Sequence of oligodendrocyte development in the human fetal telencephalon

Jakovčevski, Igor; Zečević, Nada

doi:10.1002/glia.20134

Cited by 113 publications

(118 citation statements)

References 73 publications

(101 reference statements)

Supporting

Mentioning

108

Contrasting

Order By: Relevance

“…Conversely, numerous MBP reactive oligodendrocytes are present in five days old slices (Fig. 5E), but not in the brain sections from the same gestational age and regions (Jakovcevski and Zecevic, 2005a). Most notably, MBP+ processes on slices appear to follow the axons, suggesting myelination (Jakovcevski and Zecevic, 2005a).…”

Section: Psa-ncam Expression In Organotypic Slice Culturesmentioning

confidence: 91%

“…2C-E; 4C, D). During midgestation, the first MBP+ pre-myelinating oligodendrocytes appear in the telencephalic white matter (Jakovcevski and Zecevic, 2005a). In all forebrain regions studied (corpus callosum, intermediate zone, and internal capsule), MBP+ myelinating oligodendrocytes are present only in areas with low level of PSA-NCAM expression on axons.…”

Section: Expression Pattern Of Psa-ncam On Axonal Tracts At Midgestationmentioning

confidence: 91%

“…Immunohistochemistry was performed as described before (Jakovcevski and Zecevic, 2005a). Various primary antibodies and corresponding fluorescein-and rhodamine-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA) were used (see above).…”

Section: Immunofluorescence and Image Analysismentioning

confidence: 99%

“…In order to test whether PSA-NCAM expression changes during progression of myelination, we analyzed slice cultures from human fetal subventricular and intermediate zones at midgestation (Jakovcevski and Zecevic, 2005a). Initially in slice cultures PSA-NCAM is abundant in the intermediate zone (Fig.…”

Section: Psa-ncam Expression In Organotypic Slice Culturesmentioning

confidence: 99%

“…The level of myelination was estimated by the expression of myelin basic protein (MBP), a marker of terminally differentiated oligodendrocytes (Pfeiffer et al, 1993;Jakovcevski and Zecevic, 2005a). In organotypic slice culture of human fetal forebrain, the increased MBP expression was demonstrated after 3-5 days in vitro (Jakovcevski and Zecevic, 2005a).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Down-regulation of the axonal polysialic acid–neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain

Jakovčevski

Zečević

2007

Neuroscience

View full text Add to dashboard Cite

The polysialic acid (PSA) modification of neural cell adhesion molecule, which reduces NCAMmediated cell adhesion, is involved in several developmental processes, such as cell migration, axonal growth, pathfinding, and synaptic plasticity. It has been suggested that PSA-NCAM expression may inhibit myelination. To clarify the relationship between myelination and the expression of PSA-NCAM we systematically investigated its expression in the human forebrain from embryonic stage to midgestation (19-24 gestation weeks, gw). Immunofluorescence on cryosections showed that PSA-NCAM is expressed at the earliest stage studied (5.5 gw) in the primordial plexiform layer of the telencephalon, which mainly consists of neuronal processes. At midgestation, cortical axonal tracts in the emerging white matter were PSA-NCAM+, but they were not yet myelinated, based on the lack of myelin basic protein (MBP) immunoreaction. To follow the progression of myelination we developed organotypic slice cultures that included the subventricular and intermediate zones of the fetal forebrain. In freshly prepared slices, similar to cryosections, axonal tracts were PSA-NCAM + but did not express MBP. After 5 days in culture there was a dramatic increase in MBP expression around the axons of the intermediate zone, which suggested the onset of myelination. Simultaneously with MBP up-regulation PSA-NCAM expression in axons was completely lost, as demonstrated both with immunofluorescence and Western blot analysis. These results support the idea that in the human fetal forebrain axonal PSA-NCAM expression is inversely related to primary myelination.

show abstract

Section: Psa-ncam Expression In Organotypic Slice Culturesmentioning

confidence: 91%

Section: Expression Pattern Of Psa-ncam On Axonal Tracts At Midgestationmentioning

confidence: 91%

Section: Immunofluorescence and Image Analysismentioning

confidence: 99%

Section: Psa-ncam Expression In Organotypic Slice Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Down-regulation of the axonal polysialic acid–neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain

Jakovčevski

Zečević

2007

Neuroscience

View full text Add to dashboard Cite

show abstract

Heterogeneity of oligodendrocyte progenitor cells in adult human brain

Leong

Rao

Bin

et al. 2014

Ann Clin Transl Neurol

View full text Add to dashboard Cite

ObjectiveRemyelination in multiple sclerosis has been attributed to the presence of oligodendrocyte progenitor cells (OPCs) in brain parenchyma. However, the precise identity of these progenitors is poorly defined. Here, we characterized populations of OPCs in the adult human brain and examined their myelination capacity and profile of miRNAs. Comparisons were made with fetal OPCs and mature oligodendrocytes.MethodsWe isolated human adult and fetal (early-to-mid second trimester) OPCs from surgically resected brain tissues using O4-, A2B5-, and MOG-directed fluorescence activated cell sorting and transplanted them into dysmyelinated shiverer slices to examine their myelination capacity. We used qRT-PCR to analyze expression of selective miRNAs implicated in OPC biology.ResultsThree subsets of putative OPCs were identified in adult brains: (1) A2B5(+), (2) O4low, and (3) A2B5(+)O4highMOG(+) progenitors. In comparison, fetal brains contained (1) A2B5(+), (2) O4(+), and (3) A2B5(+)O4(+) progenitors, but no MOG(+) cells. We demonstrate that like fetal OPCs, adult OPCs have the capacity to ensheathe cerebellar axons. However, adult OPCs exhibit low to undetectable expression of miRNAs that were highly expressed in O4-expressing fetal OPCs. Adult OPCs also express different miRNAs compared to mature oligodendrocytes.InterpretationWe conclude that phenotypically distinct subsets of OPCs are present in adult human brain and these OPCs show differential miRNA expression compared to fetal OPCs and mature oligodendrocytes. These suggest that remyelination in adult brain may involve multiple populations of progenitors within the brain and that OPC differentiation in adulthood may be differentially regulated compared to development.

show abstract

Contributions of cortical subventricular zone to the development of the human cerebral cortex

2005

Self Cite

View full text Add to dashboard Cite

The cortical subventricular zone (SVZ), a proliferative compartment in the forebrain, has a uniquely important role during the second half of intrauterine development in human. This is best observed in numerous neonatal pathologies that result from prenatal SVZ damage. These conditions highlight a need to better understand the contribution of the SVZ to the development of the human cerebral cortex. With this goal in mind, we analyze histological organization, cell proliferation, and molecular diversity in the human fetal SVZ, from 7 to 27 gestational weeks (gw), using light and electron microscopy, immunohistochemistry, and in vitro methods. Complex histological organization distinguishes human cortical SVZ from that of other mammals. In vitro quantification showed that approximately 50% of cells in the VZ/SVZ region are neurons, 30% are astroglia, 15% are nestin + cells, with other cell types representing smaller fractions. Immunolabeling with BrdU, showed that a considerable number of cells (approximately 10%) are generated in the human cortical SVZ during midgestation (18-24gw), in in vitro conditions. Immunofluorescence with cell type specific markers and BrdU revealed that all major cell types, neural precursors (nestin + ), astroglia including radial glia (GFAP + , vimentin + ), and oligodendrocyte progenitors (PDGFR-α + ), were proliferating. An increase in the ratio of the size of the SVZ to VZ, protracted period of cell proliferation, as well as cellular and histological complexity of the human fetal SVZ, are directly related to the evolutionary expansion of the human cerebral cortex.

show abstract

Sequence of oligodendrocyte development in the human fetal telencephalon

Cited by 113 publications

References 73 publications

Down-regulation of the axonal polysialic acid–neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain

Down-regulation of the axonal polysialic acid–neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain

Heterogeneity of oligodendrocyte progenitor cells in adult human brain

Contributions of cortical subventricular zone to the development of the human cerebral cortex

Contact Info

Product

Resources

About