Sequence homology and absence of mRNA defines a possible pseudogene member of the Trypanosoma cruzi gp85/sialidase multigene family

Takle, Garry B.; O'Connor, Jack; Young, Ahn Na; Cross, George A.M.

doi:10.1016/0166-6851(92)90159-h

Cited by 22 publications

(14 citation statements)

References 22 publications

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“…4B). This suggests that the copies of SAP may be concentrated in a few loci, rather than distributed throughout the genome, as is the case in several (4,8,10,13,16,20,35). Taken together, the data from Southern blot hybridization, chromosome mapping, and computational analysis of DNA sequences from T. cruzi DB indicate that SAP genes belong to a relatively small family.…”

Section: Resultsmentioning

confidence: 72%

“…Like other surface protein gene families in T. cruzi, the SAP family contains pseudogenes, which could contribute to the SAP repertoire through recombination, a hypothesis that could explain the existence of numerous pseudogenes of the TS-like family and adenylate cyclase family in the genome of T. cruzi (35,37). Trypanosoma brucei apparently uses defective genes in building bona fide variant surface glycoprotein (30).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization of Serine-, Alanine-, and Proline-Rich Proteins of Trypanosoma cruzi and Their Possible Role in Host Cell Infection

Baida¹,

Santos²,

Carmo³

et al. 2006

Infect Immun

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Section: Resultsmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Serine-, Alanine-, and Proline-Rich Proteins of Trypanosoma cruzi and Their Possible Role in Host Cell Infection

Baida¹,

Santos²,

Carmo³

et al. 2006

Infect Immun

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“…Analysis of the nucleotide sequence from the DNA fragments corresponding to bands 1, 2, and 3 showed that indeed the pBAC52 clone contains only one complete copy of the L1Tc element. Downstream from this element a DNA sequence corresponding to the 5©-region of the L1Tc element (nucleotides 111±324) was found, followed by a sequence with 88% homology with the gene encoding for a protein belonging to the sialidase gene family [25]. This truncated L1Tc copy (accession number AF215898), corresponding to the ribosome mobile element (RIME) homologous region, is flanked by the short direct duplication GTATCTTTG sequence (Fig.…”

Section: Isolation and Characterization Of T Cruzi Genomic Clones Comentioning

confidence: 99%

Genomic clustering of theTrypanosoma cruzi nonlong terminal L1Tc retrotransposon with defined interspersed repeated DNA elements

et al. 2000

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We have analyzed the genomic distribution and organization of the long interspersed nucleotide element (LINE) L1Tc, a nonlong terminal repeat (LTR) retrotransposon of Trypanosoma cruzi. The results indicate that the L1Tc element is dispersed along the parasite genome and that in some regions it is organized in tandem repeats. The data allowed us to define the existence of short direct-repeated sequences flanking the genomic L1Tc elements. Relevant is the finding that the LINE L1Tc is located in genomic regions rich in short interspersed nucleotide elements (SINE)-like sequences. In particular, the L1Tc element is found associated to E13-related sequences, redefined in this work and renamed RS13Tc, and to a newly described RS1Tc sequence. The RS1Tc sequence is present, per haploid genome, in about 3,200 copies. Northern blot analysis showed that the RS1Tc is being transcribed into RNAs of different sizes. The analysis of the chromosomal distribution of these elements in various strains of T. cruzi suggested that this type of clustering might be a common feature of the genome of these parasites.

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“…Several T. cruzi molecules associated to flagellum have been previously described, among them a group of surface proteins of the 160-kDa family, belonging to the TS/gp85 superfamily and sharing 48% identity with gp85 proteins (25,30). These proteins were detected on the cell membrane in the flagellar pocket of bloodstream trypomastigotes (28,30).…”

Section: Discussionmentioning

confidence: 89%

Expression and Cellular Localization of Molecules of the gp82 Family in Trypanosoma cruzi Metacyclic Trypomastigotes

et al. 2007

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A member of the Trypanosoma cruzi gp82 family, expressed on metacyclic trypomastigote surface and identified by monoclonal antibody (MAb) 3F6, plays a key role in host cell invasion. Apart from the gp82 defined by MAb 3F6, no information is available on members of this protein family. From cDNA clones encoding gp82 proteins sharing 59.1% sequence identity, we produced the recombinant proteins J18 and C03, the former containing and the latter lacking the epitope for MAb 3F6. Polyclonal antibodies to J18 and C03 proteins were generated and used, along with MAb 3F6, to analyze the expression and cellular localization of gp82 family members in metacyclic forms of CL and G strains, which belong to highly divergent genetic groups. By two-dimensional gel electrophoresis and immunoblotting, molecules of 82 to 86 kDa, focusing at pH 4.6 to 5.4, and molecules of 72 to 88 kDa, focusing at pH 4.9 to 5.7, were visualized in CL and G strains, respectively. Flow cytometry and microscopic analysis revealed in both strains similar expression of MAb 3F6-reactive gp82 in live and permeabilized parasites, indicating its surface localization. The reaction of live parasites of both strains with anti-J18 antibodies was weaker than with MAb 3F6 and was increased by permeabilization. Anti-C03 antibodies bound predominantly to flagellar components in permeabilized G strain parasites, but in the CL strain the flagellum was not the preferential target for these antibodies. Host cell invasion of metacyclic forms was inhibited by J18 protein, as well as by MAb 3F6 and anti-J18 antibodies, but not by C03 protein or anti-C03 antibodies.

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Sequence homology and absence of mRNA defines a possible pseudogene member of the Trypanosoma cruzi gp85/sialidase multigene family

Cited by 22 publications

References 22 publications

Molecular Characterization of Serine-, Alanine-, and Proline-Rich Proteins of Trypanosoma cruzi and Their Possible Role in Host Cell Infection

Molecular Characterization of Serine-, Alanine-, and Proline-Rich Proteins of Trypanosoma cruzi and Their Possible Role in Host Cell Infection

Genomic clustering of theTrypanosoma cruzi nonlong terminal L1Tc retrotransposon with defined interspersed repeated DNA elements

Expression and Cellular Localization of Molecules of the gp82 Family in Trypanosoma cruzi Metacyclic Trypomastigotes

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