Sequence variants and functional analysis of human papillomavirus type 16 E5 gene in clinical specimens

Hsieh, Chang‐Hsun; Tsao, Yeou‐Ping; Wang, C H; Han, Chih-Ping; Chang, Junn-Liang; Lee, J Y; Chen, S L

doi:10.1007/s007050070020

Cited by 22 publications

(14 citation statements)

References 22 publications

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“…Additionally, these findings support the idea that the E5 protein could be an early predictor marker of cervical cancer (Gill et al, 1998;Hsieh et al, 2000;Kell et al, 1994).…”

Section: E5 Functional Propertiessupporting

confidence: 81%

Molecular Bases of Human Papillomavirus Pathogenesis in the Development of Cervical Cancer

Pedroza-Saavedra¹,

Plett-Torres²,

Chihu-Amparán³

et al. 2012

Human Papillomavirus and Related Diseases - From Bench to Bedside - Research Aspects

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“…Additionally, these findings support the idea that the E5 protein could be an early predictor marker of cervical cancer (Gill et al, 1998;Hsieh et al, 2000;Kell et al, 1994).…”

Section: E5 Functional Propertiessupporting

confidence: 81%

Molecular Bases of Human Papillomavirus Pathogenesis in the Development of Cervical Cancer

Pedroza-Saavedra¹,

Plett-Torres²,

Chihu-Amparán³

et al. 2012

Human Papillomavirus and Related Diseases - From Bench to Bedside - Research Aspects

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“…E5 variations have been considered unrelated to malignant transformation. The mutational hotspots of A3979C, A4041G and A4076T in E5 were identified in our samples and have also been reported in Taiwan, Singapore, Brazil, Tanzania, Germany, and the USA [22,23,24]. The novel variants we found in L2 only caused synonymous amino acid changes.…”

Section: Resultsmentioning

confidence: 49%

Physical Status and Variant Analysis of Human Papillomavirus 16 in Women from Shanghai

Guo

Zhu

et al. 2015

Gynecol Obstet Invest

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Background/Aims: Human papillomavirus type 16 (HPV16) is the cause of more than half of all cases of cervical cancer. Genetic mutations in HPV16 and the integration of HPV16 DNA in the human genome are considered important genetic changes in cervical lesion progression. However, limited data concerning HPV16 lineages and physical integration status have been reported for Shanghai, China. The current study analyzed the genetic mutations in complete HPV16 genomes and the physical integration status of HPV16 DNA. Methods: A total of 30 samples of cervical exfoliated cells from patients with HPV16 infection were collected. The entire HPV16 genome was isolated, amplified by PCR and directly sequenced. The physical integration status was determined by 3′RACE nested PCR. Results: A total of 13 integration sites were identified, including 9 in common fragile sites and 1 not close to any fragile sites. Phylogenetic analysis identified two HPV lineages: the European (E) lineage and the East Asian (EA) lineage. Amino acid changes of D25E and N29S were the most common variations across the genome. The HPV16 early genes E1 and E7 and the late gene L1 tended to be highly conserved, whereas the early genes E2, E4 and E6 were more variable. Furthermore, 10 novel variations were identified in this study, which led to the 3 amino acid changes of S23I in E2 and E244K and T269I in E2/E4. Conclusion: Integrated HPV16 viruses were detected in all stages of cervical samples. Many variants in E2, E4, E7, and the long control region co-varied with E6 variations and helped to define the HPV16 lineages.

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“…This event could confer a selective growth advantage on such variants since it has been suggested that codon usage may be important in the regulation of early HPV expression. However, similar in vitro transforming activities for HPV-16 E5 variant proteins were also described (54).…”

Section: Nucleotide Variability and Oncogenic Potentialmentioning

confidence: 89%

Epidemiological and functional implications of molecular variants of human papillomavirus

Sichero¹,

Villa²

2006

Braz J Med Biol Res

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Human papillomavirus genomes are classified into molecular variants when they present more than 98% of similarity to the prototype sequence within the L1 gene. Comparative nucleotide sequence analyses of these viruses have elucidated some features of their phylogenetic relationship. In addition, human papillomavirus intratype variability has also been used as an important tool in epidemiological studies of viral transmission, persistence and progression to clinically relevant cervical lesions. Until the present, little has been published concerning the functional significance of molecular variants. It has been shown that nucleotide variability within the long control region leads to differences in the binding affinity of some cellular transcriptional factors and to the enhancement of the expression of E6 and E7 oncogenes. Furthermore, in vivo and in vitro studies revealed differences in E6 and E7 biochemical and biological properties among molecular variants. Nevertheless, further correlation with additional functional information is needed to evaluate the significance of genome intratypic variability. These results are also important for the development of vaccines and to determine the extent to which immunization with L1 virus-like particles of one variant could induce antibodies that cross-neutralize other variants. Key words Papillomavirus heterogeneityThe first evidence for the existence of different human papillomavirus (HPV) types was obtained in the early 1970's when it was observed that mRNA purified from plantar warts hybridized with DNA purified from other plantar warts but not from warts at other sites, including anogenital ones. In the following years, the genetic heterogeneity of HPV was confirmed by restriction fragment length polymorphism analysis and this was followed by an extensive and clear definition of HPV types based on DNA sequencing of different genes and the long control region (LCR) (1).HPV types are defined as a viral genome with an L1 late gene sequence that is at least 10% dissimilar from that of any other type. To date more than 120 different HPV genotypes associated with infections in humans have been described. In addition, the Papillomavirus Nomenclature Committee has established that HPV genomes be classified into molecular variants when they present more than 98% of similarity to the prototype

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Sequence variants and functional analysis of human papillomavirus type 16 E5 gene in clinical specimens

Cited by 22 publications

References 22 publications

Molecular Bases of Human Papillomavirus Pathogenesis in the Development of Cervical Cancer

Molecular Bases of Human Papillomavirus Pathogenesis in the Development of Cervical Cancer

Physical Status and Variant Analysis of Human Papillomavirus 16 in Women from Shanghai

Epidemiological and functional implications of molecular variants of human papillomavirus

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