Sequencing and analysis of the complete mitochondrial genome ofOchotona coreanafrom China and its phylogenetic analysis

Abstract

The complete mitogenome sequence of Ochotona coreana was determined using long PCR. The genome was 17,283 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication, and 1 control region. The overall base composition of the heavy strand is A (31.5%), C (28.7%), T (26.2%), and G (13.6%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of O. coreana in Ochotonidae at the molecular level. The mitochondrial genome would be a significant supplement for the O. coreana genetic background. The seven Ochotona species formed a monophyletic group with the high bootstrap value (100%) in all examinations.