Sequences expressed sex-specifically in Drosophila melanogaster adults

DiBenedetto, Angela J.; Lakich, Delia; Kruger, Warren D.; Belote, John M.; Baker, Bruce S.; Wolfner, Mariana F.

doi:10.1016/0012-1606(87)90225-9

Cited by 73 publications

(39 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, the hierarchy must be acting, via dsx, to direct these tissues to an alternative developmental fate in the inappropriate sex (Hildreth, 1965;Keisman et al, 2001). Second, as shown here, dsx may also function in the appropriate sex in these three organs prior to adulthood, and probably during the late larval/early pupal period (DiBenedetto et al, 1987;Chapman and Wolfner, 1988;Feng et al, 1991), to establish the potential for the appropriate patterns of gene expression.…”

Section: On the Nature Of Sex-differential Gene Expression In Adultsmentioning

confidence: 87%

“…In the male accessory gland and ejaculatory duct, the sex hierarchy has been shown to function during the late larval/early pupal period to specify the adult expression patterns of several genes (DiBenedetto et al, 1987;Chapman and Wolfner, 1988;Feng et al, 1991). Our results for CG18284, CG17022 and CG17843, which are expressed exclusively in the male accessory glands, and for CG8708, which is expressed in the ejaculatory duct, are consistent with these findings in that the four genes are regulated by the action of the sex hierarchy prior to adulthood.…”

Section: On the Nature Of Sex-differential Gene Expression In Adultsmentioning

confidence: 99%

See 1 more Smart Citation

A genomic analysis ofDrosophilasomatic sexual differentiation and its regulation

et al. 2004

Self Cite

View full text Add to dashboard Cite

2008components of sex-differential expression were determined, but regulation by the sex-determination hierarchy was not explored.Here, we identify genes that are expressed sex-differentially in somatic tissues of adults and regulated by the sex hierarchy. Using arrays that assay approximately one-third (4040) of Drosophila genes, we analyzed adults mutant for the regulatory genes transformer (tra), dsx and fru (Fig. 1). To select a small number of such genes for further study, we chose a conservative approach. Stringent statistical analysis of these data, combined with data from wild-type adults and adults that lack germline tissue (Arbeitman et al., 2002), identified 63 genes that are sex-differentially expressed in the adult soma and regulated by the somatic sex hierarchy. Additional selection criteria, and validation by RNA blot analysis, defined a set of 11 genes for further characterization. In situ hybridization revealed that sex-differential expression of all 11 genes is confined to the internal genitalia. Analysis of the regulation of these genes revealed that the sex hierarchy functions during development to specify their adult expression patterns, and that dsx probably functions in diverse ways to set their activities. Materials and methods Drosophila stocks Microarray experimentsMicroarray production, data acquisition and analysis were performed as described in (Arbeitman et al., 2002). Each hybridization was a comparison of one RNA sample to a reference sample that was comprised of RNA derived from whole animals representing all stages of the life-cycle. Normalization was calculated so that the average ratio of signals from the experimental and reference sample equalled one. Analyses were performed on log-transformed ratio values. In all cases, chromosomal males and females were sampled separately. The wild-type data set and tudor data set were described previously (Arbeitman et al., 2002); each time point was sampled in duplicate. The wild-type data set includes 0-to 24-hour-, 3-day-, 5-day-, 10-day-, 15-day-, 20-day-, 25-day-and 30-day-old adults. The tudor data set includes 0-to 24-hour-and 5-day-old adults. Microarray experiments on sex determination mutants tra 1 /Df(3L)stj7 (XX), fru 4-40 /fru p1 (XY) and Dsx D /dsx m+r15 (XX) were performed with four (tra and fru) or five (Dsx D ) replicates. Microarray experiments performed on dsx null mutants (dsx m+r15 /dsx d+r3 ) were sampled in duplicate.

show abstract

Section: On the Nature Of Sex-differential Gene Expression In Adultsmentioning

confidence: 87%

Section: On the Nature Of Sex-differential Gene Expression In Adultsmentioning

confidence: 99%

A genomic analysis ofDrosophilasomatic sexual differentiation and its regulation

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…Early studies involving differential cDNA hybridization screens to identify male-specific RNAs expressed in accessory glands (Schäfer, 1986;DiBenedetto et al, 1987;Monsma and Wolfner, 1988;Simmerl et al, 1995;Wolfner et al, 1997), or (in one case) identification of an accessory gland peptide by a functional assay (Chen et al, 1988), identified 18 Acp genes; according to Drosophila convention, these genes are named 'Acp' followed by the designation of their chromosome position. SDS-PAGE analysis of accessory gland proteins coupled with statistical tests based on the frequency of 'multiple hits' in the cDNA screens suggested that there were about 50-100 different Acps (Chen, 1991;Wolfner et al, 1997).…”

Section: How Arementioning

confidence: 99%

“…Sequence classes of the 83 predicted Acps, isolated in several screens (Schäfer, 1986;DiBenedetto et al, 1987;Chen et al, 1988;Monsma and Wolfner, 1988;Simmerl et al, 1995;Wolfner et al, 1997;Swanson et al, 2001). The sequence and function of the four example Acps are described in: Acp26Aa (Monsma and Wolfner, 1988;Herndon and Wolfner, 1995;Heifetz et al, 2000;Chapman et al, 2001), Acp70A (Chen et al, 1988;Aigaki et al, 1991;Kubli, 1996;Moshitzky et al, 1996;Soller et al, 1997), Acp36DE Neubaum and Wolfner, 1999;Chapman et al, 2000) and Acp62F Lung et al, 2002).…”

Section: Figurementioning

confidence: 99%

The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila

2002

View full text Add to dashboard Cite

During mating, males transfer seminal proteins and peptides, along with sperm, to their mates. In Drosophila melanogaster, seminal proteins made in the male's accessory gland stimulate females' egg production and ovulation, reduce their receptivity to mating, mediate sperm storage, cause part of the survival cost of mating to females, and may protect reproductive tracts or gametes from microbial attack. The physiological functions of these proteins indicate that males provide their mates with molecules that initiate important reproductive responses in females. A new comprehensive EST screen, in conjunction with earlier screens, has identified ෂ90% of the predicted secreted accessory gland proteins (Acps). Most Acps are novel proteins and many appear to be secreted peptides or prohormones. Acps also include modification enzymes such as proteases and

show abstract

“…In in situ hybridization experiments, XDm4 hybridizes to polytene band 98C on the right arm of chromosome 3. Cross-hybridization experiments revealed that it is identical to the previously described clone mst336, which had been mapped to 98C as well and shown to hybridize to two RNAs of similar size (9). By sequence analysis, XDm4 was shown to contain a second gene of very similar sequence.…”

mentioning

confidence: 56%

Expression of a gene duplication encoding conserved sperm tail proteins is translationally regulated in Drosophila melanogaster.

Schäfer¹,

Börsch²,

Hülster³

et al. 1993

Mol. Cell. Biol.

View full text Add to dashboard Cite

We have analyzed a locus of Drosophila melanogaster located at 98C on chromosome 3, which contains two tandemly arranged genes, named Mst98Ca and Mst98Cb. They are two additional members of the Mst(3)CGP gene family by three criteria. (i) Both genes are exclusively transcribed in the male germ line. (ii) Both transcripts encode a protein with a high proportion of the repetitive motif Cys-Gly-Pro. (iii) Their expression is translationally controlled; while transcripts can be detected in diploid stages of spermatogenesis, association with polysomes can be shown only in haploid stages of sperm development. The genes differ markedly from the other members of the gene family in structure; they do not contain introns, they are of much larger size, and they have the Cys-Gly-Pro motifs clustered at the carboxy-terminal end of the encoded proteins. An antibody generated against the Mst98Ca protein recognizes both Mst98C proteins in D. melanogaster. In a male-sterile mutation in which spermiogenesis is blocked before individualization of sperm, both of these proteins are no longer synthesized. This finding provides proof of late translation for the Mst98C proteins and thereby independent proof of translational control of expression. Northern (RNA) and Western immunoblot analyses indicate the presence of homologous gene families in many other Drosophila species. The Mst98C proteins share sequence homology with proteins of the outer dense fibers in mammalian spermatozoa and can be localized to the sperm tail by immunofluorescence with an anti-Mst98Ca antibody.

show abstract

Sequences expressed sex-specifically in Drosophila melanogaster adults

Cited by 73 publications

References 34 publications

A genomic analysis ofDrosophilasomatic sexual differentiation and its regulation

A genomic analysis ofDrosophilasomatic sexual differentiation and its regulation

The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila

Expression of a gene duplication encoding conserved sperm tail proteins is translationally regulated in Drosophila melanogaster.

Contact Info

Product

Resources

About