Sequences of three molluscan 5 S ribosomal RNAs confirm the validity of a dynamic secondary structure model

Abstract

The collection of known 5 S rRNA primary structures is enriched with the sequences from three mollusca, the snails Helix pomatia and Arion rufus, and the mussel Mytilus edulis. The three sequences can be fitted in a five-helix secondary structure model previously shown (De Wachter et al. (1982) Biochimie 64, 311-329) to apply to all 5 S RNAs regardless of their origin. One of the helices in this model can undergo a bulge-internal loop transition. Within the metazoan kingdom, the dimensions of each helix and loop are rigidly conserved, except for one helix which can comprise either 6 or 7 base pairs.

Molecular differentiation of the species of two squid families (Loliginidae and Ommastrephidae) based on a PCR study of the 5S rDNA gene

“…While the 5S rDNA gene has been used increasingly for the identification of species in recent years, its application to the study of mollusks has been limited primarily to bivalves (Cross, Rebordinos & Diaz 2006;Fang, De Baere, Vandenberghe & De Watcher, 1982). The present study was the first to apply the gene to the identification of cephalopods, and has proved that it can be a precise tool for the identification of species.…”

Sequence characterization and phylogenetic analysis of the 5S ribosomal DNA in some scallops (Bivalvia: Pectinidae)

“…In this species, the initial sequencing of three clones revealed the occurrence of units differing around 30 bp, which was confirmed by the sequence of two additional clones. Taking as reference the 5S rRNA sequences available in bivalves (Fang et al 1982; Stahl et al 1984), the coding region was assigned to 120 bp in the four cases and the spacer region to the remaining sequence (Table 1). BLAST analysis corroborated the identity of the sequence assigned to the 5S rRNA gene and indicated that the spacer region does not contain other well‐known sequences.…”

Cytogenetic characterization and mapping of rDNAs, core histone genes and telomeric sequences in Venerupis aurea and Tapes rhomboides (Bivalvia: Veneridae)

“…Universal primers were used to amplify both the whole internal transcribed spacer (ITS) region and a fragment of the 28S gene of the major rDNA repeats (ITS4, ITS5, LR10R, LR12; White et al 1990). For the 5S rDNA amplification, primers were designed from the sequence of the 5S rRNA of Mytilus edulis (Fang et al 1982). Amplification of the H2B/H2A and H3 core histone genes was performed using primers designed from the histone gene sequences of Mytilus edulis (Albig et al 2003) and those described by Giribet and Distel (2003), respectively.…”