Sequence rearrangement and duplication of double stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and Escherichia coli DNA polymerase I

Fagan, John B.; Pastan, Ira; Crombrugghe, Benoít de

doi:10.1093/nar/8.13.3055

Cited by 43 publications

(27 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5). Northern transfers of RNAs from phenotypically normal chondrocytes displayed a low level of the 8,800-base fibronectin RNA, detected by hybridization with the 32P-labeled fibronectin cDNA clone, pFN600 (14). Transformed chondrocytes showed a fourfold increase in fibronectin RNAs, to a level roughly comparable to that found in phenotypically normal skin fibroblasts.…”

mentioning

confidence: 86%

“…The following probes were used in the Northern hybridizations: pFN600, a 600-base-pair fibronectin cDNA (14); pCOL3, an 800-base-pair al(I) collagen cDNA (64); pCg45, a 2,500-base-pair a2(I) collagen cDNA (24); pCgII-12, a 740-base-pair al(II) collagen cDNA (28); and pHrB, a human 18S ribosomal DNA (59). The plasmids pFN600, pCg45, pCgII-12, and pHrB were generous gifts of K. Yamada RNAs from normal and transformed chondrocytes were subjected to Northern hybridization analysis with 32P-labeled pCgII-12, a cloned type II collagen cDNA (28) (Fig.…”

mentioning

confidence: 99%

“…In contrast, trans- Northern hybridization analysis of fibronectin RNAs. Total cellular RNAs from normal (N) and transformed (T) skin fibroblasts and vertebral chondrocytes were denatured by glyoxalation, separated by electrophoresis on 1% agarose gels, transferred to Gene Screen, and hybridized with the fibronectin cDNA pFN600 (14). In this experiment, all cells were infected with ts-RSV and grown at the nonpermissive (N) or permissive (T) temperature for transformation.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Control of types I and II collagen and fibronectin gene expression in chondrocytes delineated by viral transformation.

Allebach¹,

Boettiger²,

Pacifici³

et al. 1985

Mol. Cell. Biol.

View full text Add to dashboard Cite

We have analyzed the effects of transformation by Rous sarcoma virus on expression of types I and II collagen and fibronectin genes in vertebral chondrocytes and compared them with expression of these genes in skin fibroblasts. Transformed chondrocytes display a dramatically decreased amount of type II collagen RNA, which can account fully for the decreased synthetic rate of this protein. Paradoxically, these cells also display greatly increased amounts of type I collagen RNAs, which are translated efficientlv in vitro, but not in the intact cells. We show here that the type I collagen RNAs in transformed chondrocytes are nearly indistinguishable from those found in skin fibroblasts, and that they clearly differ from the type I collagen RNAs found in normal chondrocytes. Transformed chondrocytes also display an increased amount of fibronectin RNA, which can account fully for the increased synthetic rate of this protein. Thus, the effects of transformation by Rous sarcoma virus on type I collagen and fibronectin RNAs in chondrocytes are the opposite of those observed in fibroblasts, which display decreased amounts of these three RNAs. These data indicate that the effects of transformation on the genes encoding type I collagen and fibronectin must be modulated by host cell-specific factors. They also imply that the types I and II collagen genes may be regulated by different mechanisms, the type I genes being controlled at both transcriptional and posttranscriptional levels, and the type II gene being controlled primarily at the transcriptional level.Transformation of differentiated cells by tumor viruses has provided a useful tool for analyzing the mechanisms of cellular gene expression. This has been particularly true for the genes encoding extracellular matrix proteins, whose expression is decreased by transformation with certain viruses. For example, transformation of chicken embryo fibroblasts by Rous sarcoma virus (RSV) results in reduced synthesis of type I collagen (10,20,25,(45)(46)(47) and, in some cases, fibronectin (36,39). Similarly, transformation of chicken vertebral chondrocytes by RSV results in decreased synthesis of type II collagen and the major cartilage proteoglycan (2,18,37,38). The reduced rates of synthesis of type I collagen and fibronectin in transformed fibroblasts reflect decreased amounts of the corresponding RNAs (1, 3. 15,19,43,45,46,51) These data demonstrate that transformation by RSV has opposite effects on the expression of type I collagen and fibronectin genes in chondrocytes and fibroblasts and imply that the action of src is modulated by host cell factors that differ between these cell types. They also imply that the types I and II collagen genes may be regulated by different mechanisms. The genes encoding type I collagen, a protein which is synthesized by many cell types, appear to be regulated at both transcriptional and posttranscriptional levels. In contrast, the type II collagen gene, which is expressed only by a very limited number of terminally differentiated cell type...

show abstract

mentioning

confidence: 86%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Control of types I and II collagen and fibronectin gene expression in chondrocytes delineated by viral transformation.

Allebach¹,

Boettiger²,

Pacifici³

et al. 1985

Mol. Cell. Biol.

View full text Add to dashboard Cite

show abstract

“…These clones contained 3' noncoding regions homologous to pc hor2-1 but in the vicinity of the stop codons nucleotide sequences were obtained which represented inversions of the carboxy-terminal portion of the B1 hordein polypeptide code. Such inversions have been described as accidents occurring during reverse transcription and second strand synthesis (18,49,50). The interpretable sequences of these two clones were identical to those of pc hot2-1 and pc hor2-3.…”

Section: Nucleotide Sequence Heterogeneity Of Cdna Clonesmentioning

confidence: 99%

Nucleotide sequences of cDNA clones for B1 hordein polypeptides

Rasmussen

Hopp

Brandt

1983

Carlsberg Res. Commun.

View full text Add to dashboard Cite

“…The most plausible explanation for our failure to recover full-length cDNAs is that the long 5' leader of the mRNA, which is composed largely of A and U residues that are frequently present in homopolymer tracts, led to premature termination or copying artifacts. Problems associated with accurate copying of mRNA are well known and may be exacerbated when transcribing A+U-rich sequences (14,19,57).…”

Section: Resultsmentioning

confidence: 99%

The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs.

Podgorski¹,

Franke²,

Faure³

et al. 1989

Mol. Cell. Biol.

View full text Add to dashboard Cite

The cyclic nucleotide phosphodiesterase (phosphodiesterase) gene plays essential roles in the development of Dictyostelium discoideum during cellular aggregation and postaggregation morphogenesis. Genomic clones spanning the gene were isolated and used to determine the sequence and structure of the phosphodiesterase gene. We found an unusually complex organization for a gene of D. discoideum. Two transcripts of 2.4 and 1.9 kilobases (kb) were synthesized from start sites separated by 1.1 kb. A developmentally regulated promoter was utilized for the 2.4-kb mRNA, and a constitutive promoter regulated synthesis of the 1.9-kb transcript. The gene was found to be divided into four exons that are alternately spliced to give rise to the two mRNAs. The precursor of the 2.4-kb mRNA contained a 2.3-kb intron, whereas the precursor of the constitutive transcript was synthesized with a 1.7-kb intron. The two transcripts contained identical protein-coding regions and 400-nucleotide 3' untranslated sequences. The 2.4-kb developmentally regulated mRNA was distinguished by a long 5' untranslated leader of 666 nucleotides. The complex structure of the gene may allow multiple levels of control of the expression of the phosphodiesterase during development.

show abstract

Sequence rearrangement and duplication of double stranded fibronectin cDNA probably occurring during cDNA synthesis by AMV reverse transcriptase and Escherichia coli DNA polymerase I

Cited by 43 publications

References 16 publications

Control of types I and II collagen and fibronectin gene expression in chondrocytes delineated by viral transformation.

Control of types I and II collagen and fibronectin gene expression in chondrocytes delineated by viral transformation.

Nucleotide sequences of cDNA clones for B1 hordein polypeptides

The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs.

Contact Info

Product

Resources

About