Sequence of a cDNA encoding turtle riboflavin-binding protein: a comparison with avian riboflavin-binding protein

Down-regulation of free riboflavin content induces hydrogen peroxide and a pathogen defense in Arabidopsis

“…So far, the protein has been identified in other oviparous species in addition to chicken (Maehashi et al 2009), amphibian (Storey et al 1999), fish (Wang et al 2003), and mammals (Natraj et al 1988). In ovipara, the RfBP gene is expressed in the liver and the oviduct in an estrogen-dependent manner, and in oocytes subsequent to fecundation (Hamajima and Ono 1995;Zheng et al 1988;Maehashi et al 2009). The estrogen-dependent and fecundation-induced expression patterns are also found in other animals (Storey et al 1999;Natraj et al 1988;Wang et al 2003).…”

“…Soft-shelled turtle (Trionyx sinensis japonicus) TsRfBP shows a high identity to the chicken (Gallus domestiaus) GdRfBP in amino acid sequence, especially in the ligandbinding domain and the phosphorylated domain (Hamajima and Ono 1995;Sabharanjak and Mayor 2004;Bedhomme et al 2005;Roje 2007). The ligand-binding domain is implicated in the interaction between RfBP and the plasma membrane receptor (Latha et al 2008).…”

“…The ligand-binding domain is implicated in the interaction between RfBP and the plasma membrane receptor (Latha et al 2008). The domain comprises six tryptophane residues in TsRfBP as in GdRfBP (Hamajima and Ono 1995). This feature suggests that both RfBPs bind to the plasma membrane possibly with an equivalent efficiency.…”

“…On the other hand, the threonine among amino acid residues in the phosphorylated domain is thought to be important for RfBP to bind RIB (Blankenhorn 1978). GdRfBP and TsRfBP both contain eight phosphorylation clusters that comprise nine serine residues and five glutamic acid residues, but TsRfBP has two additional threonine-phosphorylation sites (Hamajima and Ono 1995). This difference suggests that the affinity to RIB may be greater for TsRfBP than GdRfBP.…”

Helix Stabilization in the C-Terminal Peptide of Chicken Riboflavin Carrier Protein Enhances Immunogenicity and Prolongs Contraceptive Potential as an Epitope-Based Vaccine in Female Rats

“…RCP is evolutionarily conserved in a wide variety of species ranging from lower vertebrates to mammals including humans (2)(3)(4)(5). Earlier studies have unequivocally established the importance of this carrier protein to support embryonic growth since immunoneutralization of endogenous maternal RCP with antibodies to the heteroantigen viz.…”