Sequence-Specific Recognition of Double-Stranded Nucleic Acids by λ Exonuclease-Phosphorylated DNA Mediated Target Interrogation
Xin Su,
Shengnan Fu,
Junjie Li
et al.
Abstract:The CRISPR-Cas system, guided by an RNA molecule, cleaves double-stranded DNA targets in proximity to a protospacer adjacent motif (PAM), enabling the applications in genetic editing/interference and molecular diagnostics1–3. However, the reliance on PAM-dependent recognition and cleavage has limited the range of targetable sequences and led to undesired off-target effects, resulting in false activations4–7. Here, we conducted single-molecule FRET (smFRET) analysis and discovered a remarkable enzyme activity o… Show more
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