Sequence diversity on four ORFs of citrus tristeza virus correlates with pathogenicity

Herrera-Isidrón, Lisset; Ochoa-Sánchez, Juan Carlos; Rivera-Bustamante, R. F.; Martı́nez-Soriano, Juan Pablo

doi:10.1186/1743-422x-6-116

Cited by 17 publications

(17 citation statements)

References 16 publications

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“…GLRaV-2 sequences were classified into six genetic groups including isolates from: (I) China, USA, Brazil, Italy, Poland, and South Africa, (II) Italy and South Africa, (III) USA, Italy, and Brazil, (IV) Italy and France, (V) USA, and (VI) Italy (Figure 1C). Finally, the citrus-infecting CTV, the best studied virus in the family, with CP sequences of almost 600 worldwide isolates (Albiach-Martí et al, 2000; Rubio et al, 2001b; Alavi et al, 2005; Roy et al, 2005; Sorrentino et al, 2005; Papayiannis et al, 2007; Iglesias et al, 2008; Jiang et al, 2008; Černi et al, 2009; Herrera-Isidrón et al, 2009; Oliveros-Garay et al, 2009; Fisher et al, 2010; Harper et al, 2010; Matos et al, 2013), showed a moderate genetic variability. All isolates fell into a single genetic group, albeit this could be divided into five to seven subgroups (Figure 1C), some of them very homogeneous (isolates with almost identical sequences).…”

Section: Resultsmentioning

confidence: 99%

Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae

2013

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RNA viruses have a great potential for genetic variation, rapid evolution and adaptation. Characterization of the genetic variation of viral populations provides relevant information on the processes involved in virus evolution and epidemiology and it is crucial for designing reliable diagnostic tools and developing efficient and durable disease control strategies. Here we performed an updated analysis of sequences available in Genbank and reviewed present knowledge on the genetic variability and evolutionary processes of viruses of the family Closteroviridae. Several factors have shaped the genetic structure and diversity of closteroviruses. (I) A strong negative selection seems to be responsible for the high genetic stability in space and time for some viruses. (2) Long distance migration, probably by human transport of infected propagative plant material, have caused that genetically similar virus isolates are found in distant geographical regions. (3) Recombination between divergent sequence variants have generated new genotypes and plays an important role for the evolution of some viruses of the family Closteroviridae. (4) Interaction between virus strains or between different viruses in mixed infections may alter accumulation of certain strains. (5) Host change or virus transmission by insect vectors induced changes in the viral population structure due to positive selection of sequence variants with higher fitness for host-virus or vector-virus interaction (adaptation) or by genetic drift due to random selection of sequence variants during the population bottleneck associated to the transmission process.

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Section: Resultsmentioning

confidence: 99%

Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae

2013

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“…The risks of spreading these severe isolates to new areas by propagation or nursery trees are real and should be considered (Herrera-Isidrón et al, 2009). Therefore, losses can only be effectively reduced and prevented by an efficient system of preimmunization against these severe isolates (Sambade et al, 2002;Moreno et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program

Zanutto

Corazza

Nunes

et al. 2013

Sci. agric. (Piracicaba, Braz.)

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The use of tolerant rootstocks and preimmunization has satisfactorily controlled losses associated with the Citrus tristeza virus (CTV). Several researchers have shown that CTV mild isolates that are selected in the same region where they are used are superior to isolates obtained from other areas. Thus, budwoods of 20 outstanding citrus trees were collected in north and northwestern Paraná state (Brazil) citrus-producing areas and established to be used in a preimmunization program. These budwoods were tested to evaluate the potential protection of the inherently present viral complex. Based on biological indexing and molecular characterization of the capsid protein gene by RFLP (restriction fragment length polymorphism, which indicated that the plants were infected with mild isolates of CTV), some of the selected plants could be used in a preimmunization program. These potentially mild and protective isolates were challenged with severe 'Rolândia' isolate inoculations by grafting and by the brown citrus aphid (Toxoptera citricida Kirkaldy) vector, which was faster in transmitting the virus. Some isolates had a better protective value than others, particularly when challenged with the severe CTV isolate. The SSCP (single strand conformational polymorphism) molecular analysis was an excellent complementary tool for monitoring the performance of the experiments and the stability of the viral complex present in the plants.Isolate number 1, collected in the municipality of Cruzeiro do Sul (CS-1), was the most promising for protecting commercial Pêra sweet orange (C. sinensis L.) orchards in northern and northwestern Paraná. The Rolândia severe CTV isolate was stable and had a high genetic divergence among the severe isolates used as a control (Capão Bonito and Barão B) and all of the isolates tested.

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“…For amplification of the CP genomic region (672 bp), reverse transcription polymerase chain reaction (RT-PCR) with set of primers IRA1 5'ATGGACGAC-GAAACAAAGAAATTG3' and IRA2 5'GCTCAACGTGTGTTAA3' [16] was used. To generate the cDNA, approximately 200 ng of total RNA were denatured at 95˚C for 5 min.…”

Section: Amplification Of the Cp Genomic Regionmentioning

confidence: 99%

“…The maximum parsimony and Bayesian inference method were used to generate the phylogenetic tree for the CP region including the following full length CTV sequences: T36 [11] and T30 from Florida [29], VT from Israel [30], T385 from Spain [31], SY568 from California [32], NUagA from Japan [33], CTV from Mexico [34], NZ-M16 and NZ-B18 from New Zealand [35]; and CP sequences from Mexico: TAM11, NL8, VER2-2, QR2753-1, BC15-1, MichL9A11/9-1, Colima 1997 and Yucatán 2000 [16].…”

Section: Phylogeny Of Ctv Isolatesmentioning

confidence: 99%

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Severe <i>Citrus tristeza virus</i> Isolates from Eastern Mexico Are Related to the T36 Genotype Group

Rivas-Valencia¹,

Domínguez-Monge²,

Santillán-Mendoza³

et al. 2020

AJPS

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Citrus tristeza virus (CTV) outbreaks have been reported in the main citrus growing region of Mexico in the past four years. Recently, in eastern Mexico (the major citrus-growing region in the country), severe CTV isolates have been detected. However, the molecular identity of observed isolates remains unestablished. This research was undertaken to elucidate the molecular characterization of CTV populations spreading in this region and to compare it with phylogeny of existing isolates. Genotyping of 32 collected isolates was performed using reverse-transcription polymerase chain reaction (RT-PCR) with sequence analysis of the coat protein (CP) gene, putatively associated with pathogenicity. This protein is a 25 kDa major capsid protein, which forms a long virion body coating 95% of the particle length. A comparative sequence analysis was performed using CTV sequences from different geographical origins already published and deposited in the GenBank databases. Phylogenetic analysis showed that the degree of sequence divergence among isolates correlated with their pathogenicity. Based on the sequencing results, the collected isolates were categorized as mild or severe phylogenetic clusters, each being genetically distinct. The severe group was associated with either alike or with a T36-like genotype. The latter group matched with the quick decline and stem pitting drastic symptoms observed in the field. This study

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Sequence diversity on four ORFs of citrus tristeza virus correlates with pathogenicity

Cited by 17 publications

References 16 publications

Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae

Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae

Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program

Severe <i>Citrus tristeza virus</i> Isolates from Eastern Mexico Are Related to the T36 Genotype Group

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Sequence diversity on four ORFs of citrus tristeza virus correlates with pathogenicity

Cited by 17 publications

References 16 publications

Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae

Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae

Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program

Severe &lt;i&gt;Citrus tristeza virus&lt;/i&gt; Isolates from Eastern Mexico Are Related to the T36 Genotype Group

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Severe <i>Citrus tristeza virus</i> Isolates from Eastern Mexico Are Related to the T36 Genotype Group