2002
DOI: 10.1016/s0378-1119(02)00876-4
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Sequence of the Escherichia coli O26 O antigen gene cluster and identification of O26 specific genes

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Cited by 59 publications
(66 citation statements)
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“…Furthermore, the sequences obtained after a mutagenesis assay showed a high degree of identity with genes involved in LPS biosynthesis pathways. The fnl-1 gene is one of the three genes that seem to be responsible for the synthesis of N-acetyl-L-fucosamine, one of the sugars composing the O26 O antigen (7). The wbjB gene from Pasteurella multocida and Pseudomonas aeruginosa and the rfb gene cluster of L. interrogans are also involved in the O-antigen biosynthesis pathway (6,16).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Furthermore, the sequences obtained after a mutagenesis assay showed a high degree of identity with genes involved in LPS biosynthesis pathways. The fnl-1 gene is one of the three genes that seem to be responsible for the synthesis of N-acetyl-L-fucosamine, one of the sugars composing the O26 O antigen (7). The wbjB gene from Pasteurella multocida and Pseudomonas aeruginosa and the rfb gene cluster of L. interrogans are also involved in the O-antigen biosynthesis pathway (6,16).…”
Section: Discussionmentioning
confidence: 99%
“…The wbjB gene from Pasteurella multocida and Pseudomonas aeruginosa and the rfb gene cluster of L. interrogans are also involved in the O-antigen biosynthesis pathway (6,16). The wbuA and wbuB genes belong to the O-antigen gene cluster from the O26:K60: H311b E. coli strain and code for a putative rhamnosyl transferase and a putative L-fucosamine transferase, respectively (7).…”
Section: Discussionmentioning
confidence: 99%
“…The sequences of sugar transferase genes and O-unitprocessing genes are normally specific to a particular O antigen. Specific PCR methods based on O-antigen-specific genes have been proposed for molecular typing of many E. coli and Shigella O serogroups (21,25,26,28,54,(57)(58)(59).…”
mentioning
confidence: 99%
“…ORFs 11, 12, and 13 showed 81, 57, and 70% identity to FnlA (WbjB), FnlB (WbjC), and FnlC (WbjD), respectively, of the Pseudomonas aeruginosa O11 O-antigen gene cluster (GenBank entries AAF72954, AAF72955, and AAF72956) (19); 79, 43, and 50% identity to FnlA (Cap5E), FnlB (Cap5F), and FnlC (Cap5G), respectively, of the Staphylococcus aureus type 5 capsule gene cluster (GenBank entries AAC46088, AAC46089, and AAC46090); and 88, 71, and 89% identity to FnlA, FnlB, and FnlC, respectively, of the E. coli O26 O-antigen gene cluster (21). FnlA, FnlB, and FnlC are enzymes of the UDP-L-FucNAc biosyn- (GenBank accession number AAG06538) and shared the motif SGGLDSS with homologues of WbpG.…”
mentioning
confidence: 99%
“…Our group, among others, has been working on DNA-based O-serotyping methods in Shigella and E. coli with an objective of developing a DNA-based typing scheme. Recently, a number of reports have shown that using PCR assays based on O-antigen specific genes can distinguish particular Shigella and E. coli O-serogroups (Wang et al 1998;Wang et al 1998;Wang et al 2001a;D'Souza et al 2002;Perelle et al 2002;DebRoy et al 2003;Fratamico et al 2003;Feng et al 2004aFeng et al , 2004bFeng et al , 2004cTao et al 2004). As an alternative to the conventional serotyping assay, which is time consuming, subject to serological cross-reactions, and inaccurate because of the presence of smooth and rough forms of LPS produced by bacteria, the PCR method is accurate, sensitive, and rapid.…”
Section: Introductionmentioning
confidence: 99%