Sequences involved in accurate and efficient transcription of human c-myc genes microinjected into frog oocytes.

Nishikura, Kazuko

doi:10.1128/mcb.6.11.4093

Cited by 25 publications

(26 citation statements)

References 44 publications

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“…Thus, even though the episomal constructs have established different nucleosomal structures upstream of the P2 promoter in different cell lines, all constructs showed similar extents of paused pol II at the P2 promoter. The finding that pol II can bind efficiently to the P2 promoter in MA76 cells in the presence of nucleosomes 3 and 4 suggests that sequences of the P2 core promoter extending from Ϫ70 to ϩ50 might be sufficient to bind pol II not only in vitro and in transient transfection experiments (20,43) but also when c-myc is stably transfected and assembled in chromatin. Within this region of the c-myc promoter, the ME1a1, ME1a2, and E2F transcription factor binding sites have been found (4,20,38,71).…”

Section: Discussionmentioning

confidence: 99%

Nucleosomal Structures of c-myc Promoters with Transcriptionally Engaged RNA Polymerase II

Albert

Mautner

Funk

et al. 1997

Molecular and Cellular Biology

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Organization of DNA into chromatin has been shown to contribute to a repressed state of gene transcription. Disruption of nucleosomal structure is observed in response to gene induction, suggesting a model in which RNA polymerase II (pol II) is recruited to the promoter upon reorganization of nucleosomes. Here we show that induction of c-myc transcription correlates with the disruption of two nucleosomes in the upstream promoter region. This nucleosomal disruption, however, is not necessary for the binding of pol II to the promoter. Transcriptionally engaged pol II complexes can be detected when the upstream chromatin is in a more closed configuration. Thus, upstream chromatin opening is suggested to affect activation of promoterbound pol II rather than entry of polymerases into the promoter. Interestingly, pol II complexes are detectable in both sense and antisense transcriptional directions, but only complexes in the sense direction respond to activation signals resulting in processive transcription.

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Section: Discussionmentioning

confidence: 99%

Nucleosomal Structures of c-myc Promoters with Transcriptionally Engaged RNA Polymerase II

Albert

Mautner

Funk

et al. 1997

Molecular and Cellular Biology

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“…Sequences between -60 and -37 relative to the human c-myc P1 start site are essential for its activity in microinjected Xenopus laevis oocytes (48). Sequences between -101 and -353 relative to P1 have been reported to possess both positive (37) and negative (19) effector activity on the human c-myc P1 and P2 start sites.…”

Section: Tcccctgtaccgccacatcgcgtacttggctggcaacttcgaagtcatcctgccagtcccmentioning

confidence: 99%

“…The sequence of MBP-1 does not contain any known DNA-binding motifs such as a zinc finger structure (25,43), leucine zipper (32), POU-specific regions (11,15,(64)(65)(66), or homeoboxes (33,40,42 Several groups have investigated the sequence requirement for initiation at the P1 and P2 start sites of the human c-myc gene (10,19,37,48,59). Sequences between -60 and -37 relative to the human c-myc P1 start site are essential for its activity in microinjected Xenopus laevis oocytes (48).…”

Section: Tcccctgtaccgccacatcgcgtacttggctggcaacttcgaagtcatcctgccagtcccmentioning

confidence: 99%

Cloning and characterization of a human c-myc promoter-binding protein.

Ray¹,

Miller²

1991

Mol. Cell. Biol.

107

128

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A human cDNA clone encoding a c-myc promoter-binding protein was detected by screening a HeLa cell k phage expression cDNA library. The library was screened by using an XhoI-NaeI human c-myc P2 promoter fragment as a probe. The recombinant phage encoded a fusion protein, myc-binding protein 1 (MBP-1), which had an apparent molecular size of 40 kDa. A corresponding protein with a molecular size of 35 kDa was present in a HeLa cell extract. Sequence analysis of the cloned gene reveals an open reading frame of 1,038 bp with a 3' untranslated region of 378 bp. The predicted protein sequence contains a proline-rich region in the amino terminus but does not demonstrate a known DNA-binding domain. DNase I footprint analysis demonstrates that MBP-1 binds to the sequence just 5' of the TATA box sequence of the human c-myc P2 promoter. MBP-1 cDNA hybridizes to a 1.4-kb mRNA from HeLa and HL-60 cells, indicating that the cDNA insert (1,416 bp) is a full-length clone. Coexpression of the MBP-1 protein represses transcription from the human c-myc promoter, suggesting that MBP-1 may act as a negative regulatory factor for the human c-myc gene.

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“…1). A recent report of microinjection experiments with MYC deletion constructs into frog oocytes suggested that Spl should bind to this particular sequence and mediate the observed transcriptional activity (Nishikura 1986). Using in vitro gel retardation assays, we could not obtain any evidence for the binding of Sp 1 to f-mycP2.…”

Section: Analogy In Binding Specificities Of the Myc Promoter Fragmenmentioning

confidence: 99%

Nuclear factor E2F mediates basic transcription and trans-activation by E1a of the human MYC promoter.

Thalmeier

Synovzik²,

Mertz³

et al. 1989

Genes Dev.

248

183

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Transcription from one of the two initiation sites, P1 and P2, of the dual human MYC promoter seems to be essential in all proliferating cells. To identify proteins and target structures for MYC regulation, a DNA region was analyzed that is critical for P2 promoter activity. Here, we show that a nuclear factor binds to a DNA element within P2, which is conserved perfectly between mouse and man and displays a striking homology to the Ela-inducible E2 promoter of adenovirus type 5 (AdS}. We demonstrate that the same transcription factor, defined recently as E2F, which plays an essential role in the activation of adenovirus early promoters and enhancers, also interacts as a dominant nuclear factor with the MYC promoter. The presence of an intact E2F binding site is required for basic expression and for trans-activation of the P2 promoter by Ela proteins. The human MYC promoter is the first cellular target described for E2F. The results suggest that expression of MYC might be regulated via modulation of E2F by cellular 'Ela-like' factors.

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Sequences involved in accurate and efficient transcription of human c-myc genes microinjected into frog oocytes.

Cited by 25 publications

References 44 publications

Nucleosomal Structures of c-myc Promoters with Transcriptionally Engaged RNA Polymerase II

Nucleosomal Structures of c-myc Promoters with Transcriptionally Engaged RNA Polymerase II

Cloning and characterization of a human c-myc promoter-binding protein.

Nuclear factor E2F mediates basic transcription and trans-activation by E1a of the human MYC promoter.

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