Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco

Shirsat, A. H.; Wilford, Neville; Croy, R. R. D.; Boulter, D.

doi:10.1007/bf00339737

Cited by 180 publications

(96 citation statements)

References 26 publications

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“…1B). Several root tissue-specific cis-acting sequences (ATATT) were localized, as well as three CAAT box-like sequences at positions 27, 243, and 2323 that have been found in promoters of genes encoding seed storage protein (Shirsat et al, 1989). The ATTTTTA sequence is related Figure 1.…”

Section: Resultsmentioning

confidence: 99%

Localization in Roots and Flowers of Pea Chloroplastic Thioredoxin f and Thioredoxin m Proteins Reveals New Roles in Nonphotosynthetic Organs

et al. 2007

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Plant thioredoxins (TRXs) are involved in redox regulation of a wide variety processes and usually exhibit organ specificity. We report strong evidence that chloroplastic TRXs are localized in heterotrophic tissues and suggest some ways in which they might participate in several metabolic and developmental processes. The promoter regions of the chloroplastic f and m1 TRX genes were isolated from a pea (Pisum sativum) plant genomic bank. Histochemical staining for b-glucuronidase (GUS) in transgenic homozygous Arabidopsis (Arabidopsis thaliana) plants showed preferential expression of the 444-bp PsTRXf1 promoter in early seedlings, stems, leaves, and roots, as well as in flowers, stigma, pollen grains, and filaments. GUS activity under the control of the 1,874-bp PsTRXm1 promoter was restricted to the leaves, roots, seeds, and flowers. To gain insight into the translational regulation of these genes, a series of deletions of 5# elements in both TRX promoters were analyzed. The results revealed that a 126-bp construct of the PsTRXf2 promoter was unable to reproduce the expression pattern observed with the full promoter. The differences in expression and tissue specificity between PsTRXm1 and the deleted promoters PsTRXm2 and PsTRXm3 suggest the existence of upstream positive or negative regulatory regions that affect tissue specificity, sucrose metabolism, and light regulation. PsTRXm1 expression is finely regulated by light and possibly by other metabolic factors. In situ hybridization experiments confirmed new localizations of these chloroplastic TRX transcripts in vascular tissues and flowers, and therefore suggest possible new functions in heterotrophic tissues related to cell division, germination, and plant reproduction.

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Section: Resultsmentioning

confidence: 99%

Localization in Roots and Flowers of Pea Chloroplastic Thioredoxin f and Thioredoxin m Proteins Reveals New Roles in Nonphotosynthetic Organs

et al. 2007

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“…The results of the ciselements analysis confirmed that the functional diversities of the MtDof genes are mainly involved in tissue development, response to environmental stress, and interactions with other TFs. For example, motifs related to tissue development, including CAATBOX1 related to tissue-specific promoter activity (Shirsat et al, 1989), ROOTMOTIFTAPOX1 related to root development (Elmayan and Tepfer, 1995), POLLEN1LELAT52 and GTGANTG10 related to flower development (Rogers et al, 2001;Filichkin et al, 2004), and CACTFTPPCA1 related to leaf development (Gowik et al, 2004), were extensively present in the MtDof genes, and thus, most of these were highly expressed in roots, blades, buds, flowers, and seedpods. In addition, light responsive elements were also identified, such as GT1CONSENSUS (Terzaghi and Cashmore, 1995), and these were widely represented in the MtDof genes.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of the Dof gene family in Medicago truncatula

Shu

Zhang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The DNA-binding one zinc finger (Dof) family is a classic plant-specific zinc-finger transcription factor family, which is involved in many important processes, including seed maturation and germination, plant growth and development, and light responses. Investigation of the Medicago truncatula genome revealed 42 putative Dof genes, each of which holds one Dof domain. These genes were classified into four groups based on phylogenetic analysis, which are similar to the groups reported for Arabidopsis and rice. Based on genome duplication analysis, it was found that the MtDof genes were distributed on all chromosomes and had expanded through tandem gene duplication and segmental duplication events. Two main duplication regions were identified, one from tandem duplication and another from segmental duplication. By analyzing high-throughput sequencing data from M. truncatula, we found that most of the MtDof genes showed specific expression patterns in different tissues. According to cis-regulatory element analysis, these MtDof genes are regulated by different cis-acting motifs, which are important for the functional divergence of the MtDof genes in different processes. Thus, using genome-wide identification, evolution, and expression pattern analysis of the Dof genes in M. truncatula, our study provides valuable information for understanding the potential function of the Dof genes in regulating the growth and development of M. truncatula.

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“…affrc.go.jp/htdocs/PLACE/; Higo et al, 1999) indicated that the putative promoter contained four copies of the pollen-specific motifs (AGAAA) (POLLEN1LELAT52, Bate and Twell, 1998) and some other motifs associated with seed storage protein genes (CAATBOX1, Shirsat et al, 1989; EBOXBN-NAPA, Stalberg et al, 1996;and CANBNNAPA, Ellerstrom et al, 1996). To characterize tissue specificity of LeMAN5 expression, the 5Ј-upstream region of this gene (Ϫ543 to ϩ38) was cloned into a promoterless vector encoding a GUS gene to create a reporter vector.…”

Section: Characterization Of Leman5 Promoter In Arabidopsismentioning

confidence: 99%

A Novel Endo-β-Mannanase Gene in Tomato LeMAN5 Is Associated with Anther and Pollen Development

et al. 2004

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Endo-β-mannanase (EC 3.2.1.78) is involved in cell wall disassembly and the weakening of plant tissues by degrading mannan polymers in the cell walls. Endo-β-mannanase genes are expressed in tomato (Lycopersicon esculentum) seeds (LeMAN1 and LeMAN2) and fruits (LeMAN3 and LeMAN4). A novel endo-β-mannanase gene (termed LeMAN5) was found in the tomato genome by genome-walking PCR and bacterial artificial chromosome library screening. The 5′-upstream region of this endo-β-mannanase gene contained four copies of the pollen-specific cis-acting elements POLLEN1LELAT52 (AGAAA). A GUS-reporter gene driven with the putative LeMAN5 promoter (-543 to +38) was activated in anthers and pollen of transgenic Arabidopsis, with the highest β-glucuronidase activity detected in pollen. β-Glucuronidase expression was detected in mature pollen retained in sporangia, discharged pollen, and elongating pollen tubes in transgenic Arabidopsis. Consistently, expression of LeMAN5 mRNA and endo-β-mannnanase activity was detected in tomato anthers and pollen. In anthers, the highest mRNA expression and endo-β-mannanase activity were detected during late stages of anther development, when pollen maturation occurred. Endo-β-mannanase activity was present in discharged pollen, which was easily eluted in a buffer, indicating that the enzyme proteins are probably secreted from, and deposited on, the surface of pollen. These data suggest that the LeMAN5 endo-β-mannanase is associated with anther and pollen development.

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Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco

Cited by 180 publications

References 26 publications

Localization in Roots and Flowers of Pea Chloroplastic Thioredoxin f and Thioredoxin m Proteins Reveals New Roles in Nonphotosynthetic Organs

Localization in Roots and Flowers of Pea Chloroplastic Thioredoxin f and Thioredoxin m Proteins Reveals New Roles in Nonphotosynthetic Organs

Genome-wide identification and characterization of the Dof gene family in Medicago truncatula

A Novel Endo-β-Mannanase Gene in Tomato LeMAN5 Is Associated with Anther and Pollen Development

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