Sequence of histidyl tRNA, present as a chloroplast insert in mtDNA ofZea mays

Abstract

Unfractionated tRNA, isolated from maize mitochondria, has been specifically labeled at the -CCA end and used to recover a tRNA gene-bearing fragment from a clone bank of maize mitochondrial DNA. This gene has been mapped, sequenced and found to carry the anticodon for histidine. The sequence of the gene and that of bases in its near vicinity are identical to maize chloroplast tRNA(His), although sequences more distant on the fragment are not homologous with cpDNA. The junction of the cpDNA insert has been sequenced.

Identification and Structural Characterization of Nucleus-encoded Transfer RNAs Imported into Wheat Mitochondria

“…This is in contrast not only to the situation in several dicots (53, 54) but also several other graminaceous plants (55)(56)(57), where the mt-tRNA…”

Sequence and transcriptional analysis of a chloroplast insert in the mitochondrial genome ofZea mays

“…Maize mtDNA Offprint requests to: W. W. Hauswirth was found to contain coding sequences for the chloroplast 16S ribosomal RNA and for the adjacent isoleucine rRNA and valine rRNA genes (Stern and Lonsdale 1982). More recently, other transposed chloroplast sequences, including those for the large subunit polypeptide of ribulose 1,5 bisphosphate carboxylase (Lonsdale et al 1983), tRNA gene sequences for histidine tRNA (Iams et al 1985) and part of the 23S rRNA gene and downstream sequences (Lonsdale 1985) were identified. To date a complete nucleotide sequence has been reported only for the histidine tRNA gene insert (Iams et al 1985).…”

“…More recently, other transposed chloroplast sequences, including those for the large subunit polypeptide of ribulose 1,5 bisphosphate carboxylase (Lonsdale et al 1983), tRNA gene sequences for histidine tRNA (Iams et al 1985) and part of the 23S rRNA gene and downstream sequences (Lonsdale 1985) were identified. To date a complete nucleotide sequence has been reported only for the histidine tRNA gene insert (Iams et al 1985). A partial sequence has also been preliminarily reported for the 23S rRNA gene insert (Braun and Levings 1986).…”

Comparison of regions coding for tRNAHis genes of mitochondrial and chloroplast DNA in sunflower: a proposal concerning the classification of ?CP-like? tRNA genes

“…The comparison of its flanking regions with those of the mitochondrial counterpart reveals that the mitochondrial gene does not belong to an evident cpDNA insertion as in the case of maize [7].…”

“…The sequencing of this fragment (Fig. 1) revealed the presence of a tRNA His gene identical to the maize chloroplast and mitochondrial genes [7]. Only two T~C transitions differentiate it from its sunflower and Oenothera mitochondrial counterparts [3,2],…”