Sequence Variability of Three Alleles of the Self-Incompatibility Gene of Nicotiana alata

Anderson, Marilyn A.; McFadden, Geoffrey I.; Bernatzky, Robert; Atkinson, Angela; Orpin, Timothy; Dedman, Helen; Tregear, Geoffrey W.; Fernley, Ross T.; Clarke, Adrienne E.

doi:10.2307/3868968

Cited by 73 publications

(129 citation statements)

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“…The isolated s6-glycoprotein preferentially inhibited S2 pollen on some occasions and s g pollen on others; it never preferentially inhibited S, pollen (Table 1). This inhibition by the s gglycoprotein may reflect homology in the mode of action of the Sp-and S6-glycoproteins; although the S,-and s6-glycoproteins are not more closely related in amino acid sequence than S2-and S3-or S3-and S6-glycoproteins (Anderson et al, 1989), the possibility of homology in functional regions is not precluded.…”

Section: Discussionmentioning

confidence: 99%

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Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata.

1989

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Section: Discussionmentioning

confidence: 99%

“…Pollen and styles were obtained from plants (Nicotiana alata Link et Otto) of the self-incompatible genotypes S&, S S 3 , and s6S6 from sources described in Anderson et al (1989).…”

Section: Plant Materialsmentioning

confidence: 99%

Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata.

1989

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“…(b) Comparisons of synonymous and non synonymous differences per site between SLG and the S-domain of SRK-alleles, within haplotypes and between different haplotypes. The regions of the two loci compared are indicated by the horizontal bars in the figure. strains of plants immediately revealed astonishing divergence between alleles for different incompatibility types, in both Solanaceae (Anderson et al, 1989) and Brassicaceae (Nasrallah et al, 1987). Both silent differences and multiple amino acid differences were found between S alleles.…”

Section: Quantitative Measures Of the Self-incompatibility Locus Polymentioning

confidence: 99%

“…charlesworth@ed.ac.uk ©1998 The Genetical Society of Great Britain. incompatibility types of plants in families, and encode sequences of cosegregating pistil proteins (Anderson et al, 1989;Li et al, 1995;Nasrallah et al, 1987;Walker et al, 1996). An important result from sequencing studies is that selfincompatibility loci have evolved from independent origins several times in flowering plants.…”

Section: Evolution and Maintenance Of Self-incompatibilitymentioning

confidence: 99%

Flowering plant self-incompatibility: the molecular population genetics of Brassica S-loci

Charlesworth

Awadalla

1998

Heredity

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Self-incompatibility systems in different angiosperm families are reviewed, and the evidence that incompatibility has arisen several times is outlined. New data on the sequence polymorphism of self-incompatibility loci from two different angiosperm families are compared with results from other highly polymorphic loci, particularly MHC loci. We discuss what molecular genetic analyses of these sequences can tell us about the nature and maintenance of the polymorphism at self-incompatibility loci. We suggest that there is evidence for recombination at the Brassica selfincompatibility loci, so that it may be possible to discern regions that are particularly functionally important in the recognition reaction, even though the long-term maintenance of polymorphisms in these amino acid residues has caused the evolution of many other sequence differences between alleles.

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“…These glycoproteins are very abundant in the extracellular matrix of the transmitting tract and are also expressed in the stigma and in the epidermis of the placenta (Cornish et Anderson et al, 1989;McClure et al, 1993). S RNases are essential for S allele-specific pollen rejection Murfett et al, 1994), and their ribonuclease activity is required for this function .…”

Section: Introductionmentioning

confidence: 99%

S RNase and Interspecific Pollen Rejection in the Genus Nicotiana: Multiple Pollen-Rejection Pathways Contribute to Unilateral Incompatibility between Self-Incompatible and Self-Compatible Species.

et al. 1996

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In self-incompatible (SI) plants, the S locus acts to prevent growth of self-pollen and thus promotes outcrossing within the species. lnterspecific crosses between SI and self-compatible (SC) species often show unilateral lncompatibility that follows the S I x SC rule: S I specles reject pollen from SC specles, but the reciproca1 crosses are usually compatible. The general validity of the S I x SC rule suggests a link between S I and interspeclflc pollen rejectlon; however, this link has been questioned because of a number of exceptlons to the rule. To clarlfy the role of the S locus in interspecific pollen rejection, we transformed severa1 Nicotlana species and hybrids with genes encodlng S A~ or SC,O RNase from S I N. alata. Compatibillty phenotypes in the transgenlc plants were tested using pollen from three SC specles showing unilateral incompatibility with N. alata. S RNase was lmpllcated ln rejecting pollen from all three specles. Rejection of N. plumbaginifolia pollen was similar to S allele-speclfic pollen rejection, showing a requirement for both S RNase and other genetic factors from N. alata. In contrast, S RNase-dependent rejectlon of N. glutinosa and N. tabacum pollen proceeded without these additional factors. N. alata also rejects pollen fmm the latter two specles through an S RNaseindependent mechanism. Our results lmplicate the S locus in all three systems, but lt 1s clear that multlple mechanisms contribute to interspecific pollen rejectlon. INTRODUCTIONMany plants have evolved genetically controlled selfincompatibility (SI) systems that promote outcrossing by restricting pollination between closely related individuals of the same species (de Nettancourt, 1977). Mechanisms also exist to restrict pollination between different species, but comparatively little is known about the control of interspecific pollination.As in other solanaceous plants, SI in the genus Nicotiana is controlled by a single multiallelic locus, the S locus (Newbigin et al., 1993). These plants employ a gametophytic SI system in which pollen is rejected if the S allele in the haploid pollen is the same as either S allele in the diploid pistil. S allele-specific pollen rejection occurs as pollen tubes grow through the extracellular matrix of the stylar transmitting tract (Newbigin et al., 1993). The products of the S locus in the style are the To whom correspondence should be addressed.S RNases (McClure et al., 1989). These glycoproteins are very abundant in the extracellular matrix of the transmitting tract and are also expressed in the stigma and in the epidermis of the placenta (Cornish et Anderson et al., 1989;McClure et al., 1993). S RNases are essential for S allele-specific pollen rejection Murfett et al., 1994), and their ribonuclease activity is required for this function . Following incompatible pollinations, RNA in selfpollen tubes is degraded, and this degradation is consistent with a cytotoxic model for pollen rejection (McClure et al., 1990;Gray et al., 1991; Dickinson, 1994). SI is therefore an active process i...

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Sequence Variability of Three Alleles of the Self-Incompatibility Gene of Nicotiana alata

Cited by 73 publications

References 6 publications

Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata.

Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata.

Flowering plant self-incompatibility: the molecular population genetics of Brassica S-loci

S RNase and Interspecific Pollen Rejection in the Genus Nicotiana: Multiple Pollen-Rejection Pathways Contribute to Unilateral Incompatibility between Self-Incompatible and Self-Compatible Species.

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