Sequence of the 50-kb Conjugative Multiresistance Plasmid pRE25 from Enterococcus faecalis RE25

Schwarz, Franziska; Perreten, Vincent; Teuber, Michael

doi:10.1006/plas.2001.1544

Cited by 155 publications

(125 citation statements)

References 42 publications

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“…A 30.5-kb segment of pRE25 was found to be highly similar to pIP501. The chloramphenicol acetyltransfer- ase gene, the 23S RNA methylase gene, and part of the putative conjugative unit of pRE25 (oriT and orf24 to orf29) showed 100% identity to the pIP501 49-bp oriT region and the first six genes of the pIP501 transfer operon (186). The 30.5-kb segment of pRE25 is flanked by two IS1216V elements and is also highly similar to the two other plasmids, pSM19035 (34) and pAM␤1 (28).…”

Section: Conjugative Transfer Of Broad-host-range Plasmidsmentioning

confidence: 95%

“…The available information suggests that the major differences between conjugation in gramnegative and gram-positive bacteria lie in the mechanisms that have evolved to establish cell-cell contact in order to initiate conjugal transfer. Determination of the nucleotide sequence of the tra regions or whole genomes of several large conjugative plasmids from gram-positive bacteria (15,59,143,186) has revealed homologies to proteins belonging to the TraG/TrwB/ VirD4 family of coupling proteins (for recent reviews and meeting reports on type IV secretion systems, see references 11, 30, 33, 36, 37, 51, 119, and 193), to the conjugative transfer ATPase VirB4 and its homologues (51,52) involved in substrate translocation processes during T-DNA and plasmid transfer in gram-negative bacteria, and to the VirB1 family of lytic transglycosylases. These interesting homologies to components of type IV secretion systems are discussed below.…”

Section: Conjugative Transfer In Unicellular Gram-positive Bacteriamentioning

confidence: 99%

“…The complete nucleotide sequences of the staphylococcal plasmid pSK41 (15,71), the lactococcal plasmid pMRC01 (59), and the enterococcal plasmid pRE25 (186) have been determined. A 30.5-kb segment of pRE25 was found to be highly similar to pIP501.…”

Section: Conjugative Transfer Of Broad-host-range Plasmidsmentioning

confidence: 99%

“…The restriction map of pIP501 also exhibits a considerable degree of identity to the corresponding 30.5-kb fragment of pRE25. These data led to the hypothesis that pRE25 is pIP501 enlarged by an IS1216V-induced insertion (186). The entire transfer region of the staphylococcal self-transmissible plasmid pGO1 has also been sequenced (143), and very recently we determined the 3Ј part of the pIP501 tra region encompassing orf7-15 (accession number AJ505823).…”

Section: Conjugative Transfer Of Broad-host-range Plasmidsmentioning

confidence: 99%

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Conjugative Plasmid Transfer in Gram-Positive Bacteria

Grohmann

Muth

Espinosa

2003

Microbiol Mol Biol Rev

495

447

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Conjugative transfer of bacterial plasmids is the most efficient way of horizontal gene spread, and it is therefore considered one of the major reasons for the increase in the number of bacteria exhibiting multiple-antibiotic resistance. Thus, conjugation and spread of antibiotic resistance represents a severe problem in antibiotic treatment, especially of immunosuppressed patients and in intensive care units. While conjugation in gram-negative bacteria has been studied in great detail over the last decades, the transfer mechanisms of antibiotic resistance plasmids in gram-positive bacteria remained obscure. In the last few years, the entire nucleotide sequences of several large conjugative plasmids from gram-positive bacteria have been determined. Sequence analyses and data bank comparisons of their putative transfer (tra) regions have revealed significant similarities to tra regions of plasmids from gram-negative bacteria with regard to the respective DNA relaxases and their targets, the origins of transfer (oriT), and putative nucleoside triphosphatases NTP-ases with homologies to type IV secretion systems. In contrast, a single gene encoding a septal DNA translocator protein is involved in plasmid transfer between micelle-forming streptomycetes. Based on these clues, we propose the existence of two fundamentally different plasmid-mediated conjugative mechanisms in gram-positive microorganisms, namely, the mechanism taking place in unicellular gram-positive bacteria, which is functionally similar to that in gram-negative bacteria, and a second type that occurs in multicellular gram-positive bacteria, which seems to be characterized by double-stranded DNA transfer

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Section: Conjugative Transfer Of Broad-host-range Plasmidsmentioning

confidence: 95%

Section: Conjugative Transfer In Unicellular Gram-positive Bacteriamentioning

confidence: 99%

Section: Conjugative Transfer Of Broad-host-range Plasmidsmentioning

confidence: 99%

Section: Conjugative Transfer Of Broad-host-range Plasmidsmentioning

confidence: 99%

See 2 more Smart Citations

Conjugative Plasmid Transfer in Gram-Positive Bacteria

Grohmann

Muth

Espinosa

2003

Microbiol Mol Biol Rev

495

447

View full text Add to dashboard Cite

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“…The ⑀/ TA family is commonly found on plasmids (17)(18)(19)(20) and chromosomes of many human pathogens (21), including both Gram-positive and Gram-negative bacteria (22,23). Information concerning this TA family has been gathered from many years of studies on the ⑀/ system from the pSM19035 plasmid, which was originally isolated from the clinical strain of Streptococcus pyogenes (24) and was expanded by studies on the homologous PezAT system in Streptococcus pneumoniae (25,26).…”

mentioning

confidence: 99%