Sequential changes in glycolipid expression during human B cell differentiation: enzymatic bases

Taga, Samir; Tétaud, Cécile; Mangeney, Marianne; Tursz, Thomas; Wiels, Joëlle

doi:10.1016/0005-2760(94)00167-w

Cited by 31 publications

(22 citation statements)

References 31 publications

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“…The range of GSL and phospholipids expressed by a specific cell type may have a significant impact on the lipid ligands bound by CD1 molecules. For example, the GSL repertoire in B cells has been shown to undergo serial changes with B cell maturation (32,33). These changes in GSL expression are likely caused by serial changes in the activation of the corresponding glycosyl transfereases as has been previously shown (33).…”

mentioning

confidence: 75%

“…For example, the GSL repertoire in B cells has been shown to undergo serial changes with B cell maturation (32,33). These changes in GSL expression are likely caused by serial changes in the activation of the corresponding glycosyl transfereases as has been previously shown (33). The GSL expression pattern of human lymphocytes, monocytes, and granulocytes has also been shown to be cell specific (34).…”

mentioning

confidence: 83%

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Activation state and intracellular trafficking contribute to the repertoire of endogenous glycosphingolipids presented by CD1d

Muindi

Cernadas

Watts

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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The authors note that, due to a printer's error, on page 5054, right column, second paragraph, eighth line, "Within this clade, we estimated the mean age of the split between the ABC bears and the polar bears to be 152 ky, and the mean age for all polar bears as 134 ky, near the end of the Eemian interglacial period and completely in line with the stratigraphically determined age of the Poolepynten subfossil (11)," should instead appear as "Within this clade, we estimated the mean age of the split between the ABC bears and the polar bears to be 152 ky, and the mean age for all polar bears as 134 ky, near the beginning of the Eemian interglacial period and completely in line with the stratigraphically determined age of the Poolepynten subfossil (11)." This error does not affect the conclusions of the article. This error has been corrected online and in print.www.pnas.org/cgi

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mentioning

confidence: 75%

mentioning

confidence: 83%

Activation state and intracellular trafficking contribute to the repertoire of endogenous glycosphingolipids presented by CD1d

Muindi

Cernadas

Watts

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Lc 3 Cer synthase has been detected in many tissues, including the developing rat brain (14,15,20,46), hematopoietic cells (35,36,(47)(48)(49), and colorectal tissues (50 -52). In all tissues examined, the Lc 3 Cer synthase was identified to be a key enzyme in the extension of the carbohydrate chain on neolactoseries GSLs.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Characterization of UDP-GlcNAc:Lactosylceramide β1,3-N-Acetylglucosaminyltransferase (β3Gn-T5), an Essential Enzyme for the Expression of HNK-1 and Lewis X Epitopes on Glycolipids

Togayachi

Akashima

Ookubo

et al. 2001

Journal of Biological Chemistry

115

108

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A new member of the UDP-N-acetylglucosamine:␤-galactose ␤1,3-N-acetylglucosaminyltransferase (␤3Gn-T) family having the ␤3Gn-T motifs was cloned from rat and human cDNA libraries and named ␤3Gn-T5 based on its position in a phylogenetic tree. We concluded that ␤3Gn-T5 is the most feasible candidate for lactotriaosylceramide (Lc 3 Cer) synthase, an important enzyme which plays a key role in the synthesis of lacto-or neolacto-series carbohydrate chains on glycolipids. ␤3Gn-T5 exhibited strong activity to transfer GlcNAc to glycolipid substrates, such as lactosylceramide (LacCer) and neolactotetraosylceramide (nLc 4 Cer; paragloboside), resulting in the synthesis of Lc 3 Cer and neolactopentaosylceramide (nLc 5 Cer), respectively. A marked decrease in LacCer and increase in nLc 4 Cer was detected in Namalwa cells stably expressing ␤3Gn-T5. This indicated that ␤3Gn-T5 exerted activity to synthesize Lc 3 Cer and decrease LacCer, followed by conversion to nLc 4 Cer via endogenous galactosylation. The following four findings further supported that ␤3Gn-T5 is Lc 3 Cer synthase. 1) The ␤3Gn-T5 transcript levels in various cells were consistent with the activity levels of Lc 3 Cer synthase in those cells. 2) The ␤3Gn-T5 transcript was presented in various tissues and cultured cells. 3) The ␤3Gn-T5 expression was up-regulated by stimulation with retinoic acid and down-regulated with 12-O-tetradecanoylphorbol-13-acetate in HL-60 cells. 4) The changes in ␤3Gn-T5 transcript levels during the rat brain development were determined. Points 2, 3, and 4 were consistent with the Lc 3 Cer synthase activity reported previously.To date, three members of the human ␤1,3-N-acetylglucosaminyltransferase (␤3Gn-T) 1 family (␤3Gn-T2, -T3, and -T4) (1, 2) and five members of the human ␤1,3-galactosyltransferase (␤3Gal-T) family (␤3Gal-T1, -T2, -T3, -T4, and -T5) have been identified (3-6). All of them share amino acid motifs (␤3Gn-T motifs or ␤3Gal-T motifs) in three regions of the catalytic domain. The first, ␤3Gn-T, was cloned by an expression cloning method using an anti-i antibody (7). However, this enzyme is unique in that it does not have the ␤3Gn-T motifs although it transfers GlcNAc to Gal with an ␤1,3-linkage, resulting in the synthesis of polylactosamine chains. It was named iGn-T (7). Thereafter, ␤3Gn-T1 was isolated based on structural similarity with the ␤3Gal-T family (2). We previously reported three additional ␤3Gn-Ts, ␤3Gn-T2, -T3, and -T4, which are also structurally related to the ␤3Gn-T family (1). However, the cDNA sequence of ␤3Gn-T1 was recently corrected by Zhou et al. (see Ref. 2). The corrected sequence of ␤3Gn-T1 was identical to that of ␤3Gn-T2 which was isolated * The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.The nucleotide sequence(s) reported in this paper has been submitted to the DDBJ/GenBank TM /EBI Data Bank with the accession num...

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“…Multiple tissue northern (MTN-H12) blot was obtained from CLONTECH. Eight human cell lines (Ramos, MutuI, BL2, Namalwa, Remb 1 , 8866, T51, and K562) were analyzed because P k synthase activity and antigen expression have been characterized previously (22,23). Total cellular RNA was extracted from cell lines using the RNeasy Midi kit (Qiagen SA).…”

Section: Methodsmentioning

confidence: 99%

“…However, North- Cloning of the P k ␣4-Galactosyltransferaseern analysis of various B cell lines (Fig. 6) shows that ␣4Gal-T1 expression clearly correlates with P k synthase activity and antigen expression in Burkitt's lymphoma cell lines (22,23).…”

Section: Fig 4 Expression Of Full Coding ␣4gal-t1 Variants In Highmentioning

confidence: 99%

Cloning and Expression of the Histo-blood Group PkUDP-galactose:Galβ1–4Glcβ1-Cer α1,4-Galactosyltransferase

Steffensen¹,

Carlier²,

Wiels³

et al. 2000

Journal of Biological Chemistry

115

137

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The molecular genetic basis of the P histo-blood group system has eluded characterization despite extensive studies of the biosynthesis of the P 1 , P, and P k glycolipids. The main controversy has been whether a single or two distinct UDP-Gal:Gal␤1-R 4-␣-galactosyltransferases catalyze the syntheses of the structurally related P 1 and P k antigens. The P 1 polymorphism is linked to 22q11.3-ter. Data base searches with the coding region of an ␣4GlcNAc-transferase identified a novel homologous gene at 22q13.2 designated ␣4Gal-T1. Expression of full coding constructs of ␣4Gal-T1 in insect cells revealed it encoded P k but not P 1 synthase activity. Northern analysis showed expression of the transcript correlating with P k synthase activity and antigen expression in human B cell lines. Transfection of P k -negative Namalwa cells with ␣4Gal-T1 resulted in strong P k expression. A single homozygous missense mutation, M183K, was found in six Swedish individuals of the rare p phenotype, confirming that ␣4Gal-T1 represented the P k gene. Sequence analysis of the coding region of ␣4Gal-T1 in P 1 ؉/؊ individuals did not reveal polymorphisms correlating with P 1 P 2 typing.The P histo-blood group system is the last of the known carbohydrate defined blood group systems for which the molecular genetic basis has not yet been clarified. The P blood group system involves two major blood group phenotypes, P 1 ϩ and P 1 Ϫ, with approximate frequencies of 80% and 20%, respectively (1, 2). P 1 Ϫ individuals normally express the P antigen (P 1 Ϫ is designated P 2 when P antigen expression is demonstrated), but the rare P k phenotype lacks the P antigen, while the rare p phenotype lack both P and P k antigens (for reviews, see Refs. 3-7). The P 1 ϩ phenotype is defined by expression of the neolacto-series glycosphingolipid P 1 (for structures, see Table I) (8). In contrast, the P, P k , and p antigens constitute intermediate steps in biosynthesis of globo-series glycolipids and give rise to P 1 k , P 2 k , and p phenotypes (9). Although the rare P k phenotype shows the same frequency of P 1 antigen expression as individuals expressing the P antigen, the p phenotype is always associated with lack of P 1 antigen expression. Extensive studies of the chemistry, biosynthesis, and genetics of the P blood group system identified the antigens as being exclusively found on glycolipids, with the blood group specificity being synthesized by at least two distinct glycosyltransferase activities; UDP-galactose:␤-D-galactosyl-␤1-R 4-␣-Dgalactosyltransferase (␣4Gal-T) 1 activity(ies) for P k and P 1 syntheses and UDP-GalNAc:Gb 3 3-␤-N-acetylgalactosaminyltransferase activity (EC 2.4.1.79) for P synthesis (for reviews, see Refs. 6 and 7). At least two independent gene loci, P and P 1 P k , are involved in defining these antigens. The P blood group-associated LKE antigen, shown to be the extended sialylated Gal-globoside structure (10), may involve polymorphism in an ␣2,3-sialyltransferase activity.A long-standing controversy has been whether a single...

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Sequential changes in glycolipid expression during human B cell differentiation: enzymatic bases

Cited by 31 publications

References 31 publications

Activation state and intracellular trafficking contribute to the repertoire of endogenous glycosphingolipids presented by CD1d

Activation state and intracellular trafficking contribute to the repertoire of endogenous glycosphingolipids presented by CD1d

Molecular Cloning and Characterization of UDP-GlcNAc:Lactosylceramide β1,3-N-Acetylglucosaminyltransferase (β3Gn-T5), an Essential Enzyme for the Expression of HNK-1 and Lewis X Epitopes on Glycolipids

Cloning and Expression of the Histo-blood Group PkUDP-galactose:Galβ1–4Glcβ1-Cer α1,4-Galactosyltransferase

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