Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant<i> Luffa cylindrica</i>

Wang, Yahui; Liu, Xiaohong; Zhang, Rongrong; Yan, Zhiming; Xiong, Ai‐Sheng; Su, Xiaojun

doi:10.7717/peerj.9661

Cited by 5 publications

(3 citation statements)

References 64 publications

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“…LcCAT1 and LcCAT2 genes were significantly expressed on the second and third day, respectively, indicating that LcCAT1 and LcCAT2 regulated CAT enzyme activity at the transcriptional level (Figure 5f,g). Ya Wang et al sequenced the fresh-cut luffa with browning resistance and browning susceptibility and analyzed the expression of the LcCAT gene family, which also confirmed our results [66]. Zhu et al sequenced fresh-cut luffa and found that CAT enzyme activity was regulated by the LcCAT1 and LcCAT2 genes [2].…”

Section: Discussionsupporting

confidence: 81%

Effect of CaCl2 Treatment on Enzymatic Browning of Fresh-Cut Luffa (Luffa cylindrica)

Feng¹,

Cao

Wang³

et al. 2022

Horticulturae

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Enzymatic browning is a major issue that reduces the commercial value of Luffa cylindrica during storage, processing, and transportation. Our results showed that 1% CaCl2 treatment was optimal for reducing the surface browning of fresh-cut luffa. After storage at 25 °C for four days, the treated luffa had a significantly higher total phenolic (TP) content than the untreated luffa. At the end of the storage period, the calcium treatment showed low malondialdehyde (MDA) and hydrogen peroxide (H2O2) accumulation in the luffa. The treated luffa maintained higher superoxide dismutase (SOD), catalase (CAT), and phenylalanine ammonia lyase (PAL) activities and lower polyphenol oxidase (PPO) activity as compared to the untreated luffa. Furthermore, the genes regulating SOD (e.g., SOD1, SOD2, and SOD3), CAT (e.g., LcCAT1 and CAT2), and PAL (e.g., PAL1 and PAL2) in calcium-treated luffa were upregulated to varying degrees, suggesting that Ca2+ inhibited the browning of fresh-cut tissue by regulating the activities of those enzymes. Ultrastructure images showed that the treated luffa could maintain the relative integrity of the cell membrane and organelles. Therefore, Ca2+ might act as a second messenger to reduce ROS oxidative damage and maintain the cell membrane integrity. This study provides new insights into the breeding of new luffa varieties that are resistant to browning and post-harvest treatments to reduce the browning of luffa tissue.

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Section: Discussionsupporting

confidence: 81%

Effect of CaCl2 Treatment on Enzymatic Browning of Fresh-Cut Luffa (Luffa cylindrica)

Feng¹,

Cao

Wang³

et al. 2022

Horticulturae

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“…The WRKY transcription factor family, involved in ABA signaling pathway and stress signaling, is closely related to ROS production and scavenging (Chen et al, 2010; He, 2017). Also, WRKY transcription factors are involved in the fruit browning process by regulating the ROS level in Chinese walnut (Zhang et al, 2019), luffa (Wang et al, 2020), and apple (Zuo et al, 2021). The ROS level might decrease with the downregulation of WRKY60 expression during postharvest browning.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Nephelium lappaceum: Sodium nitroprusside‐induced delays of postharvest browning

Zhang,

Jiang,

et al. 2023

Physiologia Plantarum

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Sodium nitroprusside (SNP), as a nitric oxide donor, is widely used in postharvest fruit physiology and metabolism. Our previous study has indicated that SNP plays a crucial role in postharvest browning control of rambutan, but the molecular mechanism underlying this process is still unclear. In this research, we investigated the gene expression and function of postharvest rambutan in response to SNP during browning. We found 7336 differentially expressed genes (DEGs), among which 2206 were upregulated and 5130 were downregulated. Gene Ontology (GO) enrichment as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed, and the real-time quantitative PCR (qPCR) data were consistent with transcriptome data.The DEGs relevant to rambutan pericarp browning were mainly involved in anthocyanin biosynthesis, phenolic oxidation, reactive oxygen species (ROS) production, and energy supply. It was shown that SNP regulated the synthesis and degradation of anthocyanins, accumulation of phenols, level of ROS and energy metabolism to suppress the postharvest browning of rambutan. Also, one WRKY transcription factor involved in ROS metabolism was observed to be differentially regulated. These findings add to our insights into the molecular mechanisms of the SNP-induced browning delays of rambutan, which has implications for subsequent studies on molecular mechanisms of fruit browning.

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“…We used BLASTP (Gallin and Boutet, 2011) to retrieve 28 K + channel protein sequences from apple (Malus domestica) at NCBI (9 AKT, 4 KAT, 4 SKOR, and 11 two-pore domain potassium channel genes). Taking these 28 apple K + protein channel sequences as templates, we used Primer Premier 6.0 (Wang et al, 2020) to design specific primers and ultimately cloned 8 K + channel genes from 12-2 (Supplementary Table S1). With cDNA from 12-2 as a template, we used the high-fidelity enzyme 2 × Phanta Max Master Mix (Vazyme Biotech, Nanjing, Jiangsu, China) for PCR amplification.…”

Section: Cloning and Screening Of K + Channel Genes From 12-2mentioning

confidence: 99%

Homologous Cloning of Potassium Channel Genes From the Superior Apple Rootstock Line 12-2, Which is Tolerant to Apple Replant Disease

et al. 2022

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Potassium channels are important ion channels that are responsible for the absorption of potassium in the plant nutrient uptake system. In this study, we used homologous molecular cloning to obtain 8 K+ channel genes from the superior apple rootstock line 12-2 (self-named): MsAKT1-1, MsKAT3-2, MsKAT1-3, MsK2P3-4, MsK2P3-5, MsK2P5-6, MsK2P3-7, and MsK2P3-8. Their lengths varied from 942 bp (MsK2P5-6) to 2625 bp (MsAKT1-1), and the number of encoded amino acids varied from 314 (MsK2P5-6) to 874 (MsAKT1-1). Subcellular localization predictions showed that MsAKT1-1, MsKAT3-2, and MsKAT1-3 were localized on the plasma membrane, and MsK2P3-4, MsK2P3-5, MsK2P5-6, MsK2P3-7, and MsK2P3-8 were localized on the vacuole and plasma membrane. The 8 K+ channel proteins contained α helices, extended strands, β turns, and random coils. MsKAT1-3 had four transmembrane structures, MsKAT3-2 had six, and the other six K+ channel genes had five. Protein structure domain analysis showed that MsAKT1-1 contained nine protein domains, followed by MsKAT3-2 with four, MsKAT1-3 with three, and the other five two-pore domain K+ channel proteins with two. Semi-quantitative RT-PCR detection of the K+ channel genes showed that their expression levels were high in roots. qRT-PCR analysis showed that the relative expression levels of the 8 genes changed after exposure to ARD stress. The above results provide a theoretical basis for further research on the functions of potassium channel genes in 12-2 and a scientific basis for the breeding of ARD-resistant rootstock.

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Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica

Cited by 5 publications

References 64 publications

Effect of CaCl2 Treatment on Enzymatic Browning of Fresh-Cut Luffa (Luffa cylindrica)

Effect of CaCl2 Treatment on Enzymatic Browning of Fresh-Cut Luffa (Luffa cylindrica)

Transcriptome profiling of Nephelium lappaceum: Sodium nitroprusside‐induced delays of postharvest browning

Homologous Cloning of Potassium Channel Genes From the Superior Apple Rootstock Line 12-2, Which is Tolerant to Apple Replant Disease

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