Sequence-ready 1-Mb YAC, BAC and Cosmid Contigs Covering the Distal Imprinted Region of Mouse Chromosome 7

Kato, Reiko; Shirohzu, Hisao; Yokomine, Takaaki; Mizuno, Shin Ichi; Mukai, Tsunehiro; Sasaki, Hidetada

doi:10.1093/dnares/6.6.401

Cited by 10 publications

(7 citation statements)

References 13 publications

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“…(Right) Map of the region of MMU7 in conserved synteny to the BWS-associated region of human 11p15. Gene locations were taken from NCBI online sequencing data ( http://www.ncbi.nlm.nih.gov/cgi-bin/Entrez/maps.cgi?org=hum&chr=11 ), and from published maps ( Alders et al, 1997 ; Hu et al, 1997 ; Reid et al, 1997 ; Buettner et al, 1998 ; Bepler et al, 1999 ; Kato et al, 1999 ; Lee et al, 1999 ; Engemann et al, 2000 ; Onyango et al, 2000 ; Paulsen et al, 2000 ).…”

Section: Resultsmentioning

confidence: 99%

Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH

Mahy

Perry

Bickmore

2002

The Journal of Cell Biology

263

231

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Genes can be transcribed from within chromosome territories; however, the major histocompatibilty complex locus has been reported extending away from chromosome territories, and the incidence of this correlates with transcription from the region. A similar result has been seen for the epidermal differentiation complex region of chromosome 1. These data suggested that chromatin decondensation away from the surface of chromosome territories may result from, and/or may facilitate, transcription of densely packed genes subject to coordinate regulation.To investigate whether localization outside of the visible confines of chromosome territories can also occur for regions that are not coordinately regulated, we have examined the spatial organization of human 11p15.5 and the syntenic region on mouse chromosome 7. This region is gene rich but its genes are not coordinately expressed, rather overall high levels of transcription occur in several cell types. We found that chromatin from 11p15.5 frequently extends away from the chromosome 11 territory. Localization outside of territories was also detected for other regions of high gene density and high levels of transcription. This is shown to be partly dependent on ongoing transcription. We suggest that local gene density and transcription, rather than the activity of individual genes, influences the organization of chromosomes in the nucleus.

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Section: Resultsmentioning

confidence: 99%

Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH

Mahy

Perry

Bickmore

2002

The Journal of Cell Biology

263

231

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“…1; refs. [3][4][5][6][7][8]. In particular, CDKN1C is maternally expressed, although a faint paternal allele expression is observed in most human tissues (9).…”

Section: Structure and Regulation Of The Cdkn1c Genementioning

confidence: 99%

p57Kip2 and Cancer: Time for a Critical Appraisal

Borriello

Caldarelli

Bencivenga

et al. 2011

Molecular Cancer Research

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p57Kip2 is a cyclin-dependent kinase inhibitor belonging to the Cip/Kip family, which also includes p21Cip1 and p27Kip1. So far, p57Kip2 is the least-studied Cip/Kip protein, and for a long time its relevance has been related mainly to its unique role in embryogenesis. Moreover, genetic and molecular studies on animal models and patients with Beckwith-Wiedemann syndrome have shown that alterations in CDKN1C (the p57Kip2 encoding gene) have functional relevance in the pathogenesis of this disease. Recently, a number of investigations have identified and characterized heretofore unexpected roles for p57Kip2. The protein appears to be critically involved in initial steps of cell and tissue differentiation, and particularly in neuronal development and erythropoiesis. Intriguingly, p27Kip1, the Cip/Kip member that is most homologous to p57Kip2, is primarily involved in the process of cell cycle exit. p57Kip2 also plays a critical role in controlling cytoskeletal organization and cell migration through its interaction with LIMK-1. Furthermore, p57Kip2 appears to modulate genome expression. Finally, accumulating evidence indicates that p57Kip2 protein is frequently downregulated in different types of human epithelial and nonepithelial cancers as a consequence of genetic and epigenetic events. In summary, the emerging picture is that several aspects of p57Kip2's functions are only poorly clarified. This review represents an appraisal of the data available on the p57Kip2 gene and protein structure, and its role in human physiology and pathology. We particularly focus our attention on p57Kip2 changes in cancers and pharmacological approaches for modulating p57Kip2 levels. Mol Cancer Res; 9(10); 1269–84. ©2011 AACR.

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“…The mouse insulin-like growth factor II (Igf2) and H19 genes lie within a large imprinted region on distal chromo some 7 (5). The two genes are separated by only 70 kb and imprinted in opposite directions: only the paternal allele of Ig/2 is expressed and only the maternal allele of H19 is ex pressed (6, 7) ( Fig.…”

mentioning

confidence: 99%

Mechanisms of Igf2/H19 Imprinting: DNA Methylation, Chromatin and Long-Distance Gene Regulation

Sasaki

Ishihara

Kato

2000

Journal of Biochemistry

Self Cite

106

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The mouse Igf2 and H19 genes lie 70-kb apart on chromosome 7 and are reciprocally imprinted. Two regulatory regions are important for their parental allele-specific expression: a differentially methylated region (DMR) upstream of H19 and a set of tissue-specific enhancers downstream of H19. The enhancers specifically activate Igf2 on the paternal chromosome and H19 on the maternal chromosome. The interactions between the enhancers and the genes are regulated by the DMR, which works as a selector by exerting dual functions: a methylated DMR on the paternal chromosome inactivates adjacent H19 and an unmethylated DMR on the maternal chromosome insulates Igf2 from the enhancers. These processes appear to involve methyl-CpG-binding proteins, histone deacetylases and the formation of chromatin insulator complexes. The Igf2/H19 region provides a unique model in which to study the roles of DNA methylation and chromatin structure in the regulation of chromosome domains.

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Sequence-ready 1-Mb YAC, BAC and Cosmid Contigs Covering the Distal Imprinted Region of Mouse Chromosome 7

Cited by 10 publications

References 13 publications

Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH

Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH

p57Kip2 and Cancer: Time for a Critical Appraisal

Mechanisms of Igf2/H19 Imprinting: DNA Methylation, Chromatin and Long-Distance Gene Regulation

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