Sequence of the short unique region, short repeats, and part of the long repeats of human cytomegalovirus

Weston, Kathleen; Barrell, Bart

doi:10.1016/0022-2836(86)90359-1

Cited by 115 publications

(98 citation statements)

References 29 publications

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“…Shope fibroma virus, a leporipoxvirus, contains three similar genes within each ITR (Upton et al, 1987), and fowlpox virus, an avipoxvirus, contains a family of genes near the left end of the genome whose products are related to hepatic lectins (Tomley et aL, 1988). Other gene families have been found in African swine fever virus (Blasco et aL, 1988) and in human cytomegalovirus (Weston & Barrell, 1986). These large DNA viruses appear to have increased their genome complexity by gene duplication events and subsequent divergence of duplicated members.…”

Section: Discussionmentioning

confidence: 99%

Two Early Vaccinia Virus Genes Encode Polypeptides Related to Protein Kinases

Howard

Smith

1989

Journal of General Virology

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SUMMARYVaccinia virus particles contain a protein kinase with an Mr of 62K calculated from sedimentation rate. We have sequenced the SalI G restriction fragment of the v accinia virus genome near to the right inverted terminal repeat and have identified two genes which share 36~ amino acid identity with each other and are related to the family of protein kinase genes. One gene, designated B1 R, encodes a 34.2K protein which shares 27~ identity with a protein kinase encoded by the herpes simplex virus type 1 US3 gene and contains conserved motifs characteristic of protein kinases of serine/ threonine specificity. The second gene, BI2R, encodes a protein of 33-3K which is poorly related to known protein kinases and lacks specific amino acids at several highly conserved key positions. The deduced partial amino acid sequence of a gene in the corresponding region of the cowpox virus genome is identical to B 12R except for one conservative amino acid substitution. Both of the vaccinia virus genes are transcribed towards the right-hand end of the genome early during infection. It is possible that the product of either or both of these genes associates to form a homo-or heterodimer that represents the 62K virion-associated protein kinase.

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Section: Discussionmentioning

confidence: 99%

Two Early Vaccinia Virus Genes Encode Polypeptides Related to Protein Kinases

Howard

Smith

1989

Journal of General Virology

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“…The HHV-6 ORFs SHLI, SHL2, SSL2 and SFLI are related to one or more members of the HCMV US22 gene family, which consists of ORFs US22, US23, US24, US26, IRS1/TRS1, UL23, UL24, UL28, UL29, UL36 and UL43 (Weston & Barrell, 1986;Kouzarides et al, 1988;Chee et al, 1990). A summary of the overall amino acid sequence similarity between these proteins is shown by the FASTA score matrix (Table 3).…”

Section: Hhv-6 0rfs With Homology To the Hcmv Us22 Gene Familymentioning

confidence: 99%

Identification of homologues to the human cytomegalovirus US22 gene family in human herpesvirus 6

Efstathiou

Lawrence²,

Brown³

et al. 1992

Journal of General Virology

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“…Within the short unique region, Weston & Barrell (1986) have identified at least 38 regions that may code for proteins. The number and distribution of polyadenylation signals in relation to the ORFs indicate that many of the mRNAs generated will have 3' coterminal ends, a phenomenon common to all herpesviruses (McGeoch et al, 1985).…”

Section: Proteins From the Us Regionmentioning

confidence: 99%

“…In summary, this indicates that HCMV strains are relatively stable in nucleotide (nt) sequence and are well conserved, most heterogeneity being represented by a limited restriction enzyme cleavage polymorphism due to sequence divergence. Weston & Barrell (1986) have determined a 46 kb DNA sequence containing the short unique region (Us), the short repeat (Rs) and part of the long repeat (RL). This is the largest piece of continuous HCMV DNA sequence reported so far.…”

mentioning

confidence: 99%