Sequencing and immunochemical characterization of the American cockroach Per a 3 (CrPI) isoallergenic variants

Wu, Chen; Lee, Mey F.; Wang, Nancy M.; Luo, Shue Fen

doi:10.1016/s0161-5890(97)00009-6

Cited by 39 publications

(27 citation statements)

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“…Because tropomyosins from different cockroach species exhibit high amino acid sequence identity (>95%), rPer a 7 was also included (Table I). 7,[9][10][11][12][13][14][15][16][17][18][19][20][21] Previously, measurement of specific IgE antibodies to Bla g 2 by means of RIA was hindered by an inability to label this molecule with iodine 125. Given that titers of IgE antibodies to other cockroach allergens measured by means of RIA and streptavidin CAP assay were strongly correlated in initial studies (r > 0.8, P , .001), subsequent experiments used the streptavidin CAP assay for assessing patterns of IgE antibody responsiveness to all 5 cockroach allergens.…”

Section: Resultsmentioning

confidence: 99%

“…7,8 Rapid advances in the molecular characterization of cockroach allergens have resulted in the production of multiple recombinant allergens from German (Blattella germanica) and American (Periplaneta americana) cockroach species with comparable immunoreactivity to natural allergens. 7,[9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] However, a direct comparison of IgE and IgG antibody binding to different cockroach allergens has not been carried out among individuals with cockroach allergy, largely as a result of the lack of a reliable technique that could be applied to all allergens. Recent development of an assay that incorporates allergen bound to a high-capacity solid phase (streptavidin CAP 24,25 ) has facilitated definition of a hierarchy of cockroach allergens on the basis of IgE antibody binding.…”

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confidence: 99%

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Specific IgE and IgG antibody-binding patterns to recombinant cockroach allergens

Satinover

Reefer

Pomés

et al. 2005

Journal of Allergy and Clinical Immunology

111

133

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Specific IgE and IgG antibody-binding patterns to recombinant cockroach allergens

Satinover

Reefer

Pomés

et al. 2005

Journal of Allergy and Clinical Immunology

111

133

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“…Two hexamerins from the cockroach Periplaneta americana (Wu et al, 1996(Wu et al, , 1997; Accession Nos. AAB62731 and Q25641) are the closest homologs to Hex-1 (61% and 63% identity); however, the P. americana proteins do not contain hydrophobic tails.…”

Section: Hex-1 Structurementioning

confidence: 99%

Two hexamerin genes from the termite Reticulitermes flavipes: Sequence, expression, and proposed functions in caste regulation

Zhou

Tarver

Bennett

et al. 2006

Gene

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“…El cambio del aminoácido serina por prolina en el epítope de la isoforma Per a 3,0202 podría ser la causa de la menor reactividad de este isoalergeno, ya que dicho cambio parece alterar la estructura tri-dimensional disminuyendo su alergenicidad (43). Una minoría de los pacientes alérgicos al alergeno Am b a 1 presentan IgE específica casi exclusiva-mente hacia la isoforma Amb a I.1 (15).…”

Section: Alergenos Fuente No De Isoformas Técnicas De Identificaciónunclassified

El polimorfismo en los alergenos

2002

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Los alergenos son antígenos que inducen una respuesta inmunológica mediada por anticuerpos tipo IgE. Estos pueden provenir de diferentes fuentes como pólenes, ácaros, mohos, animales, insectos y alimentos. Una molécula alergénica consiste en un número determinado de epítopes o determinantes antigénicos. Algunos alergenos están constituidos por varias moléculas similares, las cuales presentan variaciones menores en la secuencia de aminoácidos, es decir, presentan polimorfismo y de acuerdo con el grado de similitud que presenten, se denominan isoalergenos o variantes. El polimorfismo puede ser el resultado de la presencia de varios alelos, como también de la presencia de familias de genes relacionados. Este fenómeno se ha detectado en alergenos de diferentes fuentes como los ácaros, los pólenes y las cucarachas, entre otros. El polimorfismo puede tener un efecto importante sobre los epítopes reconocidos por los linfocitos T, por los anticuerpos monoclonales y por la IgE de pacientes alérgicos, lo cual afecta la capacidad alergénica o el grado de reactividad cruzada con alergenos de otras especies. La existencia de isoformas con una capacidad nula o disminuida de unirse a la IgE, pero con capacidad de estimular los linfocitos T, se ha planteado como una alternativa atractiva en la inmunoterapia de las alergias. La estandarización de extractos alergénicos puede verse afectada por la presencia de isoformas en diferentes proporciones en fuentes alergénicas de diferentes regiones geográficas.Palabras clave: alergenos recombinantes, polimorfismo, isoalergenos, epítopes, IgE. Polymorphism in allergensAllergens are antigens that elicit an IgE-mediated immune response; they originate from diverse sources such as pollens, mites, molds, mammal exudates, insects and food. Allergenic molecules can contain several antigenic determinants, termed epitopes. Allergenic proteins have been discovered with polymorphisms, i.e., a mixture of similar molecules with minor variations in their amino acid sequences. These are called isoallergens or allergenic variants depending on the degree of similarity. Polymorphism may be defined by the presence of several alleles of the same gene or as families of related genes. Polymorphisms can have an important effect on the epitopes recognized by T lymphocytes, monoclonal antibodies and IgE of allergic patients. Individual polymorphisms can affect the basal level of allergenicity as well as the cross-reactivity with other allergens. The use of isoforms with low or total absence of IgE binding capacity but with high capacity to stimulate T cell response has been suggested as an alternative to the conventional immunotherapy for allergic diseases. Standardization of allergenic compounds can be affected by the differing proportions of isoforms in allergenic sources from different regions. En una fuente de alergenos, como los ácaros del polvo de habitación, se encuentran diversas proteínas con capacidad de unirse a la IgE. Los alergenos se caracterizan y diferencian básicamente por el tamaño molecular, e...

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Sequencing and immunochemical characterization of the American cockroach Per a 3 (CrPI) isoallergenic variants

Cited by 39 publications

References 36 publications

Specific IgE and IgG antibody-binding patterns to recombinant cockroach allergens

Specific IgE and IgG antibody-binding patterns to recombinant cockroach allergens

Two hexamerin genes from the termite Reticulitermes flavipes: Sequence, expression, and proposed functions in caste regulation

El polimorfismo en los alergenos

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