Sequence-specific RNA Recognition by the Xenopus Y-box Proteins

Bouvet, Philippe; Matsumoto, Ken; Ap, Wolffe

doi:10.1074/jbc.270.47.28297

Cited by 132 publications

(22 citation statements)

References 36 publications

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“…Ybx1 contains a ‘cold shock’ domain (CSD; Figure 2—figure supplement 1A,B), similar to bacterial cold shock protein CspA (Eliseeva et al, 2012). Ybx1 homologs are associated with localized RNAs in Drosophila, Ciona, and Xenopus, and mutations in mouse Ybx1 cause lethality (Bouvet et al, 1995; Wilhelm et al, 2000; Tanaka et al, 2004; Eliseeva et al, 2012). Ybx1 contains a conserved actin binding domain (ABD), also found in Drosophila Ypsilon schachtel (Yps) (Figure 2—figure supplement 1A).…”

Section: Resultsmentioning

confidence: 99%

An essential role for maternal control of Nodal signaling

Kumari

Gilligan

Lim

et al. 2013

eLife

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Growth factor signaling is essential for pattern formation, growth, differentiation, and maintenance of stem cell pluripotency. Nodal-related signaling factors are required for axis formation and germ layer specification from sea urchins to mammals. Maternal transcripts of the zebrafish Nodal factor, Squint (Sqt), are localized to future embryonic dorsal. The mechanisms by which maternal sqt/nodal RNA is localized and regulated have been unclear. Here, we show that maternal control of Nodal signaling via the conserved Y box-binding protein 1 (Ybx1) is essential. We identified Ybx1 via a proteomic screen. Ybx1 recognizes the 3’ untranslated region (UTR) of sqt RNA and prevents premature translation and Sqt/Nodal signaling. Maternal-effect mutations in zebrafish ybx1 lead to deregulated Nodal signaling, gastrulation failure, and embryonic lethality. Implanted Nodal-coated beads phenocopy ybx1 mutant defects. Thus, Ybx1 prevents ectopic Nodal activity, revealing a new paradigm in the regulation of Nodal signaling, which is likely to be conserved.DOI: http://dx.doi.org/10.7554/eLife.00683.001

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Section: Resultsmentioning

confidence: 99%

An essential role for maternal control of Nodal signaling

Kumari

Gilligan

Lim

et al. 2013

eLife

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“…This was found to be contingent upon phosphorylation of the Ser-102 residue in the cold shock domain (CSD) implying that this domain is minimally required for centrosomal trafficking. Especially interesting is the fact that the CSD is necessary for binding oligonucleotides, including RNA due to the presence of two RNP motifs (Bouvet et al , 1995). As the centrosome contains an intrinsic compliment of RNA (Alliegro et al , 2006) it is possible that YB-1 is involved in regulating their translation.…”

Section: Discussionmentioning

confidence: 99%

YB-1 evokes susceptibility to cancer through cytokinesis failure, mitotic dysfunction and HER2 amplification

et al. 2011

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Y-box binding protein-1 (YB-1) expression in the mammary gland promotes breast carcinoma that demonstrates a high degree of genomic instability. In the present study, we developed a model of premalignancy to characterize the role of this gene during breast cancer initiation and early progression. Antibody microarray technology was used to ascertain global changes in signal transduction following the conditional expression of YB-1 in human mammary epithelial cells (HMEC). Cell cycle associated proteins were frequently altered with the most dramatic being LIM Kinase 1/2 (LIMK1/2). Consequently, the misexpression of LIMK1/2 was associated with cytokinesis failure that acted as a precursor to centrosome amplification. Detailed investigation revealed that YB-1 localized to the centrosome in a phosphorylation-dependent manner where it complexed with pericentrin and γ-tubulin. This was found to be essential in maintaining the structural integrity and microtubule nucleation capacity of the organelle. Prolonged exposure to YB-1 led to rampant acceleration toward tumourigenesis with the majority of cells acquiring numerical and structural chromosomal abnormalities. Slippage through the G1/S checkpoint due to overexpression of cyclin E promoted continued proliferation of these genomically compromised cells. As malignancy further progressed, we identified a subset of cells harbouring HER2 amplification. Our results recognize YB-1 as a cancer susceptibility gene with the capacity to prime cells for tumourigenesis.

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“…Nucleolin, on the other hand, while a well-established RBP, is tethered to the 52-be/protein complex via protein/protein interactions. YB1 is a member of the highly conserved cold shock domain family of proteins and is a multifunctional nucleic acid binding protein implicated in transcriptional activation and repression, cellular and viral mRNA binding, mRNA stabilization and translational control (Ansari et al, 1999; Bouvet et al, 1995; Capowski et al, 2001; Chen et al, 2000; Didier et al, 1988; Ranjan et al, 1993; Stenina et al, 2000). The autoantigen La is a well-characterized RBP that has been shown to stabilize histone mRNA (McLaren et al, 1997).…”

Section: Rna Binding Proteins That Interact With App Mrnamentioning

confidence: 99%

The regulation of AβPP expression by RNA-binding proteins

Westmark¹,

Malter

2012

Ageing Research Reviews

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Amyloid β-protein precursor (AβPP) is cleaved by β- and γ-secretases to liberate amyloid beta (Aβ), the predominant protein found in the senile plaques associated with Alzheimer’s disease (AD) and Down syndrome (Masters et al., 1985). Intense investigation by the scientific community has centered on understanding the molecular pathways that underlie the production and accumulation of Aβ Therapeutics that reduce the levels of this tenacious, plaque-promoting peptide may reduce the ongoing neural dysfunction and neuronal degeneration that occurs so profoundly in AD. AβPP and Aβ production are highly complex and involve still to be elucidated combinations of transcriptional, post-transcriptional, translational and post-translational events that mediate the production, processing and clearance of these proteins. Research in our laboratory for the past two decades has focused on the role of RNA binding proteins (RBPs) in mediating the post-transcriptional as well as translational regulation of APP messenger RNA (mRNA). This review article summarizes our findings, as well as those from other laboratories, describing the identification of regulatory RBPs, where and under what conditions they interact with APP mRNA and how those interactions control AβPP and Aβ synthesis.

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Sequence-specific RNA Recognition by the Xenopus Y-box Proteins

Cited by 132 publications

References 36 publications

An essential role for maternal control of Nodal signaling

An essential role for maternal control of Nodal signaling

YB-1 evokes susceptibility to cancer through cytokinesis failure, mitotic dysfunction and HER2 amplification

The regulation of AβPP expression by RNA-binding proteins

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