process, is not present (España-Gamboa et al, 2011).…”

The Effects of Fungal Feed Additives in Animals: A Review

“…In addition to acting as a kind of prebiotic, polysaccharides in edible mushrooms are also well known for their antioxidant and anti-inflammatory capacities [76][77][78][79][80][81]. In mice, the polysaccharides in L. edodes and Phallus atrovolvatus decrease TNF-α, IL-6, IL-1β, blood urea nitrogen, and uric acid levels in blood serum while increasing antioxidant enzymes such as SOD, CAT, and Gpx in the kidney [77,79].…”

“…Peroxidases assist fungi in facing the challenge of toxic phenols and increase their environmental competitiveness [80]. Laccase, one of the most well-known peroxidases in mushrooms, degrades lignin in the culture medium [81]. By degrading lignin, fungi effectively use nutrients that are difficult for other microorganisms to consume [82].…”