The complete mitogenome sequence of
Eothenomys eleusis
Thomas 1911 was determined using PCR. A circular double-stranded structure makes up the mitochondrial genome of
E. eleusis
. The complete length of the mitochondrial genome is 16,419 bp. The mitochondrial genome of
E. eleusis
included 13 protein-coding genes, 1 control region, 22 tRNA genes, 2 rRNA genes and 1 origin of L strand replication. The total base composition of
E. eleusis
mitochondrial genome was A (32.6%), T (26.3%), G (13.6%) and C (27.5%). We found significant A-T skew in base composition, especially in control regions and protein-coding genes.
E. eleusis
was supported by bootstrap values of 100%. This study verifies the evolutionary status of
E. eleusis
in Myodini tribe of Cricetidae at the molecular level. The mitochondrial genome would be a significant supplement for the
E. eleusis
genetic background.