Sequence variation of chalcone synthase gene in a spontaneous white-flower mutant of Chinese cabbage-pak-choi

Ming, Jun; Cao, Jiashu

doi:10.1007/s11033-007-9115-1

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…Truncated CHS3-ICHS1 is presented in mutant soybean (Glycine max) due to deletion at 5' flanking or coding region of ICHS1 in Ms-m mutant (Senda et al, 2002). Meanwhile, Jiang and Cao (2008) have observed nucleotide substitutions in BcCHS-wf at two positions (i.e., A to G at 37 and 970 bp, respectively) in both wild and mutant types of Chinese cabbage-Pak choi (Brassica campestris spp. chinensis).…”

Section: Discussionmentioning

confidence: 99%

“…The CHS gene families express enzymes that belong to type III polyketide synthases and are involved in flavonoid biosynthesis (Roslan, Huy, Kee et al, 2020;Roslan, Huy, Ming et al, 2020;Yuan et al, 2021). The CHS genes have been studied in many plants and showed up to 60% homologues sequences (Jiang & Cao, 2008). The CHS genes have been studied in Arabidopsis thaliana (Dao et al, 2011), Juglans regia (Cheniany et al, 2012), Oncidium Gower Ramsay (Liu et al, 2012), Malus domestica (Dare et al, 2013), Garbera hybrida (Deng et al, 2014), Triticum aestivum (Trojan et al, 2014), and some species of Zingiberaceae such as Curcuma longa (Ayer et al, 20108;Deepa et al, 2017;Resmi & Soniya, 2012), Boesenbergia rotunda (Chia et al, 2020;Roslan, Huy, Kee, et al, 2020;Roslan, Huy, Ming, et al, 2020), and Alpinia oxyphylla (Yuan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Preliminary in silico Analysis of CHS1 Gene in Commelinids Clade: Family Zingiberaceae, Costaceae, and Poaceae

Akram¹,

Zulkifly²,

Khamis³

et al. 2023

JTAS

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The chalcone synthase (CHS) gene families are known to be conserved in plants and have been well-studied in many plants, and they have an important role in the physiological and biological processes of plants. One of the studied CHS gene families is the CHS1 gene. CHS1 gene is known for its function in the flavonoid biosynthetic pathway. However, not many studies have been reported on the CHS1 gene in the Commelinids clade, especially the evolution of this gene within three families: Zingiberaceae, Costaceae, and Poaceae. Thus, this study aimed to perform a preliminary in silico comparative analysis of the CHS1 gene across these three families. Through this in silico comparative analysis, 20 partial sequences of the CHS1 gene, which are restricted to 565 bp regions, were analysed. The partial sequences were extracted from the National Center for Biotechnology Information database comprised of 16 Zingiberaceae species, three Costaceae species, and one Poaceae species. From the analysis, these targeted regions showed a low polymorphic site (18.23%) with 103 positions of single nucleotide polymorphisms and three mutations (substitution, insertion, and deletion). Meanwhile, phylogenetic analysis showed no clear evolutionary pattern within the three studied families. In conclusion, the studied partial sequences of the CHS1 gene in Zingiberaceae, Costaceae, and Poaceae showed that the gene is conserved within the Commelinids clade. Further studies to understand the consequences of low polymorphism and mutations as well as adaptive evolution in the CHS1 gene, accompanied by biochemistry and gene expression studies, should be done in these 20 species of Zingiberaceae, Costaceae, and Poaceae.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preliminary in silico Analysis of CHS1 Gene in Commelinids Clade: Family Zingiberaceae, Costaceae, and Poaceae

Akram¹,

Zulkifly²,

Khamis³

et al. 2023

JTAS

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“…CHS is also a key enzyme that catalyzes the formation of chalcone from malonyl CoA and 4-coumaryl CoA in anthocyanin biosynthesis [Li et al 2012, Guo 2013. Therefore, CHS affects plant flower color, and CHS alteration occurs based on whether CHS expression is increased or decreased [Jiang et al 2007]. For example, Tai et al [2014] introduced the CHS gene of Malus sinensis into tobacco, and the anthocyanin content increased in leaves with a positive correlation between anthocyanin content and CHS expression level.…”

Section: Discussionmentioning

confidence: 99%

The temporal changes of pigments content and key enzyme activities during autumnal turning period of Pistacia chinensis bunge

Wang,

Yang,

et al. 2023

Acta Sci. Pol. Hortorum Cultus

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In this study, the temporal regularity of pigments and key enzyme intermediates of Pistacia chinensis Bunge in the color-changing period was investigated to provide a theoretical basis for exploring the mechanism of leaf discoloration. The pigment content and activities of key enzymes of P. chinensis during leaf discoloration were investigated. The correlation between leaf discoloration and environmental factors (temperature, relative humidity, light) was also analyzed. During the color change, the chlorophyll content decreased, while no significant change in the carotenoid content was observed. The anthocyanin content significantly increased in the middle of the period of color change. The ratios of carotenoids/chlorophyll and anthocyanins/chlorophyll showed an upward trend during the period of color change. The lightness parameter (L) and hue parameter (B) of P. chinensis Bunge leaves showed a fluctuating tendency, reaching the highest value at the beginning of color conversion. The hue parameter (A) showed an upward trend at the color conversion stage. The 5-aminolevulinic acid (ALA) and porphobilinogen (PBG) values showed an upward-downward-upward trend. The contents of ALA and PBG at the end of the color transformation were 2.01 times and 2.88 times higher than those at the beginning. The activity of phenylalaninammo-nialyase increased during the color change period. Chalcone isomerase and chalcone synthase first increased, then declined, reaching their highest level in the middle of color conversion, 261.0 u/g and 157.3 u/g, respectively. Although the activities of both enzymes declined at the end of the color conversion, they were higher than at the beginning of the color change. Anthocyanin content was negatively correlated with temperature, relative humidity, day length, and chalcone isomerase, whereas it was positively correlated with phenylalaninammo-nialyase. The results revealed the reasons for the discoloration of P. chinensis leaves in autumn and thus should be considered when exploring the mechanism of color-changing plants and performing color-changing plant applications.

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Orychophragmus

Zhou¹,

Wu²,

Wang

2010

Wild Crop Relatives: Genomic and Breeding Resources

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Sequence variation of chalcone synthase gene in a spontaneous white-flower mutant of Chinese cabbage-pak-choi

Cited by 7 publications

References 26 publications

Preliminary in silico Analysis of CHS1 Gene in Commelinids Clade: Family Zingiberaceae, Costaceae, and Poaceae

Preliminary in silico Analysis of CHS1 Gene in Commelinids Clade: Family Zingiberaceae, Costaceae, and Poaceae

The temporal changes of pigments content and key enzyme activities during autumnal turning period of Pistacia chinensis bunge

Orychophragmus

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