Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response

Abstract

Background: Cassava, an allotetraploid known for its remarkable tolerance to abiotic stresses is an important source of energy for humans and animals and a raw material for many industrial processes. A full-length cDNA library of cassava plants under normal, heat, drought, aluminum and post harvest physiological deterioration conditions was built; 19968 clones were sequencecharacterized using expressed sequence tags (ESTs).

Differential Expression of Phenylalanine Ammonia-Lyase in Different Tissues of Sugarcane (Saccharum officinarum L.) during Development

“…Induction of PAL gene expression or increasing its enzyme activity by stress-related hormones and herbicides has been extensively reported (Jiang and Joyce 2003;Lee et al 2003;Panina et al 2005;Sakurai et al 2007;Ferrer et al 2008;Moura et al 2010;Jayalakshmi et al 2011;Jeong et al 2012;Koc and Ustun 2012).…”

Natural Diversity in Flowering Responses of Arabidopsis thaliana Caused by Variation in a Tandem Gene Array

“…Due to redundancy, the capacity to sustain nonlethal mutations is enhanced in gene families compared to singleton genes (Clark et al 2007;Armisen et al 2008). In plants, successful retention of new gene duplicates is biased toward genes that are involved in plant's ability to respond to environmental cues (Harrison and Gerstein 2002;Sakurai et al 2007;Korbel et al 2008). Pathogen response genes that belong to the nucleotide-binding site-leucine-rich repeat (NBS-LRR) gene family showcase functional diversification (Mondragon-Palomino et al 2002).…”

Transgenic Plants as a Tool for Plant Functional Genomics

“…Large sets of fl-cDNA clones have also been produced from several plants such as rice (Kikuchi et al 2003), wheat (Ogihara et al 2004), poplar (Nanjo et al 2007), soybean (Umezawa et al 2008), barley (K. Sato et al 2009), cassava (Sakurai et al 2007), sitka spruce (Ralph et al 2008), Physcomitrella patens (Nishiyama et al 2003), and Thellungiella halophila (Taji et al 2008).Well-characterized collections of cDNAs play an essential role in defining the function of genes and proteins in plants. The cDNA overexpression system is one of the approaches that use cDNA resources.…”