Sequence variations of the bovine prion protein gene (<i>PRNP</i>) in native Korean Hanwoo cattle

Choi, Sang Ho; Woo, Hee Jong; Lee, Jeongmin

doi:10.4142/jvs.2012.13.2.127

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…For prnp genotyping, genomic DNA extracted from ear tissue (Wizard Ò Genomic DNA purification kit, Promega) was subjected to PCR with prnp specific primers to analyze the protein coding region according to Choi et al 30 and to determine the insertion-deletion variants in the promoter region (the 23 bp) and in intron 1 (12bp) as reported by Sander et al 24 . The purified PCR product of the prnp protein coding region was then submitted to automated cycle sequencing in both orientations.…”

mentioning

confidence: 99%

Identification of H-type BSE in Portugal

Orge

Machado

Ramalho³

et al. 2015

Prion

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mentioning

confidence: 99%

Identification of H-type BSE in Portugal

Orge

Machado

Ramalho³

et al. 2015

Prion

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“…A good approach to understanding the pathogenesis of prion disease may be through the analysis of chicken-specific amino acids in interspecies-conserved domains of prion proteins. Although insertion/deletion polymorphism in the octapeptide repeat domain of bovine prion protein has been reported in cattle, this polymorphism has never been associated with the susceptibility or resistance of prion disease [22–25, 47–49]. However, the variations of octapeptide repeat domain of human prion protein confer the susceptibility to prion disease in human [6, 31].…”

Section: Discussionmentioning

confidence: 99%

“…Approximately 40 SNPs of PRNP ORF have been reported in cattle. However, prion disease-related polymorphisms have not been reported thus far [22–25]. In addition, dog, which is known as prion disease resistant animal, showed very little polymorphisms in the PRNP ORF [26].…”

Section: Introductionmentioning

confidence: 99%

Absence of single nucleotide polymorphisms (SNPs) in the open reading frame (ORF) of the prion protein gene (PRNP) in a large sampling of various chicken breeds

2019

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BackgroundPrion diseases are zoonotic diseases with a broad infection spectrum among mammalian hosts and are caused by the misfolded prion protein (PrPSc) derived from the normal prion protein (PrPC), which encodes the prion protein gene (PRNP). Currently, although several prion disease-resistant animals have been reported, a high dose of prion agent inoculation triggers prion disease infection in these disease-resistant animals. However, in chickens, natural prion disease-infected cases have not been reported, and experimental challenges with prion agents have failed to cause infection. Unlike other prion disease-resistant animals, chickens have shown perfect resistance to prion disease thus far. Thus, investigation of the chicken PRNP gene could improve for understanding the mechanism of perfect prion-disease resistance. Here, we investigated the genetic characteristics of the open reading frame (ORF) of the chicken PRNP gene in a large sampling of various chicken breeds.ResultsWe found only tandem repeat deletion polymorphisms of the chicken PRNP ORF in the 4 chicken breeds including 106 Dekalb White, 100 Ross, 98 Ogolgye and 100 Korean native chickens. In addition, the distribution of chicken insertion/deletion polymorphisms was significantly different among the 4 chicken breeds. Finally, we found significant differences in the number of PRNP SNPs between prion disease-susceptible species and prion disease-resistant species. Notably, chickens lack SNPs in the ORF of the prion protein.ConclusionIn this study, we found that the absence of SNPs in the chicken PRNP ORF is a notable feature of animals with perfect resistant to prion disease.

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“…In another study with Korean cattle of the Hanwoo breed, the frequency for healthy animals was 42% (Choi et al 2012), whereas cattle from Poland exhibited a frequency of 36.3% (Gurgul et al 2012). The Turkish Gray breed exhibited a haplotype frequency (12ins.23ins) of 62%, which was higher than the frequency of healthy Korean and German cattle (Un et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms in the Prion Protein Gene of cattle breeds from Brazil

et al. 2016

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One of the alterations that occur in the PRNP gene in bovines is the insertion/deletion (indel) of base sequences in specific regions, such as indels of 12-base pairs (bp) in intron 1 and of 23- bp in the promoter region. The deletion allele of 23 bp is associated with susceptibility to bovine spongiform encephalopathy (BSE) as well as the presence of the deletion allele of 12 bp. In the present study, the variability of nucleotides in the promoter region and intron 1 of the PRNP gene was genotyped for the Angus, Canchim, Nellore and Simmental bovine breeds to identify the genotype profiles of resistance and/or susceptibility to BSE in each animal. Genomic DNA was extracted for amplification of the target regions of the PRNP gene using polymerase chain reaction (PCR) and specific primers. The PCR products were submitted to electrophoresis in agarose gel 3% and sequencing for genotyping. With the exception of the Angus breed, most breeds exhibited a higher frequency of deletion alleles for 12 bp and 23 bp in comparison to their respective insertion alleles for both regions. These results represent an important contribution to understanding the formation process of the Brazilian herd in relation to bovine PRNP gene polymorphisms.

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Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle

Cited by 5 publications

References 37 publications

Identification of H-type BSE in Portugal

Identification of H-type BSE in Portugal

Absence of single nucleotide polymorphisms (SNPs) in the open reading frame (ORF) of the prion protein gene (PRNP) in a large sampling of various chicken breeds

Polymorphisms in the Prion Protein Gene of cattle breeds from Brazil

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