Sequence Typing Confirms that Campylobacter jejuni Strains Associated with Guillain-Barré and Miller-Fisher Syndromes Are of Diverse Genetic Lineage, Serotype, and Flagella Type

Abstract

Guillain-Barré syndrome (GBS) and Miller-Fisher syndrome (MFS) are correlated with prior infection byCampylobacter jejuni in up to 40% of cases. Nucleotide sequence-based typing of 25 C. jejuni isolates associated with neuropathy permitted robust comparisons with equivalent data from approximately 800 C. jejuni isolates not associated with neuropathy. A total of 13 genetic lineages and 20 flaA short variable region nucleotide sequences were present among the 25 isolates. A minority of isolates (4 of 25) had the flaA short variable region nucleotide sequences that were previously proposed as a marker for GBS-associated isolates. These 4 isolates probably represented the Penner serotype 19 lineage, which has been proposed to have an association with GBS.

Campylobacter sequence typing databases: applications and future prospects

“…This complex is associated with lipooligosaccharide (LOS) class B, which is thought to be a molecular mimic of gangliosides of human nerve cells (Kimoto et al, 2006;Mortensen et al, 2009). The ST-403 complex also accounted for one isolate in a European isolate collection, but this was consistent with its relative abundance amongst human disease isolates as a whole (Dingle et al, 2001a). It is uncertain whether the apparent greater prevalence of ST-403 amongst the Bangladesh GBS isolates truly represents an increased tendency to cause neuropathogenic disease, or whether it reflects a greater abundance amongst human disease isolates as a whole, similar to the distribution observed in Curaçao .…”

“…Two studies have highlighted the relative over-representation of the ST-22 complex in neuropathology-associated isolates (Dingle et al, 2001a;Nielsen et al, 2010): this complex also accounted for 2/10 of the GBS-associated isolates but none of 39 gastroenteritis isolates from Bangladesh. In addition the ST-403 complex, represented by two different STs, accounted for 5/10 GBS isolates as well as 26 % of gastroenteritis isolates from the same country (Islam et al, 2009).…”

“…These conditions are relatively rare, and well-described isolate collections of associated Campylobacter are small in number and contain relatively few isolates. Nevertheless, the application of sequence-based characterization has enabled comparative studies of the genotypes of isolates from uncomplicated gastroenteritis with data from various collections of Campylobacter isolates associated with GBS, MSF and reactive arthritis obtained in the Netherlands, Belgium, Denmark and Bangladesh (Dingle et al, 2001a;Islam et al, 2009;Nielsen et al, 2010). These studies show that the isolates from patients with neuropathology are diverse and broadly similar to those from gastroenteritis, supporting the suggestion that the genetic factors responsible for neuropathology are reassorted among C. jejuni strains by horizontal genetic exchange, made on the basis of hybridization studies of 1712 genes in 56 isolates associated with neuropathology (Taboada et al, 2007).…”

Prevalence of virulence and cytolethal distending toxin (CDT) genes in thermophilic Campylobacter spp. from dogs and humans in Gyeongnam and Busan, Korea

“…Recently Campylobacter enteritis has been identified as an important risk factor for the development of inflammatory bowel disease [13]. In addition, extra-intestinal Campylobacter-associated diseases are polyarthragia (reactive arthritis), Guillain-Barré syndrome (GBS), and Miller-Fisher syndrome [10].…”

Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

“…O MLST possui a vantagem de ser altamente reprodutível além do fato de que seus resultados podem ser disponibilizados em bancos de dados online, o que possibilita a análise comparativa da diversidade genética de bactérias isoladas em diferentes regiões do mundo (Maiden et al, 1998 Campylobacter spp., sendo considerada, atualmente, o método de tipagem "padrão ouro" para esse gênero (Dingle et al, 2001;Schouls et al, 2003;Ragimbeau et al, 2008;Asakura et al, 2012;Duarte et al, 2014). …”