Sequential assessment via daphnia and zebrafish for systematic toxicity screening of heterogeneous substances

Jang, Gun Hyuk; Park, Chang-Beom; Kang, Benedict J; Kim, Young Jun; Lee, Kwan H.

doi:10.1016/j.envpol.2016.06.001

Cited by 35 publications

(18 citation statements)

References 50 publications

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“…In the present study, we examined the ecotoxicological effects of 12 different potential pesticides by using neonates of Daphnia magna and larvae of zebrafish (Danio rerio), which both are excellent aquatic models that have been used extensively in toxicological studies [8]. Previous literature in zebrafish and daphnia had focused on evaluating mortality rate and developmental defects against toxicant exposure in both animal models [2]; however, none of the previous research examined and compared the chemical-induced toxicity at the behavioral level.…”

Section: Introductionmentioning

confidence: 99%

Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations

et al. 2020

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Pesticides are widely used to eradicate insects, weed species, and fungi in agriculture. The half-lives of some pesticides are relatively long and may have the dire potential to induce adverse effects when released into the soil, terrestrial and aquatic systems. To assess the potential adverse effects of pesticide pollution in the aquatic environment, zebrafish (Danio rerio) and Daphnia magna are two excellent animal models because of their transparent bodies, relatively short development processes, and well-established genetic information. Moreover, they are also suitable for performing high-throughput toxicity assays. In this study, we used both zebrafish larvae and water flea daphnia neonates as a model system to explore and compare the potential toxicity by monitoring locomotor activity. Tested animals were exposed to 12 various types of pesticides (three fungicides and 9 insecticides) for 24 h and their corresponding locomotor activities, in terms of distance traveled, burst movement, and rotation were quantified. By adapting principal component analysis (PCA) and hierarchical clustering analysis, we were able to minimize data complexity and compare pesticide toxicity based on locomotor activity for zebrafish and daphnia. Results showed distinct locomotor activity alteration patterns between zebrafish and daphnia towards pesticide exposure. The majority of pesticides tested in this study induced locomotor hypo-activity in daphnia neonates but triggered locomotor hyper-activity in zebrafish larvae. According to our PCA and clustering results, the toxicity for 12 pesticides was grouped into two major groups based on all locomotor activity endpoints collected from both zebrafish and daphnia. In conclusion, all pesticides resulted in swimming alterations in both animal models by either producing hypo-activity, hyperactivity, or other changes in swimming patterns. In addition, zebrafish and daphnia displayed distinct sensitivity and response against different pesticides, and the combinational analysis approach by using a phenomic approach to combine data collected from zebrafish and daphnia provided better resolution for toxicological assessment.

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Section: Introductionmentioning

confidence: 99%

Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations

et al. 2020

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“…1b). To demonstrate liver defects, the sizes and angles of the liver, the slope between the eye and otolith, and the developing liver, were assessed and compared 28 . The groups treated with 50 μM or more of the H 2 O 2 solution exhibited significant differences in size and angle.…”

Section: Resultsmentioning

confidence: 99%

Tailoring H2O2 generation kinetics with magnesium alloys for efficient disinfection on titanium surface

Park

Jang

Jung

et al. 2020

Sci Rep

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A new antibacterial strategy for Ti has been developed without the use of any external antibacterial agents and surface treatments. By combining Mg alloys with Ti, H 2 o 2 , which is an oxidizing agent that kills bacteria, was spontaneously generated near the surface of Ti. Importantly, the H 2 o 2 formation kinetics can be precisely controlled by tailoring the degradation rates of Mg alloys connected to Ti. Through microstructural and electrochemical modification of Mg with alloying elements (Ca, Zn), the degradation rates of Mg alloys were controlled, and the H 2 o 2 release kinetics was accelerated when the degradation rate of Mg alloys increased. With the introduction of an in vivo assessment platform comprised of Escherichia coli (E. coli) and transgenic zebrafish embryos, we are able to design optimized antibacterial systems (Ti-Mg and Ti-Mg-3wt% Zn) that can selectively eradicate E. coli while not harming the survival rate, development, and biological functions of zebrafish embryos. We envision that our antibacterial strategy based on utilization of sacrificial Mg alloys could broaden the current palette of antibacterial platforms for metals. the solution gradually increased over time, reaching 33 μM after 2 h of reaction. In the case of the Ti-Mg-3wt%Ca system, the H 2 O 2 -releasing kinetics were significantly accelerated and a solution of approximately 60 μM H 2 O 2 was formed at the same reaction time. In contrast, adopting the Mg-3wt% Zn alloy in the system reduced the formation rate of H 2 O 2 , and only 23 μM H 2 O 2 was generated after 2 h of reaction. It should be noted that the H 2 O 2 release rate increased in the series of Ti-Mg-3wt% Zn < Ti-Mg < Ti-Mg-3wt%Ca and that this trend was identical to that of the degradation rate of Mg alloys ( Figs. 3f and 4a).Along with the fluorometric H 2 O 2 assay results, cyclic voltammetry (CV) analyses further proved the difference in H 2 O 2 -releasing behavior depending on the type of Mg alloy connected to Ti. The oxygen reduction reaction (ORR) current, which is related to the amount of H 2 O 2 generated near Ti 36,37 , increased in the series of Ti-Mg-3wt% Zn < Ti-Mg < Ti-Mg-3wt%Ca, which led to the same conclusion (Fig. 4b). Specifically, for the Ti-Mg-3wt%Ca system, the ORR current at the applied voltage of −0.2 vs. Ag/AgCl was −1.3 mA/cm 2 , which was approximately 4.3 and 1.5 times higher than that of the Ti-Mg-3wt% Zn and Ti-Mg systems, respectively. The controllable H 2 O 2 -releasing kinetics of the Ti-Mg alloy system through degradation engineering of Mg alloys indicated its high feasibility with regard to selective bacterial remediation. Scientific RepoRtS |(2020) 10:6536 | https://doi.www.nature.com/scientificreports www.nature.com/scientificreports/ zebrafish embryos. ZEISS Stemi 2000, LEICA MZFLIII, ZEISS Imager Z1 and ZEISS axioskop, LEICA S6D, LEICA DMI6000B were used to examine zebrafish embryos at approximately 72 and 96 hpf. The assay was replicated three times. Examination of zebrafish heart functionality.For the control group, the heart...

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“…Toxicity tests for four different glyoxal concentrations (0%, 1.4%, 2.3%, and 6.3%) of Daphnia magna neonates within 8 h after hatching were conducted in six-well cell culture plates (Cellstar ® , greiner bio-one, Frickenhausen, Germany) filled with 10 mL of each test solution [28]. During the experimental period, Daphnia magna neonates were maintained at 23 ± 0.5 °C under a light cycle of 16 h light and 8 h darkness (16L/8D).…”

Section: Methodsmentioning

confidence: 99%

“…Wild-type zebrafish (AB strain) breeding and maintenance was performed under a long photoperiod (16L/8D light/dark cycle) at a 26.5 ± 0.5 °C water temperature [25,28]. Tap water filtered through a Millipore ® 0.22 µm GSWP filter (Merck KGaA, Darmstadt, Germany) after sterilization at 130 °C for 1 h was used as the control group.…”

Section: Methodsmentioning

confidence: 99%

“…Embryos at 8 h post-fertilization were treated with glyoxal-containing cellulose ether for 6 days which is commonly referred to as the embryonic stage. The mortality, hatchability, and developmental abnormalities in each test solution were recorded to assess the embryo toxicity of each glyoxal-containing cellulose ether [25,28]. The embryo toxicity for each cellulose ether was determined in quadruplicate (10 embryos per replicate).…”

Section: Methodsmentioning

confidence: 99%

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An Eco-Safety Assessment of Glyoxal-Containing Cellulose Ether on Freeze-Dried Microbial Strain, Cyanobacteria, Daphnia, and Zebrafish

Park

Song

Choi

et al. 2017

IJERPH

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The objective of this study was to investigate the aquatic-toxic effects of glyoxal-containing cellulose ether with four different glyoxal concentrations (0%, 1.4%, 2.3%, and 6.3%) in response to global chemical regulations, e.g., European Union Classification, Labeling and Packaging (EU CLP). Toxicity tests of glyoxal-containing cellulose ether on 11 different microbial strains, Microcystis aeruginosa, Daphnia magna, and zebrafish embryos were designed as an initial stage of toxicity screening and performed in accordance with standardized toxicity test guidelines. Glyoxal-containing cellulose ether showed no significant toxic effects in the toxicity tests of the 11 freeze-dried microbial strains, Daphnia magna, and zebrafish embryos. Alternatively, 6.3% glyoxal-containing cellulose ether led to a more than 60% reduction in Microcystis aeruginosa growth after 7 days of exposure. Approximately 10% of the developmental abnormalities (e.g., bent spine) in zebrafish embryos were also observed in the group exposed to 6.3% glyoxal-containing cellulose ether after 6 days of exposure. These results show that 6.3% less glyoxal-containing cellulose ether has no acute toxic effects on aquatic organisms. However, 6.3% less glyoxal-containing cellulose ether may affect the health of aquatic organisms with long-term exposure. In order to better evaluate the eco-safety of cellulosic products containing glyoxal, further studies regarding the toxic effects of glyoxal-containing cellulose ether with long-term exposure are required. The results from this study allow us to evaluate the aquatic-toxic effects of glyoxal-containing cellulosic products, under EU chemical regulations, on the health of aquatic organisms.

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Sequential assessment via daphnia and zebrafish for systematic toxicity screening of heterogeneous substances

Cited by 35 publications

References 50 publications

Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations

Multiple Screening of Pesticides Toxicity in Zebrafish and Daphnia Based on Locomotor Activity Alterations

Tailoring H2O2 generation kinetics with magnesium alloys for efficient disinfection on titanium surface

An Eco-Safety Assessment of Glyoxal-Containing Cellulose Ether on Freeze-Dried Microbial Strain, Cyanobacteria, Daphnia, and Zebrafish

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