Sequential application of electron donors and humic acids for the anaerobic bioremediation of chlorinated aliphatic hydrocarbons

Scherr, Kerstin E.; Nahold, Manfred; Lantschbauer, Wolfgang; Loibner, Andreas P.

doi:10.1016/j.nbt.2011.04.010

Cited by 16 publications

(9 citation statements)

References 57 publications

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“…Accordingly, humic acids are considered as electron shuttles in sediments in which kinetic oxidation and reduction processes are mediated by microbes (Kappler et al 2004). When humic acids are dissolved in water, even at a low concentration, the electron shuttle effect could occur (Jiang and Kappler 2008; Rowland et al 2007;Scherr et al 2011). Therefore, the results showed that the evident ferrous iron and arsenite released from sediments were enhanced by the addition of humic acids in the presence of microbes under anaerobic conditions (Fig.…”

Section: Effects Of Humic Acids On Ferrous Iron and Arsenite Mobilizamentioning

confidence: 97%

“…Therefore, the enhanced sedimentary ferrous iron and arsenite releases by microbes that are enhanced by humic acids under anaerobic conditions might be caused by multiple mechanisms, including bacterial respiration by using humic acids as electron acceptors , electron donors (Zhang et al 2014), the electron shuttle effect of humic acids (Jiang and Kappler 2008;Rowland et al 2007;Scherr et al 2011), and the microbially involved complexation process with organic matter ligands (Cesco et al 2000;Colombo et al 2014;Fortin and Langley 2005;Lemanceau et al 2009;Tipping and Hurley 1992).…”

Section: Microbial Community Analysis In Sediments Amended With Humicmentioning

confidence: 99%

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Humic acids enhance the microbially mediated release of sedimentary ferrous iron

Chang

Wei

Lin

et al. 2015

Environ Sci Pollut Res

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Iron (Fe) is an essential element for many organisms, but high concentrations of iron can be toxic. The complex relation between iron, arsenic (As), bacteria, and organic matter in sediments and groundwater is still an issue of environmental concern. The present study addresses the effects of humic acids and microorganisms on the mobilization of iron in sediments from an arsenic-affected area, and the microbial diversity was analyzed. The results showed that the addition of 50, 100, and 500 mg/L humic acids enhanced ferrous iron (Fe(II)) release in a time-dependent and dose-dependent fashion under anaerobic conditions. A significant increase in the soluble Fe(II) concentrations occurred in the aqueous phases of the samples during the first 2 weeks, and aqueous Fe(II) reached its maximum concentrations after 8 weeks at the following Fe(II) concentrations: 28.95 ± 1.16 mg/L (original non-sterilized sediments), 32.50 ± 0.71 mg/L (50 mg/L humic acid-amended, non-sterilized sediments), 37.50 ± 1.85 mg/L (100 mg/L humic acid-amended, non-sterilized sediments), and 39.00 ± 0.43 mg/L (500 mg/L humic acid-amended, non-sterilized sediments). These results suggest that humic acids can further enhance the microbially mediated release of sedimentary iron under anaerobic conditions. By contrast, very insignificant amounts of iron release were observed from sterilized sediments (the abiotic controls), even with the supplementation of humic acids under anaerobic incubation. In addition, the As(III) release was increased from 50 ± 10 μg/L (original non-sterilized sediments) to 110 ± 45 μg/L (100 mg/L humic acid-amended, non-sterilized sediments) after 8 weeks of anaerobic incubation. Furthermore, a microbial community analysis indicated that the predominant class was changed from Alphaproteobacteria to Deltaproteobacteria, and clearly increased populations of Geobacter sp., Paludibacter sp., and Methylophaga sp. were found after adding humic acids along with the increased release of iron and arsenic. Our findings provide evidence that humic acids can enhance the microbially mediated release of sedimentary ferrous iron in an arsenic-affected area. It is thus suggested that the control of anthropogenic humic acid use and entry into the environment is important for preventing the subsequent iron contamination in groundwater.

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Section: Effects Of Humic Acids On Ferrous Iron and Arsenite Mobilizamentioning

confidence: 97%

Section: Microbial Community Analysis In Sediments Amended With Humicmentioning

confidence: 99%

Humic acids enhance the microbially mediated release of sedimentary ferrous iron

Chang

Wei

Lin

et al. 2015

Environ Sci Pollut Res

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“…In summary, possible strategies to enhance anaerobic petroleum hydrocarbon degradation using naturally occurring, poorly soluble TEA include the addition of electron shuttling compounds (ES) of natural or anthropogenic origin, as was demonstrated for the anaerobic oxidation of dichloroethene (Scherr et al, 2011) before, to contaminated aquifers. The addition or targeted stimulation of microorganisms equipped with the metabolic capability to transfer electrons to mineral surfaces, via secretion of chelators and microbial ES or expression of conductive protein appendages, such as Geobacter or Shewanella, represents another approach.…”

Section: Potential Use Of Extracellular Electron Transfer In Bioremedmentioning

confidence: 99%

“…In many studies, anthraquinone disulfonate (AQDS) was used as a model humic substance (Aulenta et al, 2010;Bhushan et al, 2006;Collins and Picardal, 1999;Kwon and Finneran, 2006;Kwon and Finneran, 2008;Scherr et al, 2011) to study extracellular electron transfer in the reduction of halogenated and nitrous organic compounds.…”

Section: Role Of Quinones In Electron Shuttlingmentioning

confidence: 99%

Extracellular Electron Transfer in in situ Petroleum Hydrocarbon Bioremediation

Scherr¹

2013

Hydrocarbon

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“…El empleo de microorganismos en medios definidos en procesos de descontaminación es conocido, sin embargo las interacciones que suceden en ellos necesitan más estudios, por ejemplo el efecto de agregar nutrientes específicos a los ambientes mineros; aunque si existen experiencias agregando nutrientes para tratar compuestos tóxicos en otras actividades productivas (Atlas & Bartha 2002, Scherr et al 2011 El objetivo del presente trabajo fue obtener información relacionada al uso de nutrientes (lactato, acetato y ácido fosfórico) y microrganismos (BSR-BC) que pueden ser utilizados en procesos de biorremediación de relaves de cianuración. En una primera fase se usó un diseño factorial para optimizar de manera independiente la reducción de sulfatos y la degradación de cianuro procedente de un relave de cianuración, y en una segunda fase se intentó optimizar ambos procesos en conjunto.…”

Section: Introductionunclassified

Optimization of cyanidation tailings bioremediation adding nutrients and microorganisms

Hurtado

Berastain

2012

Rev peru biol

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The objective of this project was to find optimum parameters for the development of bioremediation process of cyanidation tailings by addition of nutrients and microorganisms. It was tested the use of sulfate-reducing bacteria (SRB) to stabilize metal ions through the formation of sulphides and; bacteria able to biodegrade cyanide (BC). Factorial experiments were conducted at level of columns. SRB from Cyanidation tailings were isolated and BC proceeded from pregnant solution of cyanidation. BSR and BC were adapted to tailings. A column test was performed to try: 1. the effect of using 1 and 10 mM lactate to increase the activity of BSR and 2. Addition of 0,1 and 1 mM sodium acetate and phosphoric acid to improve the activity of BC. In order to optimize parameters, it was tested in columns, effect of a mixed culture (SRB-BC) and nutrients (1 mM lactate and Acetate 1mM). Nutrients were found to increase the reduction of sulfate to 48% (average) and the addition of inoculums to 42%. Bioremediation of cyanide was 12%. It was not shown effect on the addition of nutrients or inoculums on Cyanide degradation. Two additional tailings were tested and the addition of 1 mM sodium lactate allowed the induction of BSR in 8 days in one of them.

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Sequential application of electron donors and humic acids for the anaerobic bioremediation of chlorinated aliphatic hydrocarbons

Cited by 16 publications

References 57 publications

Humic acids enhance the microbially mediated release of sedimentary ferrous iron

Humic acids enhance the microbially mediated release of sedimentary ferrous iron

Extracellular Electron Transfer in in situ Petroleum Hydrocarbon Bioremediation

Optimization of cyanidation tailings bioremediation adding nutrients and microorganisms

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