Sequence Diversity of the Plasmid Replication Gene repC in the Rhizobiaceae

Palmer, K. M.; Turner, Sarah L.; Young, J. Peter W.

doi:10.1006/plas.2000.1488

Cited by 33 publications

(25 citation statements)

References 32 publications

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“…The conjugal transfer (tra) system of pRi1724 shows closer similarity to the tra system of the sequenced Sinorhizobium Sym plasmid pNGR234 (6) and to some plasmids in R. leguminosarum (29) than to the tra systems in several Ti plasmids (16). The plasmid replication genes of Agrobacterium plasmids are also related to those of rhizobia (21). A Rhizobium symbiotic plasmid (pSymG100) has been shown to belong to the same incompatibility class as the Agrobacterium Ri plasmid (pRi1855) (18), indicating that a self-transmissible RMA pRi could disseminate to other ␣-Proteobacteria.…”

Section: Discussionmentioning

confidence: 98%

Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other α- Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat

Weller¹,

Stead²,

Young

2004

Appl Environ Microbiol

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Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring ␣-Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five as being in the genus Rhizobium, and one as being in the genus Sinorhizobium. An in vitro pathogenicity test involving inoculation of cucumber cotyledons was developed. All pRi-harboring ␣-Proteobacteria induced typical root mat symptoms from the cotyledons. Average transformation rates for rhizogenic Ochrobactrum (46%) and Rhizobium (44%) strains were lower than those observed for rhizogenic A. radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculated with a rhizogenic Sinorhizobium strain (75%

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Section: Discussionmentioning

confidence: 98%

Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other α- Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat

Weller¹,

Stead²,

Young

2004

Appl Environ Microbiol

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“…Analyses of RepABC proteins were also proven to be valuable for assessment of the phylogenetic relatedness among the rhizobial plasmids [43,44]. Despite the wide distribution and importance of plasmids for rhizobia, their evolutionary history has been incompletely explored, mainly because of the lack of extensive similarity between plasmids, with the exception of replication and transfer function [45].…”

Section: Rhizobial Plasmid Maintenance Systemsmentioning

confidence: 99%

Rhizobial plasmids — replication, structure and biological role

Mazur

Koper

2012

Open Life Sciences

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AbstractSoil bacteria, collectively named rhizobia, can establish mutualistic relationships with legume plants. Rhizobia often have multipartite genome architecture with a chromosome and several extrachromosomal replicons making these bacteria a perfect candidate for plasmid biology studies. Rhizobial plasmids are maintained in the cells using a tightly controlled and uniquely organized replication system. Completion of several rhizobial genome-sequencing projects has changed the view that their genomes are simply composed of the chromosome and cryptic plasmids. The genetic content of plasmids and the presence of some important (or even essential) genes contribute to the capability of environmental adaptation and competitiveness with other bacteria. On the other hand, their mosaic structure results in the plasticity of the genome and demonstrates a complex evolutionary history of plasmids. In this review, a genomic perspective was employed for discussion of several aspects regarding rhizobial plasmids comprising structure, replication, genetic content, and biological role. A special emphasis was placed on current post-genomic knowledge concerning plasmids, which has enriched the view of the entire bacterial genome organization by the discovery of plasmids with a potential chromosome-like role.

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“…The sole representative of this class of plasmids that has been identified outside the Rhizobiaceae group is pTAV1 (mini-replicon pTAV320) of the soil bacterium Paracoccus versutus (5). pTAV320 appears to be the most divergent member of this family (5,21,32). pTAV1 is a composite plasmid, and in addition to pTAV320 it codes for a second replicator region (not included in the repABC class) that has been cloned in the form of mini-replicon pTAV202 (3, 7).…”

mentioning

confidence: 99%

“…The repABC family of replicons is widely distributed in bacteria belonging to the genera Rhizobium and Agrobacterium (32,35). Several repABC replicons have been sequenced so far, including the root-inducing plasmids pRiA4b (29) and pRi1724 (28) of Agrobacterium rhizogenes; the tumor-inducing plasmids pTiB6S3 (39), pTi-SAKURA (38), pTiC58 (21), and pTiBo542 (containing in a tandem repeat two phylogenetically distinct repABC-type replicons) (31) of Agrobacterium tumefaciens; symbiotic plasmids pNGR234-a of Rhizobium spp.…”

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confidence: 99%

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Identification of the Partitioning Site within the repABC- Type Replicon of the Composite Paracoccus versutus Plasmid pTAV1

2001

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The replicator region of composite plasmid pTAV1 of Paracoccus versutus (included in mini-replicon pTAV320) belongs to the family of repABC replicons commonly found in plasmids harbored by Agrobacterium and Rhizobium spp. The repABC replicons encode three genes clustered in an operon, which are involved in partitioning (repA and repB) and replication (repC). In order to localize the partitioning site of pTAV320, the two identified incompatibility determinants of this mini-replicon (inc1, located in the intergenic sequence between repB and repC; and inc2, situated downstream of the repC gene) were PCR amplified and used together with purified RepB fusion protein (homologous to the type B partitioning proteins binding to the partitioning sites) in an electrophoretic mobility shift assay. The protein bound only inc2, forming two complexes in a protein concentration-dependent manner. The inc2 region contains two long (14-bp) repeated sequences (R1 and R2). Disruption of these sequences completely eliminates RepB binding ability. R1 and R2 have sequence similarities with analogous repeats of another repABC replicon of plasmid pPAN1 of Paracoccus pantotrophus DSM 82.5 and with centromeric sequences of the Bacillus subtilis chromosome. Excess RepB protein resulted in destabilization of the inc2-containing plasmid in Escherichia coli. On the other hand, the inc2 region could stabilize another unstable replicon in P. versutus when RepA and RepB were delivered in trans, proving that this region has centromere-like activity. Thus, it was demonstrated that repA, repB, and inc2 constitute a functional system for active partitioning of pTAV320.Low-copy-number plasmids code for partitioning systems that ensure the correct distribution of plasmids within a bacterial population by precisely separating newly replicated copies into daughter cells at cell division. In general, these systems consist of three elements: (i) a trans-acting type A protein (ATPase) which autoregulates the operon and (ii) a trans-acting type B protein which binds to (iii) a cis-required partitioning site, which is thought to be analogous to the centromeres of eukaryotic chromosomes (27, 41). All three elements are indispensable for the proper functioning of these systems.Although the partitioning systems are very often encoded close to plasmid replicator regions, they have been shown to function as independent cassettes that are able to stabilize (in cis) other, even unrelated replicons (30, 41). So far, there has been no evidence of structural association and coregulation of the two most important maintenance mechanisms for plasmids, replication and partitioning. The so-called repABC-type replicons seem to be an exception as the genes involved in the two processes are organized in an operon (5, 34).The repABC family of replicons is widely distributed in bacteria belonging to the genera Rhizobium and Agrobacterium (32, 35). Several repABC replicons have been sequenced so far, including the root-inducing plasmids pRiA4b (29) and pRi1724 (28) of Agrobacteri...

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Sequence Diversity of the Plasmid Replication Gene repC in the Rhizobiaceae

Cited by 33 publications

References 32 publications

Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other α- Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat

Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other α- Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat

Rhizobial plasmids — replication, structure and biological role

Identification of the Partitioning Site within the repABC- Type Replicon of the Composite Paracoccus versutus Plasmid pTAV1

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