Sequencing of a Urinary Stone Protein, Identical to α-1 Antitrypsin, Which Lacks 22 Amino Acids

Umekawa, Tohru; Kohri, Kenjiro; Amasaki, Naoya; Yamate, Takanori; Yoshida, K.; Yamamoto, Kazuhiko; Suzuki, Yasuyuki; Sinohara, Hyogo; Kurita, Takashi

doi:10.1006/bbrc.1993.1731

Cited by 21 publications

(7 citation statements)

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“…Boyce et al [13] and King et al [14] reported that the stone matrix is 64% protein, 5% proteoglycans, and 10% bound water. We reported the presence of calprotectin [15] and alpha-1-antitrypsin [16] in the matrix of calcium oxalate urinary stones. Acidic amino acids, such as aspartic acid and glutamic acid, are the major constituents of the matrix [17].…”

Section: Discussionmentioning

confidence: 99%

Interaction between Osteopontin on Madin Darby Canine Kidney Cell Membrane and Calcium Oxalate Crystal

et al. 1999

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We recently reported that the addition of the protein osteopontin (OPN) resulted in an increase in the deposition of calcium oxalate (CaOx) crystals on the surface of Madin Darby canine kidney (MDCK) cells. To determine the degree to which this increased deposition is caused by OPN, we investigated the extent to which the CaOx crystal deposition produced by the expression of OPN at the cell surface was suppressed by 4 different methods prior to the determination of the level of CaOx crystal binding. MDCK cells (2 × 10⁶ cells/well) were cultured to a confluent state, and the binding of OPN to the cellular surface was then inhibited by adding one of the following 4 substances: human OPN polyclonal antibody, thrombin, cyclic Arg-Gly-Asp (RGD) peptides and tunicamycin. The cells were cultured for 24 h. We then used a fluorescent antibody technique with an OPN polyclonal antibody to determined whether the expression of OPN at the cell surface was inhibited, and we measured the degree of CaOx crystal deposition using the isotope ⁴⁵Ca. The degree of CaOx crystal deposition was inhibited by 80% or more in the antibody-treated group, by 50–80% in the thrombin-treated group, by 60–80% in the cyclic RGD-treated group, and by 50–60% in the tunicamycin-treated group. These results suggest that OPN in the extracellular matrix is the main cause of CaOx crystal deposition on the surface of MDCK cells.

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Section: Discussionmentioning

confidence: 99%

Interaction between Osteopontin on Madin Darby Canine Kidney Cell Membrane and Calcium Oxalate Crystal

et al. 1999

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“…A slightly smaller species was also seen that most likely corresponded to posttranslational proteolytic processing that results in an N-terminal truncation. 19 The protein products from the rescued clones appeared to be identical to the protein produced from plasmid pCB-AT Zero that was used to generate the rAAV1-AAT vector. 13 Clone 302A-8 was predicted not to express protein based on sequence analysis, revealing the lack of an open reading frame because of complex rearrangements and recombination.…”

Section: Raav1-aat Clones Express Aatmentioning

confidence: 95%

Recombinant Adeno-Associated Virus Vector Genomes Take the Form of Long-Lived, Transcriptionally Competent Episomes in Human Muscle

Schnepp

Chulay

et al. 2016

Human Gene Therapy

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“…Various protein components have been identified as organic components involved in the mechanism of urinary stone formation. [5][6][7][8] OPN plays an important role during the formation of urinary calculi, 1,9,10 and its potent calcium-binding ability and cell-adhesive action make it a protein that is essential during the process of calcification in vivo. 11 OPN has also been shown to be a potent inhibitor of nucleation and of the growth of calcium oxalate crystal formation and the binding of calcium oxalate crystals to renal epithelial cells in vitro.…”

Section: Introductionmentioning

confidence: 99%

Urinary concentration of osteopontin and association with urinary supersaturation and crystal formation

et al. 2007

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Objective: In this study, we measured urinary osteopontin (OPN) concentrations in urolithiasis patients as well as in healthy volunteers, and investigated the relationship between urinary excretion of OPN and urinary supersaturation level. Methods: Supersaturation levels (AP indexes) were determined by using Tiselius's index. Crystals with a maximum diameter of 12 ìm or larger and less than 5 ìm were counted by scanning electron microscopy. A sum of cross-sectional areas of crystals was also calculated as the total crystal volume (VT). Results: Urinary OPN concentrations in the group with no urinary stone were significantly higher than that in the urolithiasis patients with a tendency toward stone enlargement. AP indexes were observed to be significantly higher in patients with stone enlargement, whereas urinary OPN concentrations bore no definite relation to the urinary supersaturation levels. VT and number of large crystals (12 ìm or larger) in patients with a tendency toward stone enlargement were higher than healthy volunteers, but no differences were found between the number of micro-crystal with the diameter of less than 5 ìm. On the basis of the plots of VT and OPN concentrations, regression analysis revealed that VT and log OPN had a significant correlation. Conclusion: Urinary OPN tended to be lower in cases with larger crystal volumes and is potentially associated with crystal growth for inhibitory effect.

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Sequencing of a Urinary Stone Protein, Identical to α-1 Antitrypsin, Which Lacks 22 Amino Acids

Cited by 21 publications

References 0 publications

Interaction between Osteopontin on Madin Darby Canine Kidney Cell Membrane and Calcium Oxalate Crystal

Interaction between Osteopontin on Madin Darby Canine Kidney Cell Membrane and Calcium Oxalate Crystal

Recombinant Adeno-Associated Virus Vector Genomes Take the Form of Long-Lived, Transcriptionally Competent Episomes in Human Muscle

Urinary concentration of osteopontin and association with urinary supersaturation and crystal formation

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