Sequence Polymorphisms in
            <i>Vibrio cholerae</i>
            HapR Affect Biofilm Formation under Aerobic and Anaerobic Conditions

Caigoy, Jant Cres; Mukhopadhyay, Asish K.; Shinoda, Sumió; Shimamoto, Tadashi

doi:10.1128/aem.01044-22

Cited by 5 publications

(1 citation statement)

References 72 publications

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“…A lower biofilm production in the anaerobic environment was observed, which is consistent with the reduced growth under anaerobic in comparison to aerobic conditions. A previous study also drawn similar conclusions, stating that the hypoxic environment limits biofilm formation in V. cholerae compared to an oxygen-present environment (42). Besides, we observed that biofilm production is inhibited from 25 µg/ml of PmB in all strains under aerobic conditions.…”

Section: Discussionsupporting

confidence: 90%

Elevated concentrations of polymyxin B elicit a biofilm-specific resistance mechanism inVibrio cholerae

Pauzé-Foixet,

Duperthuy

2023

Preprint

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Vibrio cholerae can form biofilms both in the aquatic environment and in the human intestine, facilitating the release of hyper-infectious aggregates. Due to the increasing antibiotic resistance that impedes the treatment of infections, alternatives need to be found. One of these alternatives is antimicrobial peptides, including polymyxin B (PmB), which is already used to treat infections caused by antibiotic-resistant bacteria. In this study, we first investigated the resistance of V. cholerae O1 El Tor strain A1552 to various antimicrobials under aerobic and anaerobic conditions. Increased resistance to PmB is observed in anaerobiosis, with a 3-fold increase in the dose required for 50% growth inhibition. We then studied the impact of the PmB on the formation and degradation of V. cholerae biofilms to PmB. Our results show that PmB affects more efficiently biofilm formation under anaerobic conditions. On the other hand, performed biofilms are susceptible to degradation by PmB at concentrations close to the minimum inhibitory concentration (MIC), resulting in approximately 50% reduction of the biomass. At higher concentrations, we observed less degradation and opacification of the biofilm structures within 20 minutes post-treatment, suggesting densification of the structure. This densification does not seem to result from the overexpression of matrix genes but rather from the release of DNA through cellular lysis, forming a protective shield that limits the penetration of the PmB into the biofilm.

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Section: Discussionsupporting

confidence: 90%

Elevated concentrations of polymyxin B elicit a biofilm-specific resistance mechanism inVibrio cholerae

Pauzé-Foixet,

Duperthuy

2023

Preprint

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Genotype-phenotype evaluation of the heterogeneity in biofilm formation by diverse Bacillus licheniformis strains isolated from dairy products

Fan,

Liu,

Dai

et al. 2024

International Journal of Food Microbiology

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Development of a simple allele-specific PCR for the detection of pathogenic Vibrio cholerae O1 and O139 in seafood

Caigoy,

Shimamoto,

Shimamoto

2024

Journal of Agriculture and Food Research

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Sequence Polymorphisms in Vibrio cholerae HapR Affect Biofilm Formation under Aerobic and Anaerobic Conditions

Abstract: Our study revealed an ancestral hapR sequence from a phylogenetic reconstruction that displayed the evolutionary lineage of the nonpandemic to the pandemic strains. Here, we established hapR1 and hapR2 as major hapR haplogroups.

Cited by 5 publications

References 72 publications

Elevated concentrations of polymyxin B elicit a biofilm-specific resistance mechanism inVibrio cholerae

Elevated concentrations of polymyxin B elicit a biofilm-specific resistance mechanism inVibrio cholerae

Genotype-phenotype evaluation of the heterogeneity in biofilm formation by diverse Bacillus licheniformis strains isolated from dairy products

Development of a simple allele-specific PCR for the detection of pathogenic Vibrio cholerae O1 and O139 in seafood

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