Sequencing of Chloroplast Genome of <i>Cucumis melo</i> L. var. <i>agrestis Naud.</i>

Abstract

Cucumis melo L. var. agrestis Naud. belong to the genus Cucumis in the Gourd family, it is rich in oil. It can extract high quality edible oil and provide raw materials for medicine and food industry. The chloroplast genome of Cucumis melo L. was sequenced for the first time. The results showed that the chloroplast genome of Cucumis melo L. was similar to that of common melon. This study can provide a theoretical reference for breeding.Keywords Cucumis melo L. var. agrestis Naud.; Chloroplast genome; Sequencing Field Muskmelon (Cucumis melo L. var. agrestis Naud.) belongs to the Cucumis of the family Cucurbitaceae, wild weeds. A number of scholars believe that the Field Muskmelon is the wild ancestor of Chinese oriental thin skin melon (Ma, 2020a). Seeds contain a lot of fat, which can be extracted from oil and eaten. Fruit rich in medicinal ingredients such as cucurbitacin, is a potential new type of wild plant (Ma, 2020b). At present, chloroplast genome sequencing fragments can be accurately assembled, but it's still difficult to assemble a larger complete genome, nuclear genome assembly is more difficult (Anton et al., 2012). Field Muskmelon is one of the most important cash crops in the world, Chloroplast genome sequencing has been successful, Field Muskmelon chloroplast genome contains 133 genes. The total GC content of the genome was 36.9% (Zhu et al., 2016). Genetic diversity of local muskmelon varieties in vietnam using chloroplast genome analysis (Phan et al., 2010). Genomic sequence polymorphisms were used to infer diversity and genetic differentiation of cultivated melon (Katsunori et al., 2013). And elaborated the melon chloroplast genome complete sequence and its systematic significance (Hong et al., 2020). So far, the study of chloroplast genome of Field Muskmelon has not been reported. In order to understand the composition of chloroplast genome and the relationship between genes and botanical traits, to provide theoretical and technical basis for breeding genetic improvement, The experiment material, Illumina high -throughput sequencing, The chloroplast genome sequencing of Field Muskmelon was completed.