Sequences upstream and downstream of two xylem-specific pine genes influence their expression

No, Eun-Gyu; Zhou, Yuan; Loopstra, Carol A.

doi:10.1016/s0168-9452(00)00366-6

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…Some FLA proteins are associated with wood formation (No & Loopstra, 2000; Whetten et al ., 2001; Lorenz & Dean, 2002). In loblolly pine, two AGP coding genes, PtX3H6 and PtX14A9 , are up‐regulated in the radial expanding hypocotyls, probably upon hormone regulation (No & Loopstra, 2000; No et al ., 2000).…”

Section: Introductionmentioning

confidence: 99%

Poplar genes encoding fasciclin‐like arabinogalactan proteins are highly expressed in tension wood

Lafarguette

Leplé²,

Déjardin³

et al. 2004

New Phytologist

184

185

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Summary Fifteen poplar cDNA encoding fasciclin‐like arabinogalactan proteins (PopFLAs) were finely characterized, whereas the presence of arabinogalactan proteins (AGPs) was globally assessed during wood formation. PopFLAs transcript accumulation was analysed through EST distribution in cDNA libraries, semi‐quantitative RT‐PCR, microarray experiment and Northern blot analysis. Similarly, AGPs contents were globally quantified by rocket electrophoresis. AGPs accumulation was further examined by Western blotting and immunocytolocalization. Ten PopFLAs were specifically expressed in tension wood (TW) and not expressed in the cambial zone. Rocket electrophoresis revealed important AGPs accumulation in TW xylem. An anti‐AGPs specific antibody recognized two proteins preferentially present in the cell wall‐bound fraction from TW. Immunocytochemistry revealed a strong labelling close to the inner part of the G‐layer of TW fibres. PopFLAs are expressed in xylem and many are up‐regulated in TW. It is suggested that some PopFLAs accumulating at the inner side of the G‐layer may have a specific function in the building of this layer. PopFLAs expression may therefore be linked to the specific mechanical properties of TW.

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Section: Introductionmentioning

confidence: 99%

Poplar genes encoding fasciclin‐like arabinogalactan proteins are highly expressed in tension wood

Lafarguette

Leplé²,

Déjardin³

et al. 2004

New Phytologist

184

185

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“…1B ). Specific cleavage of the target site was confirmed by Sanger sequencing 26 . Non-specific cleaved fragments were also observed in the T7E1 assay, confirmed by Sanger sequencing to be SNPs in sheep ALPL that did not alter amino acid coding sequences (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Genetic engineering a large animal model of human hypophosphatasia in sheep

Williams

Pinzon

Huggins

et al. 2018

Sci Rep

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The availability of tools to accurately replicate the clinical phenotype of rare human diseases is a key step toward improved understanding of disease progression and the development of more effective therapeutics. We successfully generated the first large animal model of a rare human bone disease, hypophosphatasia (HPP) using CRISPR/Cas9 to introduce a single point mutation in the tissue nonspecific alkaline phosphatase (TNSALP) gene (ALPL) (1077 C > G) in sheep. HPP is a rare inherited disorder of mineral metabolism that affects bone and tooth development, and is associated with muscle weakness. Compared to wild-type (WT) controls, HPP sheep have reduced serum alkaline phosphatase activity, decreased tail vertebral bone size, and metaphyseal flaring, consistent with the mineralization deficits observed in human HPP patients. Computed tomography revealed short roots and thin dentin in incisors, and reduced mandibular bone in HPP vs. WT sheep, accurately replicating odonto-HPP. Skeletal muscle biopsies revealed aberrant fiber size and disorganized mitochondrial cristae structure in HPP vs. WT sheep. These genetically engineered sheep accurately phenocopy human HPP and provide a novel large animal platform for the longitudinal study of HPP progression, as well as other rare human bone diseases.

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“…The 5′‐untranslated region of the gene was functional as a promoter in both homologous and heterologous systems and appeared to contain regulatory sequences involved in transcriptional activation by light (Ávila et al 2001b). Furthermore, very recently the function of other gene promoters from conifers has also been characterized in heterologous systems (No et al 2000, Ciavatta et al 2002, Forward et al 2002). All these studies are contributing to a great extent to a better understanding of transcriptional regulation in this important group of trees.…”

Section: Introductionmentioning

confidence: 99%

Interaction of cis‐acting elements in the expression of a gene encoding cytosolic glutamine synthetase in pine seedlings

Gómez‐Maldonado

Ávila

Barnestein

et al. 2004

Physiologia Plantarum

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Glutamine synthetase (GS) genes, GS1a and GS1b, in pine (Pinus sylvestris L.) are differentially regulated in tissue specificity and during seedling development. To gain insight into the regulatory mechanisms controlling their expression, we have analysed the 5′‐flanking sequences of the gene GS1a using a transient expression system in pine protoplasts. Structural analysis of this region revealed the presence of putative regulatory elements including two AT‐rich elements and a poly CT consensus sequence. A series of 5′‐ and 3′‐deletions of the untranslated region covering the three putative elements, −800 to −626, −626 to −427 and +118 to +177 were analysed to demonstrate the functional implications of these elements in gene regulation. An electrophoretic mobility‐shift assay showed that nuclear proteins prepared from pine cotyledons interact with both AT‐rich regions (−800 to −427). Interestingly, no protein binding was detected when the untranslated region (+118 to +177) was included, even if deletion of that region suppressed promoter activity in the transient expression experiments conducted. However, simultaneous deletion of both types of cis elements, A/T and CT, resulted in a recovery of promoter activity of 50%. These results suggest a key regulatory role of the CT box by the interaction with A/T stretches in the distal part of the promoter and possibly with the proximal region (−427 to −1).

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Sequences upstream and downstream of two xylem-specific pine genes influence their expression

Cited by 10 publications

References 17 publications

Poplar genes encoding fasciclin‐like arabinogalactan proteins are highly expressed in tension wood

Poplar genes encoding fasciclin‐like arabinogalactan proteins are highly expressed in tension wood

Genetic engineering a large animal model of human hypophosphatasia in sheep

Interaction of cis‐acting elements in the expression of a gene encoding cytosolic glutamine synthetase in pine seedlings

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