Sequence-related amplified polymorphism and inter-simple sequence repeat marker-based genetic diversity and nuclear DNA content variation in common vetch (Vicia sativaL.)

Abstract

Genetic diversity of 30 common vetch (Vicia sativaL.) lines and cultivars obtained from various resources or collected from natural flora of Turkey was evaluated by using 55 sequence-related amplified polymorphism (SRAP) and five inter-simple sequence repeat (ISSR) primer sets, and their nuclear DNA contents were determined by flow cytometer. A total of 188 polymorphic loci were detected, with an average of 3.62 loci per primer. The percentage of polymorphic loci was 82.1%. The polymorphism information content values ranged from 0.12 to 0.96, with an average of 0.63. The genetic distance coefficients were in the range of 0.112–0.627. Cluster analysis revealed that the 27 lines and three cultivars could be divided into two main groups. No polyploidy was detected within any vetch lines tested while significant (P< 0.0001) nuclear DNA content differences were determined. The present study revealed that fast and accurate fingerprinting analysis could be done using SRAP and ISSR markers, which indicated existing significant variation among common vetch lines and cultivars.

Genetic and nuclear DNA content variation of stevia (Stevia rebaudiana Bertoni) accessions grown in Turkey

“…The suspensions were then transferred into a glass tube through 30 μl Cell Tris filter before the samples were incubated for 1 hour at 37 o C in 2000 μl of staining buffer for running in flow cytometer. The nuclear DNA contents of samples were calculated by using G1 peak means of sample and internal standart (Cil and Tiryaki, 2016). Three biological replications of each accession were used in the study.…”

Diversity Analysis of Common Vetch (Vicia Sativa L.) Lines and Cultivars Using Pairwise Combinations of Universal Rice Primers

“…As a complex taxon, V. sativa represents a diverse phylogenetic relationship [15,16]. Various type of molecular markers have been previously used to resolve inter-and intra-specific diversity in common vetch by using random amplified polymorphic DNA (RAPD) [15], sequence-related amplified polymorphism (SRAP) and inter-simple sequence repeat (ISSR) [17], amplified fragment length polymorphism (AFLP) [18], simple sequence repeats (SSRs) [19], expressed sequence tag-simple sequence repeat (EST-SSR) [20], cDNA-simple sequence repeat (cDNA-SSR) [21,22], start codon targeted polymorphism (SCoT) [23] and single nucleotide polymorphisms (SNPs) [24,25]. The recent advancements in plant biotechnology also resulted in avalanche of information in terms of DNA sequences and functional gene determination in various plant species [20,21,23,26].…”