Sequence polymorphisms at the growth hormone GH1/GH2-N and GH2-Z gene copies and their relationship with dairy traits in domestic sheep (Ovis aries)

Vacca, Giuseppe Massimo; Dettori, Maria Luisa; Balia, Filippo; Luridiana, Sebastiano; Mura, Maria Consuelo; Carcangiu, Vincenzo; Pazzola, Michele

doi:10.1007/s11033-013-2629-9

Cited by 13 publications

(18 citation statements)

References 21 publications

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“…The data from ewes that presented evidence of clinical disease or mastitis were excluded from milk sampling. The milk composition (low casein number, lactose and urea content, high pH, and particularly, SCS), when compared with the Sarda sheep in similar farming systems (Mura et al, 2012;Vacca et al, 2013), still suggested that the ewes that produced the NC samples were affected by subclinical mastitis, which is known to negatively affect milk coagulation and cheese yield in ovine species (Merin et al, 2004).…”

Section: Noncoagulating Milk Samplesmentioning

confidence: 99%

Modeling of coagulation, curd firming, and syneresis of milk from Sarda ewes

Vacca

Pazzola

Dettori

et al. 2015

Journal of Dairy Science

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This study investigated the modeling of curd-firming (CF) over time (CF(t)) of sheep milk. Milk samples from 1,121 Sarda ewes from 23 flocks were analyzed for coagulation properties. Lactodynamographic analyses were conducted for up to 60 min, and 240 CF individual observations from each sample were recorded. Individual sample CFt equation parameters (RCT(eq), rennet coagulation time; CF(P), asymptotic potential value of curd firmness; k(CF), curd-firming instant rate constant; and k(SR), curd syneresis instant rate constant) were estimated, and the derived traits (CF(max), the point at which CF(t) attained its maximum level, and tmax, the time at which CF(max) was attained) were calculated. The incidence of noncoagulating milk samples was 0.4%. The iterative estimation procedure applied to the individual coagulation data showed a small number of not-converged samples (4.4%), which had late coagulation and an almost linear pattern of the ascending part of the CF(t) curve that caused a high value of CF(P), a low value of k(CF), and a high value of k(SR). Converged samples were classified on the basis of their CF(t) curves into no-k(SR) (18.0%), low-k(SR) (72.6%), and high-k(SR) (4.5%). A CF(t) that was growing continuously because of the lack of the syneresis process characterized the no-k(SR) samples. The high-k(SR) samples had a much larger CFP, a smaller k(CF), and an anticipation of tmax, whereas the low-k(SR) samples had a fast k(CF) and a slower k(SR). The part of the average CF(t) curves that showed an increase was similar among the 3 different syneretic groups, whereas the part that decreased was different because of the expulsion of whey from the curd. The traditional milk coagulation properties recorded within 30 min were not able to detect any appreciable differences among the 4 groups of coagulating samples, which could lead to a large underestimation of the maximum CF of all samples (if predicted by a30), with the exception of the no-k(SR) samples. Large individual variability was found and was likely caused by the effects of the dairy system, such as flock size (on CF(max), t(max), and % ewes with no-k(SR) milk), flock within flock size (representing 11 to 43% of total variance for % ewes with no-k(SR) milk and CF(max), respectively), days in milk (on all model parameters and CF(max)), parity (on RCT(eq), k(SR), and CF(max)), daily milk yield (on RCT(eq) and CF(max)), and position of the individual pendulum that significantly affected model parameters and derived traits. In conclusion, the results showed that the modeling of coagulation, curd-firming, and syneresis is a suitable tool to achieve a deeper interpretation of the coagulation and curd-firming processes of sheep milk and also to study curd syneresis.

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Section: Noncoagulating Milk Samplesmentioning

confidence: 99%

Modeling of coagulation, curd firming, and syneresis of milk from Sarda ewes

Vacca

Pazzola

Dettori

et al. 2015

Journal of Dairy Science

Self Cite

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“…However, this may also have some negative effects. Some literature indicates that the variants led to increased milk components, but reduced the milk yield [46][47][48]. Regrettably, in this study, because of the lack of data on the milk yield of dairy goats, the effect of this indel on this aspect cannot be studied.…”

Section: Discussionmentioning

confidence: 85%

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Zhang

Wang

Liu

et al. 2019

Animals

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Simple Summary: In the field of animal breeding, selection using molecular genetic markers, such as insertions/deletions (indels), is a novel method to reduce the generation interval and more accurately improve performance. The casein alpha s1 (CSN1S1) gene is one of the major genes regulating the milk performance of mammals, and it is also relevant to body development. Previous studies have shown that an 11-bp indel in this gene was strongly associated with goat litter size, however, its effects on milk performance and body measurement traits have not been studied. In this study, this indel was genotyped in three Chinese goat breeds, and further analysis results revealed that this indel polymorphism was significantly associated with milk performance in dairy goats, as well as affecting the body measurement traits in all three breeds. These findings hint that the 11-bp indel within CSN1S1 gene could be utilized for effectively selecting goats with excellent performances in terms of production and breeding.Abstract: The casein alpha s1 (CSN1S1) gene encodes α-s1 casein, one of the proteins constituting milk, which affects milk performance, as well as improving the absorption of calcium and bone development in mammals. A previous study found that an 11-bp insertion/deletion (indel) of this gene strongly affected litter size in goats. However, to our knowledge, the relationships between this polymorphism and the milk performance and body measurement traits of goats have not been reported. In this paper, the previously identified indel has been recognized in three Chinese goat breeds, namely the Guanzhong dairy goat (GZDG; n = 235), Shaanbei white cashmere goat (SBWC; n = 1092), and Hainan black goat (HNBG; n = 278), and the following three genotypes have been studied for all of the breeds: insertion/insertion (II), deletion/deletion (DD), and insertion/deletion (ID). The allele frequencies analyzed signified that the frequencies of the "D" allele were higher (47.8%-65.5%), similar to the previous report, which indicates that this polymorphism is genetically stable in different goat breeds. Further analysis showed that this indel was markedly associated with milk fat content, total solids content, solids-not-fat content, freezing point depression, and acidity in GZDG (p < 0.05), and also affected different body measurement traits in all three breeds (p < 0.05). The goats with II genotypes had superior milk performance, compared with the others; however, goats with DD genotypes had better body measurement sizes. Hence, it may be necessary to select goats with an II or DD genotype, based on the desired traits, while breeding. Our study Animals 2019, 9, 1114 2 of 11 provides information on the potential impact of the 11-bp indel polymorphism of the CSN1S1 gene for improving the milk performance and body measurement traits in goats.

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“…The GH of sheep was sequenced by different studies, it was 2,055 and 2,219 bp long for GH1/ GH2-N and GH2-Z in Sarda sheep (Vacca et al, 2013), 2162 bp (Orian et al, 1988) and 1629 bp for Lohi sheep (Gul et al, 2009).…”

Section: Correlations and Regression Analysis Resultsmentioning

confidence: 99%

“…DQ46164. Two were located at the 5\ UTR, two at the 3\ UTR; one was located in exon-5 (G556A) and resulted in a putative amino acid substitution G186S (Vacca et al, 2013). Seven SNPs were detected in a Portuguese sheep breed (Bastos et al, 2001).…”

Section: Correlations and Regression Analysis Resultsmentioning

confidence: 99%

Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep

Abdelmoneim

Brooks

Afifi

et al. 2016

IJAR

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This study aimed to evaluate the relationship between growth hormone (GH) gene polymorphism and estimated body weight in Harri sheep. One hundred Harri sheep lambs were used to determine the birth weight (BW) and weight at 120 days of age. The daily live-weight gain (DLWG) 0-120 days (g) was calculated. The complete CDS of the Harri sheep GH gene is 2117pb in length (GenBank acc. no. KU255857). Three novel SNPs were detected by comparing with GenBanke acc. no. X12546_1. The G871A SNP in intron II, G1383A in exon IV that resulted in conversion of the amino acid arginine number 121 to lysine (R121K) and the A1509G in intron IV. Each SNP was found on both alleles the mutant homozygote was more common (48, 56 and 50%) than the heterozygote (30, 18 and 20%) for G871A, G1383A and A1509G respectively. A positive significant (P<0.05) correlation between growth traits (BW, 120 day body weight and DLWG) and SNP and a highly significant correlation with the genotype were detected. The regression analysis indicated the positive effect of genotype and SNPs on the growth traits. Individuals carrying homozygote mutant alleles had the heaviest body weight and the highest DLWG. Consequently, these SNP may be useful indicators in the selection of lambs for higher growth rate and meat production.

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Sequence polymorphisms at the growth hormone GH1/GH2-N and GH2-Z gene copies and their relationship with dairy traits in domestic sheep (Ovis aries)

Cited by 13 publications

References 21 publications

Modeling of coagulation, curd firming, and syneresis of milk from Sarda ewes

Modeling of coagulation, curd firming, and syneresis of milk from Sarda ewes

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep

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